MICROBIAL
Ko te hototahitanga huakita me te kore e nekehia me te biochar i pai ake te paheketanga o te tebuconazole, te hanganga microbiome oneone me te mahi.
Te raupapa amplicon 16S roa-katoa |PacBio HiFi |Kanorau Arepa |Te kanorau Beta
I roto i tenei rangahau, ko te raupapa amplicon 16S roa-katoa na PacBio me te tātari bioinformatic i whakaratohia e Biomarker Technologies.
Nga mea nui
Ko te huakita tebuconazole-degrading huakita Alcaligenes faecalis WZ-2 kare i nekehia te koiora i akohia mo te pai o te whakahekenga koiora me te pa ki te oneone pokea tebuconazole hei whakataurite ki te riaka whakaheke kore utu WZ-2.
1. Ko te WZ-2 kua whakakorehia te koiora i whakaatu he pai ake te whakahekenga o te tebuconazole ki te WZ-2 kore utu ma te whakaiti i te haurua o te tebuconazole i roto i te oneone mai i te 18.7 ra ki te 13.3 ra.
2. I taea e te WZ-2 kua whakakorehia te koiora ki te whakahoki mai i nga mahi aa-roto moroiti mo te oneone taketake, tae atu ki te urease, dehydrogenase me te invertase, aha atu.
3. Ko te tohu moroiti i roto i te oneone whakatoi WZ-2 kua kore-koiora i whakatauhia e te raupapatanga 16S roa-katoa e tautoko ana ka taea e tenei punaha te whakaora i te hauora o te oneone ma te whakapai ake i te hanganga hapori huakita i raro i te poke o te tebuconazole.
Whakamatau (Whakaraupapatanga e pa ana)
Whakarōpū: CK: Whenua māori;T: Te oneone kua purua ki te tebuconazole;S: Tebuconazole te oneone kei roto me te riaka kore utu WZ-2;BC: Tebuconazole kei roto te oneone me te biochar;BCS: Tebuconazole te oneone kei roto me te biochar immobilized WZ-2.
Tauira: Tapeke tangohanga DNA oneone kua whakanuia e te 16S rDNA tuatahi
27F (5′-AGAGTTTGATCCTGGCTCAG-3′) me 1492R (5′-GGTTACCTTGTTACGA),te whakamau i te 16S rDNA roa
Paparanga raupapa: PacBio RS II
Rautaki raupapa: Ka panui te CCS HIFI
Te tātari raraunga:BMKCloudHangaia e Bioinformatic Platforming te ika koura hei punaha tauira ira tino pai mo te ahuwhenua me te whanaketanga ika.
Hua
Ko te hanganga hapori microbial oneone i whakatauhia e te raupapa 16S rDNA.Ko te whai rawa o OTU me te taupū kanorau alpha, tae atu ki nga tohu Chao1, Ace, Shannon me Simpson i arotakea hei whakaatu i te kanorau momo o ia punaha.I muri i te 60 ra te heki, he rite nga tohu katoa, ara, ka taea e te tebuconazole te whakaiti i nga momo momo me te rereketanga o te oneone.Heoi, na te taapiri i te riaka WZ-2, i ora ake te hapori huakita oneone i runga i te whai rawa me te kanorau.He iti te rereketanga i kitea i waenga i te BC, BCS me te CK, e tohu ana ka taea e te biochar me te biochar-immobilized WZ-2 te whakaora i te hauora koiora oneone.
Ko te Tikanga Takirua Kore Taumaha me te Tikanga Arite (UPGMA) i whakamahia i roto i tenei rangahau hei whakaatu i te kanorau peta i waenga i nga roopu.E whakaatu ana i te ahua e whai ake nei, ko BC, BSC me CK he rite tonu te ahua o te hanganga moroiti i whakaritea ki te roopu T me te S, i tohu ake ko te whakaurunga o te biochar i roto i te bioremediation oneone tebuconazole ka taea te whakaora i te hapori microbial i roto i te oneone.
Pikitia.Ko te kohinga UPGMA o te hapori huakita i te taumata phylum i raro i nga maimoatanga rereke
Tohutoro
Ra, Tong, et al."Ko te hototahitanga o te huakita me te aukati me te biochar i pai ake te paheketanga o te tebuconazole, te hanganga microbiome oneone me te mahi."Te Tuhituhi o nga Rawa Morearea398 (2020): 122941.
Nga korero me nga mea nui Ko te whainga ki te tiri i nga keehi angitu hou me Biomarker Technologies, te hopu i nga whakatutukitanga putaiao hou me nga tikanga rongonui i whakamahia i te wa o te ako.
Te wa tuku: Hanuere-08-2022