Fa'asologa o Genome Atoa Fa'ama'i ma Fungal
Poloketi toe fa'asolosolo fa'asologa o siama ma fungal e taua tele mo le fa'alauteleina o genomics microbial e ala i le mafai ona fa'amae'aina ma fa'atusatusaga o genom microbial. Ole mea lea e fa'afaigofieina ai le fa'ainisinia fa'apa'u, le fa'asilisiliina ole faiga ole alamanuia, ma le su'esu'eina ole auala lona lua ole metabolism. E le gata i lea, e taua tele le toe faʻasologa o siama ma siama mo le malamalama i fetuunaiga o le siʻosiʻomaga, faʻamalieina o faʻalavelave, ma faʻaalia le malosi o le evolusione, faʻatasi ai ma le lautele o aʻafiaga i vailaʻau, faʻatoʻaga, ma le siosiomaga faasaienisi.
Tulaga Auaunaga
●Su'esu'ega Bioinformatic E aofia ai Valaauga Eseese:Tu'uina atu fa'amatalaga fa'atino ile genomes toe fa'asologa.
● Tomai Tele: Faatasi ai ma le faitau afe o microbial re-sequencing poloketi faʻatautaia i tausaga taʻitasi, matou te aumaia le sili atu ma le sefulu tausaga o le poto masani, o se au suʻesuʻe sili ona tomai, faʻamatalaga atoatoa, ma lagolago sili ona lelei pe a maeʻa faʻatau.
●Lagolago ina ua uma ona faatau atu:O la matou tautinoga e faʻalautele atu i tua atu o le faʻamaeʻaina o galuega faʻatasi ma le 3-masina pe a maeʻa le faʻatau atu vaitaimi auaunaga. I le taimi lea, matou te ofoina atu le tulitatao o poloketi, fesoasoani faʻafitauli, ma Q&A sauniga e faʻafeiloaʻi ai soʻo se fesili e fesoʻotaʻi ma iʻuga.
Fa'amatalaga Auaunaga
| Fa'asologa fa'asologa | Fuafuaga Fa'asologa | Fa'amatalaga fautuaina | Puleaina lelei |
| Illumina NovaSeq | PE150 | Le loloto o le 100x | Q30≥85% |
Fa'ata'ita'iga Manaoga
| Fa'atotonuga (ng/µL) | Aofa'iga (ng) | Volume (µL) |
| ≥1 | ≥60 | ≥20 |
siama: ≥1x107 sela
Ga'ai Tu'atasi: ≥5x106-1x107 sela
Fa'aliga Matagofie: ≥4g
Auala o Galuega Au'aunaga
Fa'ata'ita'iga tu'uina atu
Fauga faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
E aofia ai le Su'esu'ega o lo'o mulimuli mai:
- Fa'asologa o Fa'amaumauga Tulaga Lelei
- Fa'atatau ile Fa'asinoga Genome
- Valaauga eseese: SNP ma InDel
- Eseese Annotation
Valaau eseese: SNP ituaiga
Valaau ese'ese: Fa'asoa umi InDel
Su'esu'e le aga'i i luma ua fa'afaigofieina e le BMKGene's microbial genome re-sequencing services e ala i le aoina o fa'asalalauga.
Jia, Y. et al. (2023) 'Tu'ufa'atasia o le Transcriptome ma le Genome Atoa Toe Fa'asologa e Su'e Fa'ama'i Tete'e Genes mo Wheat Dwarf Bunt',Tusitala fa'ava-o-malo o su'esu'ega mole, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Ampicillin-controlled glucose metabolism e faʻaogaina le suiga mai le faapalepale i le tetee i siama',Fa'asaienisi Agai i luma, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalkaliphila sp. Nov., o se siama haloalkaliphilic e vavae ese mai se vaituloto i totonu o Mongolia Autonomous Region, Saina', Int. J. Syst. Evol.Microbiol, 72, itulau. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. ma Wang, G.-H. (2024) 'Genome sequence o Staphylococcus nepalensis ZZ-2023a, vavae ese mai Nasonia vitripennis',Microbiology Resource Announcements. doi: 10.1128/MRA.00802-23.
