● Multiple sequencing strategies available for different research goals
● Bacteria complete genome with 0 Gap guaranteed.
● Highly experienced in bacteria genome assembly with over 10,000 complete genome assembled.
● Professional after-sale technical support team fulfilling more specific research needs.
Sequencing Strategy |
Library |
Quality guaranteed |
Estimated turn-around time |
Nanopore 100X + Illumina 50X |
Nanopore 20K PE150 |
0 Gap |
30 Days |
PacBio HiFi 30X |
PacBio 10K |
● Raw data quality control
● Genome assembly
● Genome component analysis
● Gene function annotation
● Comparative genomic analysis
For DNA extracts:
Sample Type |
Amount |
Concentration |
Purity |
DNA extracts |
> 2 μg |
> 20 ng/μl |
OD260/280= 1.6-2.5 |
For tissue samples:
Sample type | Recommended sample treatment | Sample storage and shipment |
Bacteria | Observe bacteria under microscope and collect bacteria in their exponential phase
Transfer the bacteria culture (containing approx. 3-4.5e9 cells) into a 1.5 or 2 ml eppendorf. (Keep on ice) Centrifuge the tube for 1 min at 14000 g to collect bacteria and remove the supernatant carefully Seal the tube and freeze the bacteria in liquid nitrogen for at least 30 min. Store the tube in -80 ℃ fridge. |
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required. |
1.Circos of bacteria genome
2.Coding gene prediction
3.Comparative genomics analysis: phylogenetic tree