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Metatranscriptome Sequencing
Metatranscriptome sequencing fa'ailoaina le fa'aaliga o kenera o microbes (eukaryotes uma ma prokaryotes) i totonu o si'osi'omaga fa'anatura (e pei o le palapala, vai, sami, feces, ma gut.) Aemaise lava, o nei 'au'aunaga e mafai ai ona e maua atoa fa'amatalaga fa'amatalaga fa'amatalaga o nu'u lavelave microbial, iloiloga taxonomic o ituaiga, su'esu'ega fa'atamaoaigaina o ga'o eseese fa'aalia, ma isi mea.
Tulaga: Illumina NovaSeq Platform
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Fungal Genome
Biomarker Technologies e tuʻuina atu suʻesuʻega o le genome, genome lelei ma faʻamaeʻaina genome o gaʻo e faʻatatau ile sini suʻesuʻe.Genome sequencing, faʻapotopotoga ma faʻamatalaga aoga e mafai ona ausia e ala i le tuʻufaʻatasia o le Generation sequencing + Faʻasologa o augatupulaga lona tolu e ausia ai le maualuga o le genome assembly.E mafai fo'i ona fa'aogaina le tekonolosi Hi-C e fa'afaigofie ai le fa'apotopotoina o le genome ile tulaga ole chromosome.
Fa'avae:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Meafaigaluega
BMKCloud o se taʻutaʻua bioinformatic faʻavae e tuʻuina atu ai le tasi-stop solution mo polokalame genomic, lea e faʻatuatuaina lautele e tagata suʻesuʻe i malae eseese e aofia ai fomaʻi, faʻatoʻaga, siosiomaga, ma isi. , punaoa faakomepiuta, faʻamaumauga a le lautele, bioinformtic online courses, etc. BMKCloud o loʻo i ai le tele o mea e masani ona faʻaaogaina bioinformatic e aofia ai faʻamatalaga gene, meafaigaluega faʻasolosolo faʻasolosolo, ncRNA, faʻatonuga lelei o faʻamaumauga, faʻapotopotoga, faʻaogaina, faʻapipiʻiina o faʻamatalaga, suiga, fuainumera, faʻasologa o ata, faʻasologa auiliiliga, ma isi.
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Bacteria Complete Genome
Biomarker Technologies e tu'uina atu auaunaga fa'asologa i le fausiaina o genome atoatoa o siama ma leai se avanoa.O galuega autu o siama faʻamaeʻaina fau genome e aofia ai le faʻasologa o augatupulaga lona tolu, faʻapipiʻi, faʻamatalaga galuega ma suʻesuʻega bioinformatic alualu i luma faʻataunuʻuina sini maoti suʻesuʻega.O se fa'amatalaga sili atu ona manino o le genome siama e fa'amalosia ai le fa'aaliaina o faiga fa'avae o lo'o fa'avaeina a latou gaioiga fa'ale-natura, lea e mafai fo'i ona maua ai fa'amatalaga taua mo su'esu'ega genomic i ituaiga eukaryotic maualuga.
Fa'avae:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
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RNA laiti
O tama'i RNA o se ituaiga o RNA e le fa'ailogaina ma le umi ole 18-30 nt, e aofia ai le miRNA, siRNA ma le piRNA.O nei tama'i RNA ua fa'ailoa tele e a'afia i faiga fa'ale-agai eseese e pei o le fa'aleagaina o le mRNA, fa'alavelave fa'aliliuga, fa'avaeina o le heterochromatin, ma isi. O su'esu'ega fa'asologa o SmallRNA ua fa'aaogaina lautele i su'esu'ega i le atina'eina o manu/laau, fa'ama'i, siama, ma isi mea laiti RNA. fa'asologa o su'esu'ega fa'asologa o lo'o i ai fa'amaumauga masani ma le fa'aogaina o fa'amaumauga.I luga o faʻavae o faʻamaumauga RNA-seq, suʻesuʻega masani e mafai ona ausia le faʻamaoniga ma le vavalo o le miRNA, faʻasologa o faʻasologa o le miRNA, faʻamatalaga ma faʻamatalaga faʻamatalaga.O su'esu'ega fa'apitoa e mafai ai ona su'esu'eina ma fa'aputuina le miRNA, fa'atupuina o ata o Venn, miRNA ma le fausiaina o feso'ota'iga gafa.
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NGS-WGS (Illumina/BGI)
NGS-WGS ose fa'asologa o su'esu'ega fa'asologa atoa o le genome, lea e atia'e i luga o fa'avae o le poto masani i Biomarker Technologies.O lenei fa'aoga faigofie e mafai ai ona vave tu'uina atu se su'esu'ega tu'ufa'atasiga e ala i le fa'atulagaina o nai fa'amaufa'ailoga autu, lea e fetaui mo fa'amaumauga fa'asologa o DNA e maua mai i le Illumina platform ma le BGI sequencing platform.O lenei fa'avae o lo'o fa'apipi'iina i luga o le 'au'aunaga fa'akomepiuta maualuga, lea e fa'amalosia ai su'esu'ega fa'amaumauga i se taimi fa'atapula'a.O lo'o maua le la'uina o fa'amatalaga fa'apitoa i luga o le fa'avae o su'esu'ega masani, e aofia ai su'esu'ega fa'asologa o gene, mamanu muamua o le PCR, ma isi.
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mRNA(Fa'asinoga)
Transcriptome o le feso'ota'iga i le va o fa'amatalaga fa'a-genomic ma le puipuiga o galuega fa'aola.O tulafono faatonutonu tulaga fa'aliliu e sili ona taua ma sili ona su'esu'eina fa'atonuga o meaola.Transcriptome sequencing e mafai ona faʻasolosolo le transcriptome i soʻo se taimi i le taimi poʻo lalo o soʻo se tulaga, faʻatasi ai ma se iugafono saʻo i se nucleotide e tasi. fa'ata'ita'i tusitusiga fa'apitoa.
I le taimi nei, transcriptome sequencing tekinolosi ua faʻaaogaina lautele i agronomy, vailaʻau ma isi suʻesuʻega, e aofia ai tulafono faʻatupuina o manu ma laau, fetuunaiga o le siosiomaga, fesoʻotaʻiga puipuia, gene localization, ituaiga genetic evolution ma tumo ma faʻamaʻi faʻamaʻi.
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Metagenomics (NGS)
O lenei fa'avae au'ili'ili ua mamanuina mo fana fana metagenomic su'esu'ega fa'amaumauga i luga o le fa'avae o tausaga o le poto masani.O lo'o i ai fa'atasi galuega fa'aogaina o lo'o iai su'esu'ega metagenomics e masani ona mana'omia e aofia ai su'esu'ega fa'amaumauga, su'esu'ega tulaga-tulaga, su'esu'ega tulaga-tulaga o le gene, metagenome binning, ma isi. , fa'atulagaina fa'amaufa'ailoga, fa'atupuina fa'atusa fa'apitoa, ma isi.
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LncRNA
O RNA e le fa'ailoga umi (lncRNA) o se ituaiga o tusitusiga e umi atu nai lo le 200 nt, e le mafai ona fa'ailoga polotini.O fa'amaumauga fa'aputuga e fa'ailoa mai ai o le tele o lncRNAs e foliga mai e fa'atino.Fa'atekonolosi fa'asologa maualuga ma mea faigaluega su'esu'e bioinformatic e fa'amalosia ai i tatou e fa'aalia fa'asologa o le lncRNA ma fa'atulagaina fa'amatalaga sili atu ona lelei ma ta'ita'ia ai i tatou e su'e lncRNA ma galuega fa'atonutonu taua.E mitamita le BMKCloud e tu'uina atu ia matou tagata fa'atau le lncRNA sequencing analysis platform e ausia ai le vave, fa'atuatuaina ma fetu'una'i su'esu'ega lncRNA.
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GWAS
Genome-wide association suʻesuʻega (GWAS) e faʻamoemoe e faʻamaonia fesuiaiga o kenera (genotype) e fesoʻotaʻi ma uiga faʻapitoa (phenotype).O su'esu'ega a le GWA e su'esu'e ai fa'ailoga fa'a kenera e fa'asaga i le genome atoa o le aofa'i tele o tagata ta'ito'atasi ma vavalo fa'atasiga fa'atupu-fa'ailoga e ala i su'esu'ega fa'afuainumera i le aofa'i o tagata.Ole su'esu'ega o le genome atoa e mafai ona maua ai fa'asologa o kenera uma.Fa'atasi ma fa'amaumauga fa'apitoa, e mafai ona fa'agasolo le GWAS e fa'ailoa ai SNP e feso'ota'i phenotype, QTLs ma genes sui, lea e fa'aola malosi ai le fa'atupuina o manu/laau fa'aonaponei.O le SLAF o se ta'iala e fa'atupuina e le tagata lava ia fa'afaigofie fa'asologa o le genome, lea e maua ai fa'ailoga fa'asalalauina lautele, SNP.O nei SNP, e pei o faʻailoga mole mole, e mafai ona faʻatautaia mo suʻesuʻega faʻatasi ma uiga faʻatatau.Ose ta'iala taugofie i le fa'ailoaina o uiga lavelave e feso'ota'i ma fesuiaiga o kenera.
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Nanopore Fa'amaumauga atoa
Lavelave ma fesuia'i isi isoforms i meaola o ni mea taua tele mo le fa'atonutonuina o fa'amatalaga o kenera ma le eseesega o porotini.O le faʻamaonia saʻo o faʻasologa o tusitusiga o le faʻavae lea mo suʻesuʻega loloto o faʻataʻitaʻiga o tulafono faatonutonu o faʻamatalaga.Nanopore sequencing platform ua manuia le aumaia o suʻesuʻega transcriptomic i le isoform-level.O lenei fa'avae au'ili'ili ua mamanuina e fa'avasega ai fa'amaumauga a le RNA-Seq na fa'atupuina ile Nanopore platform ile fa'avae ole genome fa'asinomaga, lea e ausia ai au'ili'iliga fa'atatau ma fa'atusatusaga ile fa'asologa o gene ma le fa'asologa o tusitusiga.
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li'o-RNA
Circular RNA(circRNA) o se ituaiga o RNA e le-coding, lea e le'i leva ona maua e faia se sao taua i fesoʻotaʻiga faʻatonutonu o loʻo aʻafia i le atinaʻeina, teteʻeina o le siosiomaga, ma isi. E ese mai mole RNA laina, eg mRNA, lncRNA, 3' ma le 5' pito o le circRNA o loʻo tuʻufaʻatasia e fausia ai se fausaga lapotopoto, e faʻasaoina ai i latou mai le faʻaogaina o le exonuclease ma e sili atu ona mautu nai lo le tele o laina RNA.Ua maua le CircRNA e iai galuega eseese i le faʻatonutonuina o faʻamatalaga o kenera.O le CircRNA e mafai ona faʻatinoina e pei o le ceRNA, lea e fusifusia ai le miRNA faʻatauva, e taʻua o le miRNA sponge.CircRNA fa'asologa o su'esu'ega fa'avae e fa'amalosia ai le fa'atulagaina o le circRNA ma fa'amatalaga fa'amatalaga, va'aiga fa'amoemoe ma su'esu'ega fa'atasi ma isi ituaiga mole RNA.
