Metagenomic Sequencing -NGS
O le metagenome o se fa'aputuga o le aofa'i o mea tau kenera o se fa'alapotopotoga fefiloi o meaola, e pei o metagenomes o le si'osi'omaga ma tagata. O lo'o iai genomes o meaola ninii e mafai ona fa'ato'aina ma mea e le mafai ona fa'ato'aina. Shotgun metagenomic sequencing ma NGS e mafai ai ona suʻesuʻeina nei laufanua faʻatupu faʻamaʻi faʻapipiʻi i totonu o faʻataʻitaʻiga o le siʻosiʻomaga e ala i le tuʻuina atu o le sili atu nai lo le faʻavasegaina o lafoga, e tuʻuina atu ai foʻi faʻamatalaga manino i ituaiga eseese, faʻateleina dynamics, ma fausaga faʻalavelave faitau aofaʻi. I tua atu o su'esu'ega fa'aletupe, fa'apena metagenomics o lo'o ofoina mai ai fo'i se va'aiga genomics fa'atino, e mafai ai ona su'esu'eina genes ua fa'asalaina ma a latou matafaioi fa'atatau i faiga fa'alesiosiomaga. Mulimuli ane, o le fa'avaeina o feso'ota'iga feso'ota'iga i le va o elemene kenera ma mea tau si'osi'omaga e saofagā i se malamalama'aga atoa o le feso'ota'iga lavelave i le va o nu'u microbial ma o latou si'osi'omaga. I le faaiuga, o le metagenomic sequencing e tu o se meafaigaluega taua mo le tatalaina o le genomic intricacies o microbial community eseese, faʻamalamalamaina le tele o fesoʻotaʻiga i le va o genetics ma le siʻosiʻomaga i totonu o nei faʻalavelave faʻanatura.
Tulaga: Illumina NovaSeq ma DNBSEQ-T7
Tulaga Auaunaga
●Tu'uesea ma Fa'ato'aga-leai se auala mo Microbial Community Profiling: Fa'aagaaga le fa'asologa o mea fa'atupu mai meaola e le mafai ona fa'ato'aina.
●Filifiliga Maualuga: Su'esu'e ituaiga e maualalo le tele i fa'ata'ita'iga o le si'osi'omaga.
●Au'ili'iliga o Bioinformatics:O lo'o taula'i atu e le gata i le eseesega o lafoga ae fa'apea fo'i i le fa'atinoga o galuega a le nu'u.
●Aafiaga tele:Faatasi ai ma se faamaumauga o le tapunia manuia o le tele o metagenomics poloketi i vaega eseese o suʻesuʻega ma faʻagaioiga i luga ole 200,000 faʻataʻitaʻiga, o la matou 'au e aumaia le tamaoaiga o le poto masani i galuega uma.
Fa'amatalaga Auaunaga
| Fa'asologa fa'asologa | Fuafuaga Fa'asologa | Fa'amatalaga fautuaina | Pulea lelei |
| Illumina NovaSeq poʻo DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Manaoga Auaunaga
| Fa'atotonuga (ng/µL) | Aofa'iga (ng) | Volume (µL) |
| ≥1 | ≥30 | ≥20 |
● palapala/palapala: 2-3g
● Intestinal content-manu: 0.5-2g
● Intestinal content-iniseti: 0.1-0.25g
● Lau'ele'ele o la'au (fa'amaoaina palapala): 0.5-1g
● Sua fa'afefeteina fa'atamaoaigaina palapala): 0.2-0.5g
● Faeces (manu tetele): 0.5-2g
● Faeces ( isumu): 3-5grains
● Suavai o le pulmonary alveolar lavage: pepa faamama
● Fufulu fa'atama: 5-6 swabs
● Su'e pa'u/su'u totoga/faua/mea vaivai gutu/su'u fa'a'au/su'u fa'a'au: 2-3 swabs
● Mea fa'aola i luga ole laiga: 5-6 swabs
● Suavai/ea/biofilm: pepa faamama
● Endophytes: 2-3g
● Taofi nifo: 0.5-1g
Auala o Galuega Au'aunaga
Fa'ata'ita'iga tu'uina atu
Fausia faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
E aofia ai le au'ili'iliga nei:
● Fa'asologa o fa'amaumauga lelei
● Metagenome faʻapotopotoga ma faʻasologa o gafa
● Faʻamatalaga o kenera
● Su'esu'ega fa'avasegaga o le alphanomic alpha diversity
● Su'esu'ega fa'atino a le nu'u: galuega fa'aola, metabolic, fa'ama'i fa'ama'i
● Su'esu'ega i tulaga uma e lua o galuega ma fa'aletupe:
Su'esu'ega eseese o le Beta
Su'esu'ega va'aiga
Su'esu'ega fa'atasi: i le va o mea tau le si'osi'omaga ma le tu'ufa'atasiga o le OUT ma le eseesega
Su'esu'ega fa'atino: CARD antibiotic resistance
Su'esu'ega 'ese'ese ole auala ole metabolic KEGG: fa'afanua vevela o auala taua
Alfa 'ese'ese o fa'asoa fa'aletupe: ACE index
Beta eseese o le tufatufaina lafoga: PCoA
Su'esu'e le aga'iga'i i luma na fa'afaigofieina e le BMKGene's metagenome sequencing services ma Illumina e ala i le fa'aputuina o fa'asalalauga.
Hai, Q. et al. (2023) 'Metagenomic ma metabolomic su'esu'ega o suiga i totonu o le intestinal i totonu o le nuanua i'a (Oncorhynchus mykiss) ua a'afia i fa'ama'i pipisi hematopoietic necrosis virus i le vevela vai aganu'u eseese',Tuaoi i Microbiology, 14, itulau. 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. et al. (2023) 'Microbial community, genes resistance, and resistome risks in urban Lakes of different trophic states: Sootaga i totonu ma aafiaga mai fafo',Tusitala o Mea Mata'utia Fa'agasolo, 9, itulau. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Su'esu'ega Metagenomic Fa'aalia Eseesega i le Fa'avasegaina ma le Fa'atinoga i le va o Mea Fa'alaiti e Fa'atatau i le Sua ma le Malosi o le Mamoe Rumen',Tuaoi i Microbiology, 13, itulau. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Obese Ningxiang puaa e maua mai le microbiota toe faʻaogaina le carnitine metabolism e faʻaleleia ai maso gaʻo gaʻo faʻaputu i puaa paʻu DLY',Le Faafouga, 4(5), itulau. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Malamalamaaga Metagenomic i tulaga lamatia o sui o meaola / palasitika e le mafai ona fa'aleagaina ma otaota e le o ni palasitika i le pito i luga ma le pito i lalo o Haihe Estuary, Saina',Saienisi o le Siosiomaga Aofa'i, 887, itulau. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
