Chithunzi cha MICROBIAL
Kugwirizana kwa mabakiteriya ndi kusasunthika ndi biochar kumapangitsa kuwonongeka kwa tebuconazole, kapangidwe ka microbiome ndi magwiridwe antchito.
Kutalika kwathunthu kwa 16S amplicon sequencing |PacBio HiFi |Mitundu ya Alpha |Beta zosiyanasiyana
Mu phunziro ili, kutsatizana kwa matayala amtundu wa 16S ndi PacBio ndi kusanthula kwa bioinformatic kunaperekedwa ndi Biomarker Technologies.
Mfundo zazikuluzikulu
Biochar immobilized tebuconazole-degrading mabakiteriya Alcaligenes faecalis WZ-2 anawunikidwa pa biodegradation bwino ndipo zimakhudza pa tebuconazole dothi loipitsidwa poyerekezera ndi ufulu wonyozeka kupsyinjika WZ-2.
1. Biochar-immobilized WZ-2 ikuwonetsa kuwonongeka kothandiza kwambiri kwa tebuconazole poyerekeza ndi WZ-2 yaulere mwa kuchepetsa theka la moyo wa tebuconazole m'nthaka kuchokera masiku 18.7 mpaka masiku 13.3.
2. Biochar-immobilized WZ-2 inatha kubwezeretsanso ntchito za michere ya nthaka, kuphatikizapo urease, dehydrogenase ndi invertase, ndi zina zotero.
3. Tizilombo tating'onoting'ono tadothi la biochar-immobilized WZ-2 lomwe limapangidwa ndi ma 16S kutalika kwanthawi zonse zimathandizidwa mwamphamvu kuti dongosololi likhoza kubwezeretsa thanzi lanthaka pokonza dongosolo la mabakiteriya m'dera la tebuconazole.
Zochitika (zokhudzana ndi kutsatizana)
Magulu: CK: Dothi lachilengedwe;T: Nthaka yothira ndi tebuconazole;S: Tebuconazole inali ndi dothi lopanda mphamvu WZ-2;BC: Tebuconazole munali dothi ndi biochar;BCS: Tebuconazole munali dothi lokhala ndi biochar immobilized WZ-2.
Zitsanzo: Dothi lonse la DNA m'zigawo zokulitsidwa ndi 16S rDNA primers
27F (5′-AGAGTTTGATCCTGGCTCAG-3′) ndi 1492R (5′-GGTTACCTTGTTACGA),repesenting kutalika kwa 16S rDNA
Sequencing nsanja: PacBio RS II
Njira yotsatirira: CCS HIFI imawerenga
Kusanthula deta:BMKCloudBioinformamatic Platforming imapangitsa nsomba za golide kukhala njira yabwino kwambiri yopangira ma physiology ndi chisinthiko.
Zotsatira
Dothi la tizilombo tating'onoting'ono tadothi linatsimikiziridwa ndi 16S rDNA sequencing.Kulemera kwa OTU ndi ma alpha diversity index, kuphatikiza Chao1, Ace, Shannon ndi Simpson indices adawunikidwa kuti awulule mitundu yamitundu yosiyanasiyana mudongosolo lililonse.Pambuyo pa makulitsidwe kwa masiku 60, zizindikiro zonse zosonyeza zofanana, mwachitsanzo, tebuconazole zitha kuchepetsa kuchulukana kwa mitundu ndi kusiyanasiyana kwa nthaka.Komabe, powonjezera zovuta za WZ-2, gulu la mabakiteriya am'nthaka linachira pang'ono potengera kulemera komanso kusiyanasiyana.Kusiyana kochepa kunawonedwa pakati pa BC, BCS ndi CK, kusonyeza kuti biochar ndi biochar-immobilized WZ-2 zingathe kubwezeretsa thanzi lachilengedwe la nthaka.
Njira Yopanda Zolemetsa yamagulu Awiri yokhala ndi Arithmetic Means (UPGMA) idagwiritsidwa ntchito mu kafukufukuyu kuwonetsa kusiyanasiyana kwa beta pakati pamagulu.Monga momwe tawonetsera m'chithunzi chotsatirachi, BC, BSC ndi CK adagawana njira yofananira ya tizilombo toyambitsa matenda poyerekeza ndi gulu la T ndi S, zomwe zimasonyezanso kuti kuyambitsa biochar mu nthaka yowonongeka ndi tebuconazole kungathandize kwambiri kuti tizilombo toyambitsa matenda tizilombo toyambitsa matenda tizilombo toyambitsa matenda m'nthaka.
Chithunzi.UPGMA clustering of bacterial community at phylum level pansi pa chithandizo chosiyana
Buku
Sun, Tong, et al."Kugwirizana kwa mabakiteriya ndi kusasunthika ndi biochar kumapangitsa kuwonongeka kwa tebuconazole, kapangidwe ka ma microbiome ndi magwiridwe antchito."Journal of Hazardous Materials398 (2020): 122941.
Nkhani ndi Mfundo Zazikulu ikufuna kugawana milandu yaposachedwa kwambiri ndi Biomarker Technologies, kujambula zomwe zakwaniritsa zasayansi komanso njira zodziwika bwino zomwe zimagwiritsidwa ntchito pophunzira.
Nthawi yotumiza: Jan-08-2022