16s / 18s / lona ammlick sequeck-ngs
Tautua o foliga
● Sequening platform: imomurina novaseq.
● Amplication o le puʻupuʻu o le 16s, 18s ma lana, i totonu o isi faʻataʻitaʻiga.
● ● O filifiliga ese o apepogi.
● O le poto masani na maua ai le poto masani ma le tele o le amplication sini.
Tulaga lelei
●Leai se fua faatatau:O le saoasaoa o le faʻailoaina o MicroBal vaega i le siosiomaga faʻataʻitaʻiga.
●Maualuga iugafono: I le maualalo-tele vaega o le siosiomaga faʻataʻitaʻiga.
●Lautele talafeagai: Eseese le tele o le va fealoai nuu.
●Malamalamaaga o le bionigatanatic auiliiliga iloiloga: O le lata mai QiIme2 afifi (aofaʻiga malamalama i le microbial ecology) ma le tele o aʻoaʻoga i tulaga o faʻamaumauga, otu.
●Lautele tomai faapitoa: Ma le 150 afe o le faʻaopoopoina o poloketi faʻaopopoina poloketi na faia i tausaga taʻitasi, BMKGEne e aumaia se sefulu tausaga o le poto masani
Auaunaga Mataupu Faavae
| Faletusi | SUʻESUʻEGA FAʻATASI | Faamatalaga Fautuaina |
| Am4con | Issumina Pe250 | 50/100 / 300k pine (Faitau paga) |
Tautua Tautua
| Manatu (ng / μl) | Aofai aofaiga (ng) | Voluma (μl) |
| ≥1 | ≥200 | ≥20 |
● O le eleele / speedge: 1-2G
● Actisil Anos-manu: 0,5-2G
● O mea le aoga
● totoga (faʻamalosia le fao): 0.10.5g
● Faʻasaina o le faʻafefeteina o le faʻaʻaisa): 0.10.5g
● Fana (manu tetele): 0.5-2G
● Faces (isumu): 3-5clakins
● Pulumory Alveolalar lavage vai: faamama pepa
● vaginal swab: 5-6 swabs
● Paʻu / Cloy Commit Swab / Saliva / Tuʻufaʻatasi Smashed Tisue / Paryngel Swab / Reckal Swab: 2-3 Swabs
● luga Microorleissisms: faamama pepa
● O le vai o le vai / Air / BioFilm: faamama pepa
● Faʻaiuga: 1-2G
● dental plaque: 0.5-1G
Auaunaga galue
Faataʻitaʻiga Faafou
Fausiaina o le Faletusi
Liu
Faamatalaga o faamatalaga
A maeʻa-faʻatau auaunaga
E aofia ai le auiliiliga lenei:
- Raw faʻamatalaga faʻamatalaga lelei
- OTU Clisterting / De-Beao (Asv)
- OTU pronovetion
- Alifa eseese suʻesuʻega: tele atu igoa, aofia ai ma Shannon, Simpson ma le ACE.
- O le ATA DEVESTRESSE SUʻESUʻEGA
- Suesuega Suesuega
- Faʻaopoopoina o Suʻesuʻega: i le va o mea siosiomaga
- 16s le aoga a Gene
Histogram o le sonetoomic tufatufaina
Faapaleaina o le Lafoga o Lafoga
O le auiliiliga alpha eseese: Farrefaction curve
bota eseese auiliiliga: NMDS
Intergroup auiliiliga: Lefse Biomarch maua maua
Saili i alualu i luma faʻafaigofie a le Bmkgene's Amplion o le faʻasologa o tautua ma imaumina e ala i se faʻalumaina aoina o faʻasalalauga.
Dong, c. et al. (2022) 'Fono le maʻau, Coer Microbiota, ma le galuega a le RhizoSpher Ili o Microbiota i le Eucommal, 13. DOI: 43389 /)).
Li, Y. Et al. (2023) 'Synthetic Bacterial Consortia o loʻo faʻaulu mai mo le togafitiga o Gardnerella Vaigatus-faaosofia le Bacteral Viances Viagisis i Mice', 11 (1), PP. 1 (1) DoI: 10.1186 / S40168-023-01497-y
Yang, J., fu, y. ma Li, H. (2022) 'Microbiomes Roonneration Carectives, 17 (1) 1-20. DoI: 10.1186 / S40793-022-00399-0 / fuainumera / 8.
Yin, S. Et al. (2022) 'Fafaga-faʻalagolago tele-faʻalagolago tele o General General e fesoʻotaʻi ma nitrogen suia faʻatonutonuina ni tagata faʻamasino 127678. DOI: 10.1016 / J.biirteech.2022.127678.
