Bacterial and Fungal Whole Genome Re-Sequencing
Bacterial and fungal whole-genome re-sequencing projects are pivotal for advancing microbial genomics, by enabling the completion and comparison of microbial genomes. This facilitates fermentation engineering, the optimization of industrial processes, and the exploration of secondary metabolism pathways. Furthermore, fungal and bacterial re-sequencing is crucial for understanding environmental adaptation, optimizing strains, and revealing genetic evolution dynamics, with broad implications in medicine, agriculture, and environmental science.
Service Advantages
● Bioinformatic analysis includes variant calling: providing functional insights into the re-sequenced genomes.
● Extensive Expertise: with thousands of microbial re-sequencing projects conducted annually, BMKGENE brings over a decade of experience, a highly skilled analysis team, comprehensive content, and excellent post-sales support.
● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
Service Specifications
|
Sequencing platform |
Sequencing Strategy |
Data recommended |
Quality control |
|
Illumina NovaSeq |
PE150 |
Depth of 100x |
Q30≥85% |
Sample Requirements
|
Concentration (ng/µL) |
Total amount (ng) |
Volume (µL) |
OD260/280 |
|
≥1 |
≥60 |
≥20 |
1.7-2.2 |
Bacteria: ≥1x107 cells
Unicellular fungi: ≥5x106-1x107 cells
Macro fungi: ≥4g
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Includes the following analysis:
- Sequencing data quality control
- Alignment to the reference genome
- Variant calling: SNP and InDel
- Variant annotation
Variant calling: SNP types
Variant calling: InDel length distribution
Explore the advancements facilitated by BMKGene’s microbial genome re-sequencing services through a curated collection of publications.
Jia, Y. et al. (2023) ‘Combining Transcriptome and Whole Genome Re-Sequencing to Screen Disease Resistance Genes for Wheat Dwarf Bunt’, International journal of molecular sciences, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) ‘Ampicillin-controlled glucose metabolism manipulates the transition from tolerance to resistance in bacteria’, Science Advances, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) ‘Aliidiomarina halalkaliphila sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China’, Int. J. Syst. Evol. Microbiol, 72, p. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. and Wang, G.-H. (2024) ‘Genome sequence of Staphylococcus nepalensis ZZ-2023a, isolated from Nasonia vitripennis’, Microbiology Resource Announcements. doi: 10.1128/MRA.00802-23.
