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Nanopore sequencing distinguishes itself from other sequencing platforms, in that the nucleotides are read directly without DNA synthesis and generates long reads at tens of kilobases. This empowers direct read-out of full-length transcripts and tackling the challenges in transcriptomic studies.

√ Low sequence-specific bias

√ Full-length readout of cDNA to for gene structure studies

√ Less data required to cover same number of transcripts

√ Identification of multiple isoforms per gene

√ Expression quantification on isoform-level