MICROBIAL
Bhakitiriya kuenderana uye kusamira ne biochar yakavandudza tebuconazole kuora, ivhu microbiome kuumbwa uye kushanda.
Yakazara-kureba 16S amplicon sequencing |PacBio HiFi |Alpha kusiyana |Beta kusiyana
Muchidzidzo ichi, yakazara-kureba 16S amplicon sequencing nePacBio uye bioinformatic ongororo yakapihwa neBiomarker Technologies.
Pfungwa huru
Biochar immobilized tebuconazole-degrading bacteria Alcaligenes faecalis WZ-2 yakadzidzwa pakubudirira kwebiodegradation uye inokanganisa pavhu rakasvibiswa tebuconazole mukuenzanisa nekusununguka kwekusununguka kunokonzera WZ-2.
1. Biochar-immobilized WZ-2 yakaratidza kukanganisa kwakanyanya mu tebuconazole kana ichienzaniswa nemahara WZ-2 nekuderedza tebuconazole hafu yeupenyu muvhu kubva pamazuva 18.7 kusvika ku13.3 mazuva.
2. Biochar-immobilized WZ-2 yakakwanisa kudzoreredza ivhu rekuzvarwa microbial enzyme mabasa, kusanganisira urease, dehydrogenase uye invertase, nezvimwe.
3. Microbial profile in biochar-immobilized WZ-2 yakabatwa ivhu inotsanangurwa nekuzara-kureba 16S sequencing inotsigirwa zvakasimba kuti iyi system inogona kudzoreredza hutano hwevhu nekuvandudza mabhakitiriya munharaunda pasi pekusvibiswa kwetebuconazole.
Kuedza (kutevedzana kwakabatana)
Kuunganidza: CK: Ivhu remusikirwo;T: Ivhu rakarungwa ne tebuconazole;S: Tebuconazole yaiva nevhu rine mahara strain WZ-2;BC: Tebuconazole yaiva nevhu rine biochar;BCS: Tebuconazole yaiva nevhu rine biochar isingafambiswi WZ-2.
Sampling: Yakazara ivhu DNA kutorwa yakakwidziridzwa ne16S rDNA primers
27F (5′-AGAGTTTGATCCTGGCTCAG-3′) uye 1492R (5′-GGTTACCTTGTTACGA),repesenting yakazara-kureba 16S rDNA
Sequencing chikuva: PacBio RS II
Sequencing zano: CCS HIFI inoverenga
Kuongorora data:BMKCloudBioinformamatic Platforming inoita kuti goldfish ive yakanakisa genetic modhi system yehove physiology uye shanduko.
Mhedzisiro
Ivhu microbial nharaunda chimiro chakatemwa ne16S rDNA kutevedzana.OTU kupfuma uye alpha diversity index, kusanganisira Chao1, Ace, Shannon uye Simpson indices yakaongororwa kuratidza kusiyana kwemarudzi muhurongwa hwega hwega.Mushure memazuva makumi matanhatu ekukura, ma indices akaratidzwa zvakafanana, kureva tebuconazole inogona kuderedza kupfuma kwemarudzi uye kusiyana-siyana muvhu.Nekudaro, nekuwedzera kuomarara WZ-2, nharaunda yemabhakitiriya evhu yakadzoserwa zvishoma maererano nehupfumi uye kusiyana.Musiyano ushoma wakaonekwa pakati peBC, BCS neCK, zvichiratidza kuti biochar uye biochar-immobilized WZ-2 inogona kunyatso kudzorera hutano hwehupenyu hwevhu.
Unweighted Pair-group Method ine Arithmetic Means (UPGMA) yakashandiswa muchidzidzo ichi kuratidza kusiyana kwebeta pakati pemapoka.Sezvinoratidzwa mumufananidzo unotevera, BC, BSC uye CK vakagovera mamwe maitiro akafanana ekugadzirwa kwe microbial zvichienzaniswa neboka T uye S, izvo zvakaratidza zvakare kuti kuiswa kwebiochar mu tebuconazole-yakasvibiswa ivhu bioremediation inogona kunyanya kufambisa kupora kwenharaunda yehutachiona muvhu.
Mufananidzo.UPGMA kusanganisa kwenharaunda yebhakitiriya pa phylum level pasi pekurapwa kwakasiyana
Reference
Zuva, Tong, nevamwe."Kuenderana kwemabhakitiriya uye kusamira nebiochar kwakavandudza tebuconazole, kuumbwa kwevhu uye kushanda."Nyaya Yezvinhu Zvinokuvadza398 (2020): 122941.
Nhau Nezvakakosha ine chinangwa chekugovera nyaya dzichangoburwa dzakabudirira neBiomarker Technologies, kutora zvinyowani zvakabudirira zvesainzi pamwe nemaitiro ane mukurumbira akashandiswa panguva yekudzidza.
Nguva yekutumira: Jan-08-2022