Small RNAs are type of short non-coding RNA with average length of 18-30 nt, including miRNA, siRNA and piRNA. These small RNAs have been massively reported to be involved in various biological processes such as mRNA degradation, translation inhibition, heterochromatin formation, etc. SmallRNA sequencing analysis has been widely applied in studies on animal/plant development, disease, virus, etc. Small RNA sequencing analysis platform consists of standard analysis and advanced data mining. On base of RNA-seq data, standard analysis can achieve miRNA identification and prediction, miRNA target gene prediction, annotation and expression analysis. Advanced analysis enables customized miRNA search and extraction, Venn diagram generation, miRNA and target gene network building.