Nanopore-based Full-length Transcriptome Sequencing
Nanopore sequencing e ikhetholla ho li-platform tse ling tsa ho latellana, ka hore li-nucleotide li baloa ka ho toba ntle le motsoako oa DNA 'me li hlahisa nako e telele ho li-kilobase tse mashome.Sena se matlafatsa ho bala ka ho otloloha ha lingoliloeng tsa bolelele bo felletseng le ho sebetsana le mathata a lithuto tsa transcriptomic.
√ Leeme le ikhethileng la tatelano e itseng
√ Palo e felletseng ea cDNA ho ea ho lithuto tsa sebopeho sa liphatsa tsa lefutso
√ Lintlha tse fokolang tse hlokahalang ho koahela palo e tšoanang ea lingoloa
√ Ho tsebahatsa li-isoform tse ngata ka jini
√ Tlhaloso ea polelo ho boemo ba isoform
Pipeline e tloaelehileng ea Bioinformatics
Linyeoe tse Atlehileng Haufinyane ka Biomarker Technologies
1. Litlhahlobo tse bapisoang tsa li-transcriptomes tsa bolelele bo felletseng li senola Gnetum luofuense stem developmental dynamics.
Journal: Frontiers in Genetics
E phatlalalitsoe: Mar. 2021
Mantsoe a bohlokoa: MinION |Alternative splicing |APA |lncRNA |Litlhaku tse hlahisitsoeng ka mokhoa o fapaneng
Bala Mongolo o felletseng
2. Motsamao o le mong oa li-RNA tse nyane ho tloha ho letlobo ho isa metsong ho li-interspecific heterografts
Journal: Limela tsa Tlhaho
E phatlalalitsoe: Pherekhong 2021
Mantsoe a bohlokoa: Nanopore |Ilumina |phumano ea mRNA ea bolelele bo felletseng
3. Tlhahlobo e feletseng ea transcriptome ea metso ea asparagus e senola mokhoa oa molek'hule oa mamello ea letsoai o bakoang ke arbuscular mycorrhizal fungus.
Journal: Botany ea Tikoloho le Teko
E phatlalalitsoe: Pherekhong 2021
Mantsoe a bohlokoa: MinION |Lingoloa tse hlahisitsoeng ka mokhoa o fapaneng |Lintlha tsa mongolo |Alternative splicing
4. Liphetoho tsa Sebopeho sa 'Mele le Phetolelo ea Bolelele bo Feletseng ba Rose (Rosa chinensis) Metso le Makhasi ho Araba Khatello ea Komello.
Journal: Semela le Cell Physiology
E phatlalalitsoe: Oct. 2020
Mantsoe a bohlokoa: PromethION |Lingoloa tse hlahisitsoeng ka mokhoa o fapaneng |Boprofeta bo sebetsang |Li-isoform tse arohaneng |TF le lncRNA
5. Tlhahlobo le papiso e felletseng ea PacBio le tatellano ea RNA e thehiloeng ho nanopore ea Arabidopsis transcriptome
Journal: Mokhoa oa Semela
E phatlalalitsoe: Mmesa 2020
Mantsoe a bohlokoa: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Khokahano ea liphatsa tsa lefutso |Boitsebiso ba sengoloa |Alternative splicing |SSR |LNCRNA |Isoform quantification
Litaba le Lintlha-khōlō e ikemiselitse ho arolelana linyeoe tsa morao-rao tse atlehileng le Biomarker Technologies, ho hapa likatleho tse ncha tsa mahlale hammoho le mekhoa e tsebahalang e sebelisitsoeng nakong ea thuto.
Nako ea poso: Jan-08-2022