Bacterial complete genome
BMKGene provides a complete bacterial genome assembly service, guaranteeing 0 gaps. This is made possible by the integration of long-read sequencing technologies Nanopore and PacBio for assembly and short-read sequencing with Illumina for assembly validation and error correction of ONT reads. Our service provides the complete bioinformatic workflow from assembly, functional annotation, and advanced bioinformatic analysis, fulfilling specific research goals. This service enables the development of precise reference genomes for various genetic and genomic studies. Additionally, it forms the basis for applications such as strain optimization, genetic engineering, and microbial technology development, ensuring reliable and gap-free genomic data crucial for advancing scientific insights and biotechnological innovation.
Service Features
● With 2 possible options to choose from depending on the desired degree of genome completeness:
● Draft genome option: short-read sequencing with Illumina NovaSeq PE150.
● Complete 0 gap genome:
● Long read sequencing on Nanopore Promethion P48 or PacBio Revio for genome assembly.
● Short read sequencing on Illumina NovaSeq for genome validation and error correction (Nanopore) or just to generate a draft genome.
Service Advantages
● 0 gap genome guaranteed: due to the integration of Illumina sequencing with long read sequencing (Nanopore or PacBio).
● Complete bioinformatics workflow: including not only genome assembly but also prediction of multiple genomic elements, functional gene annotation, and genome visualisations such as Circos plot.
● Extensive Expertise: with over 10,000 microbial genomes assembled, BMKGENE brings over a decade of experience, a highly skilled analysis team, comprehensive content, and excellent post-sales support.
● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
● Multiple sequencing strategies available: for different research goals and requirements of genome completeness
Service Specifications
|
Service |
Sequencing Strategy |
|
Draft Genome |
Illumina PE150 100x |
|
0 Gap Genome |
Nanopore 100x + Illumina PE150 50x Or Pacbio HiFi 30x + Illumina PE150 50x (optional) |
Sample Requirements:
|
Concentration (ng/µL) |
Total amount (µg) |
Volume (µL) |
OD260/280 |
OD260/230 |
|
|
PacBio |
≥20 |
≥1.2 |
≥20 |
1.7-2.2 |
≥1 |
|
Nanopore |
≥40 |
≥2 |
≥20 |
1.7-2.2 |
≥1 |
|
Illumina |
≥1 |
≥0.06 |
≥20 |
1.7-2.2 |
- |
· Bacteria: ≥3.5x1010 cells
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Includes the following analysis:
● Sequencing data quality control
● Genome assembly
● Genome component analysis: prediction of CDS and multiple genomic elements
● Functional annotation with multiple general databases (GO, KEGG, etc) and advanced databases (CARD, VFDB, etc)
● Genome visualization
Delivery of genome in fasta format and genome annotation file (gff).
Gene annotation – GO
Gene annotation – CAZY carbohydrates
Genome visualization – Circos plot
Explore the advancements facilitated by BMKGene’s bacterial genome assembly services through a curated collection of publications.
Dai, W. et al. (2023) ‘Discovery of Bacteroides uniformis F18-22 as a Safe and Novel Probiotic Bacterium for the Treatment of Ulcerative Colitis from the Healthy Human Colon’, International Journal of Molecular Sciences, 24(19), p. 14669. doi: 10.3390/IJMS241914669/S1.
Kang, Q. et al. (2021) ‘Multidrug-resistant Proteus mirabilis isolates carrying blaOXA-1 and blaNDM-1 from wildlife in China: increasing public health risk’, Integrative Zoology, 16(6), pp. 798–809. doi: 10.1111/1749-4877.12510.
Wang, T. T. et al. (2017) ‘Complete genome sequence of endophyte Bacillus flexus KLBMP 4941 reveals its plant growth promotion mechanism and genetic basis for salt tolerance’, Journal of Biotechnology, 260, pp. 38–41. doi: 10.1016/J.JBIOTEC.2017.09.001.
Wang, X. et al. (2021) ‘Multiple-Replicon Resistance Plasmids of Klebsiella Mediate Extensive Dissemination of Antimicrobial Genes’, Frontiers in Microbiology, 12, p. 754931. doi: 10.3389/FMICB.2021.754931/BIBTEX.
