Nanopore-based Yakazara-kureba Transcriptome Sequencing
Nanopore sequencing inozvisiyanisa kubva kune mamwe mapuratifomu ekutevedzana, pakuti nucleotides inoverengwa zvakananga pasina DNA synthesis uye inogadzira kuverenga kwenguva refu pamakumi e kilobases.Izvi zvinopa simba rakananga kuverenga-kunze kweazere-kureba zvinyorwa uye kugadzirisa zvinonetsa muzvidzidzo zvezvinyorwa.
√ Yakaderera kutevedzana-chaiwo kurerekera
√ Yakazara-kureba kuverenga kwecDNA kune yemajini maitiro ezvidzidzo
√ Dhata shoma inodiwa kuvhara nhamba imwechete yezvinyorwa
√ Kuzivikanwa kweakawanda isoforms pajini
√ Kuratidzira kwehuwandu pane isoform-level
Yakajairika Bioinformatics Pipeline
Dzazvino Dzakabudirira Nyaya neBiomarker Technologies
1. Ongororo yekuenzanisa yezvinyorwa zvehurefu hwakazara zvinoratidza Gnetum luofuense stem development dynamics.
Journal: Frontiers muGenetics
Rakabudiswa: Kurume 2021
Keywords: MinION |Alternative splicing |APA |lncRNA |Zvinyorwa zvinotaridzwa zvakasiyana
Verenga zvizere-zvinyorwa
2. Unidirectional kufamba kwemaRNA madiki kubva panhungirwa kuenda kumidzi mune interspecific heterografts
Nyaya: Zvisikwa Zvirimwa
Rakabudiswa: Ndira 2021
Keywords: Nanopore |Ilumina |hurefu hwakazara mbozha mRNA yekuona
3. Yakazara-yakareba transcriptome kuongororwa kwemidzi yeasparagus inoratidza iyo molecular nzira yekushivirira kwemunyu kunokonzerwa ne arbuscular mycorrhizal fungi.
Journal: Environmental uye Yekuedza Botany
Rakabudiswa: Ndira 2021
Keywords: MinION |Zvinyorwa zvakasiyana-siyana |Transcription factors |Alternative splicing
4. Physiological Characteristic Shanduko uye Yakazara-Kureba Transcriptome yeRose (Rosa chinensis) Midzi uye Mashizha muKupindura Kusanaya Stress.
Journal: Plant uye Cell Physiology
Rakabudiswa: Gumiguru 2020
Keywords: PromethION |Zvinyorwa zvakasiyana-siyana |Functional prediction |Spliced isoforms |TF uye lncRNA
5. Kuongorora uye kuenzaniswa kwakazara kwePacBio uye nanopore-based RNA sequencing yeArabidopsis transcriptome.
Journal: Plant Method
Rakabudiswa: Kubvumbi 2020
Keywords: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Genome alignment |Transcript identification |Alternative splicing |SSR |LncRNA |Isoform quantification
Nhau Nezvakakosha inovavarira kugovera nyaya dzichangoburwa dzakabudirira neBiomarker Technologies, kutora budiriro yesainzi pamwe nemaitiro ane mukurumbira akashandiswa panguva yekudzidza.
Nguva yekutumira: Jan-08-2022