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ʻO ka hoʻopaʻa ʻana o ka Transcriptome Ka lōʻihi o Nanopore

Hoʻokaʻawale ʻo Nanopore sequencing iā ia iho mai nā pae hoʻonohonoho ʻē aʻe, no ka mea, heluhelu pololei ʻia nā nucleotides me ka ʻole o ka DNA synthesis a hoʻopuka i nā heluhelu lōʻihi ma nā ʻumi kilobases.Hāʻawi kēia i ka heluhelu pololei ʻana i nā transcript lōʻihi a me ka hoʻoponopono ʻana i nā pilikia i nā haʻawina transcriptomic.

√ Kūlana haʻahaʻa kikoʻī kikoʻī

√ Heluhelu lōʻihi o ka cDNA no ka hoʻopaʻa ʻana i ke ʻano gene

√ ʻOi aku ka liʻiliʻi o ka ʻikepili no ka uhi ʻana i ka helu like o nā kope

√ Ka ʻike ʻana o nā isoform he nui no kēlā me kēia gene

√ Ka helu hōʻike ma ka pae isoform

nanopore-1

Pipeline Bioinformatics maʻamau

Ka lōʻihi-trans-bix-1024x254

Nā Hana Pōmaikaʻi Hou loa me Biomarker Technologies

1. Hōʻike nā loiloi hoʻohālikelike o nā transcriptomes holoʻokoʻa i ka hoʻomohala ʻana o Gnetum luofuense stem.

Nūpepa: Frontiers in Genetics

Paʻi ʻia: Mar. 2021

Hua'ōlelo: MinION |Hoʻopili ʻokoʻa |APA |lncRNA |Nā palapala i hōʻike ʻokoʻa

Heluhelu i ka kikokikona piha

2. Ka neʻe unidirectional o nā RNA liʻiliʻi mai nā ʻōpuʻu a i nā aʻa i nā heterografts interspecific

Nūpepa: Nature Plants

Paʻi ʻia: Ian. 2021

Hua'ōlelo: Nanopore |Ilumina |ʻike mRNA paʻa lima piha

3. Hōʻike ka loiloi transcriptome holoʻokoʻa o nā aʻa asparagus i ke ʻano molekala o ka hoʻomanawanui paʻakai i hoʻoulu ʻia e nā fungi mycorrhizal arbuscular.

Nūpepa: Environmental and Experimental Botany

Paʻi ʻia: Ian. 2021

Hua'ōlelo: MinION |Nā palapala i hōʻike ʻokoʻa |Nā kumu unuhi |Hoʻopili ʻokoʻa

4. Nā hoʻololi ʻano kino a me ka Transcriptome lōʻihi piha o nā aʻa a me nā lau o Rose (Rosa chinensis) i pane i ka pilikia maloʻo.

Nūpepa: Plant and Cell Physiology

Hoʻopuka ʻia: ʻOkakopa 2020

Hua'ōlelo: PromethION |Nā palapala i hōʻike ʻokoʻa |wānana hana |Nā isoform i hoʻopili ʻia |TF a me lncRNA

5. Ka nānā 'ana a me ka ho'ohālikelike piha 'ana o PacBio a me ka nanopore-based RNA sequencing o ka Arabidopsis transcriptome.

Nupepa: Mea kanu

Paʻi ʻia: ʻApelila 2020

Hua'ōlelo: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Hoʻoponopono Genome |Hōʻike palapala kope |Hoʻopili ʻokoʻa |SSR |LncRNA |Isoform quantification

Nūhou a me nā mea nui manaʻo e kaʻana like i nā hihia kūleʻa hou loa me Biomarker Technologies, ka hopu ʻana i nā hoʻokō ʻepekema hou a me nā ʻenehana kaulana i hoʻohana ʻia i ka wā o ke aʻo ʻana.


Ka manawa hoʻouna: Jan-08-2022

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