MICROBIAL
Bacterial compatibility thiab immobilization nrog biochar txhim kho tebuconazole degradation, av microbiome muaj pes tsawg leeg thiab ua haujlwm
Full-ntev 16S amplicon sequencing |PacBio HiFi |Alpha ntau haiv neeg |Beta ntau haiv neeg
Nyob rau hauv txoj kev tshawb no, tag nrho-ntev 16S amplicon sequencing los ntawm PacBio thiab bioinformatic tsom xam yog muab los ntawm Biomarker Technologies.
Qhov tseem ceeb
Biochar immobilized tebuconazole-degrading cov kab mob Alcaligenes faecalis WZ-2 tau kawm txog biodegradation efficiency thiab cuam tshuam rau cov kab mob tebuconazole hauv kev sib piv rau dawb degrading hom WZ-2.
1. Biochar-immobilized WZ-2 tau pom tias muaj kev degradation ntau dua hauv tebuconazole piv rau dawb WZ-2 los ntawm kev txo cov tebuconazole ib nrab-lub neej hauv av los ntawm 18.7 hnub rau 13.3 hnub.
2. Biochar-immobilized WZ-2 muaj peev xwm los kho cov av microbial enzyme kev ua si, suav nrog urease, dehydrogenase thiab invertase, thiab lwm yam.
3. Microbial profile nyob rau hauv biochar-immobilized WZ-2 kho av txiav txim siab los ntawm tag nrho-ntev 16S sequencing txhawb zog tias qhov system no tuaj yeem kho cov av kev noj qab haus huv los ntawm kev txhim kho cov kab mob hauv zej zog raws li kev sib kis ntawm tebuconazole.
Kev sim (Sequencing related)
Grouping: CK: Ntuj av;T: Av spiked nrog tebuconazole;S: Tebuconazole muaj cov av uas tsis muaj kab mob WZ-2;BC: Tebuconazole muaj av nrog biochar;BCS: Tebuconazole muaj cov av nrog biochar immobilized WZ-2.
Sampling: Tag nrho cov av DNA extraction amplified los ntawm 16S rDNA primers
27F (5′-AGAGTTTTGATCTGGCTCAG-3′) thiab 1492R (5′-GGTTACCTTGTTACGA),repesenting puv-ntev 16S rDNA
Sequencing platform: PacBio RS II
Sequencing lub tswv yim: CCS HIFI nyeem
Kev txheeb xyuas cov ntaub ntawv:BMKCloudBioinformatic Platforming ua cov ntses goldfish ua qauv zoo heev rau cov ntses physiology thiab evolution.
Kev tshwm sim
Av microbial cov qauv hauv zej zog tau txiav txim siab los ntawm 16S rDNA sequencing.OTU nplua nuj thiab alpha diversity Performance index, suav nrog Chao1, Ace, Shannon thiab Simpson indices raug soj ntsuam los qhia txog hom kev sib txawv hauv txhua qhov system.Tom qab 60 hnub incubation, tag nrho cov indices qhia zoo sib xws, xws li tebuconazole tuaj yeem ua rau txo qis hauv hom kev nplua nuj thiab ntau haiv neeg hauv av.Txawm li cas los xij, los ntawm kev ntxiv cov kab mob WZ-2, cov kab mob hauv av hauv zej zog tau rov qab ib nrab ntawm kev nplua nuj thiab ntau haiv neeg.Kev txwv qhov sib txawv tau pom ntawm BC, BCS thiab CK, qhia tias biochar thiab biochar-immobilized WZ-2 tuaj yeem kho cov av lom neeg noj qab haus huv.
Unweighted Pair-group Method with Arithmetic Means (UPGMA) tau siv rau hauv txoj kev tshawb no los qhia txog kev sib txawv ntawm beta ntawm cov pab pawg.Raws li pom hauv daim duab hauv qab no, BC, BSC thiab CK tau sib koom ntau cov qauv ntawm microbial muaj pes tsawg leeg piv rau pawg T thiab S, uas tau qhia ntxiv tias kev qhia txog biochar hauv tebuconazole-contaminated av bioremediation tuaj yeem pab txhawb kev rov qab los ntawm microbial zej zog hauv av.
Daim duab.UPGMA pawg ntawm cov kab mob hauv zej zog ntawm phylum qib raws li kev kho mob sib txawv
Kev siv
Sun, Tong, et al."Bacterial compatibility thiab immobilization nrog biochar txhim kho tebuconazole degradation, av microbiome muaj pes tsawg leeg thiab ua haujlwm."Phau ntawv Journal of Hazardous Materials398 (2020): 122941.
Nyeem tag nrho cov ntawv nyeem
Xov xwm thiab qhov tseem ceeb Lub hom phiaj ntawm kev sib koom cov xwm txheej ua tiav tshiab nrog Biomarker Technologies, khaws cov kev tshawb fawb tshiab thiab cov txuj ci tseem ceeb siv thaum kawm.
Post lub sij hawm: Jan-08-2022