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Single Cell Sequencing Service
, Ngs
, Expression Analysis
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Small RNA sequencing – Illumina – Biomarker Detail:
Service Advantages
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Contamination |
Amount |
OD260/280≥1.7-2.5; OD260/230≥0.5-2.5; |
For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation |
Limited or no protein or DNA contamination shown on gel. |
Conc. ≥300 ng/μl; Volumn ≥ 10 μl; Total ≥ 3 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.
Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
"Sincerity, Innovation, Rigorousness, and Efficiency" is the persistent conception of our firm to the long-term to develop together with consumers for mutual reciprocity and mutual advantage for
Factory Promotional Next Generation Sequencing Provider -
Small RNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Zambia
, Danish
, Las Vegas
, We believe in establishing healthy customer relationships and positive interaction for business. Close cooperation with our customers has helped us to create strong supply chains and reap benefits. Our products have gained us widespread acceptance and the satisfaction of our worldwide valued clients.