MICROBIAL
Ndakọrịta nje bacteria na immobilization na biochar kwalitere mbibi tebuconazole, ihe mejupụtara ala microbiome na ịrụ ọrụ.
Usoro amplicon ogologo 16S zuru oke |PacBio HiFi |Alfa di iche iche |Beta iche iche
N'ime ọmụmụ ihe a, 16S amplicon sequencing zuru oke site na PacBio na nyocha bioinformatic bụ Biomarker Teknụzụ nyere.
Isi ihe
Biochar immobilized tebuconazole na-emebi nje bacteria Alcaligenes faecalis WZ-2 ka a mụrụ maka ịrụ ọrụ biodegradation ma na-emetụta ala tebuconazole merutere ma e jiri ya tụnyere WZ-2 na-emebi emebi n'efu.
1. Biochar-immobilized WZ-2 gosipụtara mmebi nke ọma na tebuconazole ma e jiri ya tụnyere WZ-2 n'efu site na ibelata tebuconazole ọkara ndụ n'ime ala site na ụbọchị 18.7 ruo ụbọchị 13.3.
2. Biochar-immobilized WZ-2 nwere ike weghachi ala ala microbial enzyme omume, gụnyere urease, dehydrogenase na invertase, wdg.
3. Microbial profaịlụ na biochar-immobilized WZ-2 mesoo ala kpebisiri ike site zuru-ogologo 16S sequencing na-akwado ike na usoro a nwere ike iweghachi ala ahụ ike site melite nje obodo Ọdịdị n'okpuru tebuconazole mmetọ.
Nnwale (metụtara usoro)
Nchịkọta: CK: Ala eke;T: Ala spiked na tebuconazole;S: Tebuconazole nwere ala nwere nsogbu n'efu WZ-2;BC: Tebuconazole nwere ala nwere biochar;BCS: Tebuconazole nwere ala nwere biochar immobilized WZ-2.
Nlele: Ngụkọta mmịpụta DNA nke ala nke 16S rDNA primers kwalitere
27F (5′-AGAGTTTGATCCTGCTCAG-3′) na 1492R (5′-GGTTACCTTGTACGA),na-emegharị ogologo 16S rDNA zuru oke
Usoro nhazi usoro: PacBio RS II
Usoro usoro: CCS HIFI na-agụ
Nyocha data:BMKCloudBioinformatic Platforming na-eme ka azụ̀zụ ọla edo bụrụ ezigbo usoro ihe atụ mkpụrụ ndụ ihe nketa maka physiology azụ na evolushọn.
Nsonaazụ
Ekpebiri nhazi obodo microbial nke ala site na usoro 16S rDNA.Atụlela ụbara OTU na alfa diversity index, gụnyere Chao1, Ace, Shannon na Simpson indices iji kpughee ụdị dị iche iche dị na sistemụ ọ bụla.Mgbe ụbọchị iri isii gachara, egosiputara ụdịrị usoro ahụ, ya bụ tebuconazole nwere ike bute mbelata ụdị akụ na ụba dị iche iche na ala.Agbanyeghị, site n'ịgbakwunye ụdị WZ-2, obodo nje bacteria na-enwetaghachi akụkụ ụfọdụ n'ihe gbasara ma ụbara na ụdị dị iche iche.Ahụrụ oke oke n'etiti BC, BCS na CK, na-egosi na biochar na biochar-immobilized WZ-2 nwere ike weghachi ahụike ahụike nke ala nke ọma.
Ejiri usoro otu ụzọ abụọ na-enweghị oke na ụzọ Arithmetic (UPGMA) tinye n'ọrụ n'ọmụmụ ihe a iji gosi ụdị beta dị iche iche n'etiti otu.Dịka e gosiri na ọnụ ọgụgụ na-esonụ, BC, BSC na CK na-ekekọrịta ụkpụrụ nke microbial dị iche iche ma e jiri ya tụnyere otu T na S, nke gosikwara na iwebata biochar na tebuconazole-emetọ ala bioremediation nwere ike ime ka mgbake nke microbial obodo na ala.
Onyonyo.Nchịkọta UPGMA nke obodo nje na ọkwa phylum n'okpuru ọgwụgwọ dị iche iche
Ntụaka
Sun, Tong, et al."Ndakọrịta nje bacteria na immobilization na biochar kwalitere mmebi tebuconazole, ihe mejupụtara ala microbiome na ịrụ ọrụ."Akwụkwọ akụkọ nke ihe ndị dị ize ndụ398 (2020): 122941.
Akụkọ na isi ihe Ebumnuche iji Biomarker Teknụzụ kekọrịta ikpe kachasị ọhụrụ na-aga nke ọma, na-ewepụta mmezu sayensị ọhụụ yana usoro ama ama etinyere n'oge ọmụmụ ihe ahụ.
Oge nzipu: Jan-08-2022