-
![[Copy] Eukaryotic mRNA Sequencing-NGS](http://www.bmkgene.com/uploads/网站产品25.1.14-033.jpg)
[Copy] Eukaryotic mRNA Sequencing-NGS
mRNA sequencing, a versatile technology, empowers the comprehensive profiling of all mRNA transcripts within cells under specific conditions. With its wide-ranging applications, this cutting-edge tool unveils intricate gene expression profiles, gene structures, and molecular mechanisms associated with diverse biological processes. Widely adopted in fundamental research, clinical diagnostics, and drug development, mRNA sequencing offers insights into the intricacies of cellular dynamics and genetic regulation, sparking curiosity about its potential in various fields.
Platform: Illumina NovaSeq X; DNBSEQ-T7
-
Exosomal mRNA/LncRNA/CircRNA Sequencing-Illumina
Exosomes are small vesicles secreted by cells, typically ranging in diameter from 30 to 100 nanometers. These vesicles contain various RNA. Exosomes are believed to play crucial roles in intercellular communication, immune responses, and disease development, and can be disseminated to other parts of the body through bodily fluids such as plasma, saliva, and urine. They carry specific biomolecules to regulate the functions of recipient cells, influencing cellular physiological states. Exosomes are also considered to play key roles in disease development, including cancers, neurodegenerative diseases, and inflammatory conditions. Research on exosomes offers new insights and methods for the diagnosis, treatment, and prevention of diseases.
-
Exosomal Small RNA Sequencing-Illumina
Exosomes are small vesicles secreted by cells, typically ranging in diameter from 30 to 100 nanometers. These vesicles contain various RNA. Among the RNA types in exosomes, the most common and extensively studied is microRNA (miRNA). miRNA is a class of non-coding small RNAs approximately 18-25 nucleotides in length. They mediate post-transcriptional gene silencing by binding to the 3′ untranslated region (3′ UTR) of target mRNAs, thereby regulating gene expression. For example, exosomes secreted by certain tumor cells contain specific miRNAs, such as miR-126 and miR-92a. These miRNAs can influence gene expression in recipient cells and promote tumor angiogenesis (Tomohiro Umezu, et al., Oncogene, 2012).
-
BMKMANU S3000_Spatial Transcriptome
Spatial transcriptomics stands at the forefront of scientific innovation, empowering researchers to delve into intricate gene expression patterns within tissues while preserving their spatial context. Amidst various platforms, BMKGene has developed the BMKManu S3000 Spatial Transcriptome Chip, boasting an enhanced resolution of 3.5µm, reaching the subcellular range, and enabling multi-level resolution settings. The S3000 chip, featuring approximately 4 million spots, employs microwells layered with beads loaded with spatially barcoded capture probes. A cDNA library, enriched with spatial barcodes, is prepared from the S3000 chip and subsequently sequenced on the Illumina NovaSeq platform. The combination of spatially barcoded samples and UMIs ensures the accuracy and specificity of the data generated. The BMKManu S3000 chip’s is extremely versatile, offering multi-level resolution settings that can be finely tuned to different tissues and desired levels of detail. This adaptability positions the chip as an outstanding choice for diverse spatial transcriptomics studies, ensuring precise spatial clustering with minimal noise. The use of cell segmentation technology with BMKManu S3000 enables the delimitation of transcriptional data to the boundaries of cells, resulting in an analysis that has direct biological meaning. Furthermore, the improved resolution of S3000 results in higher number of genes and UMIs detected per cell, enabling a much more accurate analysis of the spatial transcription patterns and clustering of cells.
-
DNBSEQ pre-made libraries
DNBSEQ, developed by MGI, is an innovative NGS technology that has managed to decrease further down the sequencing costs and increase throughput. Preparation of DNBSEQ libraries involves DNA fragmentation, preparation of ssDNA, and rolling circle amplification to obtain the DNA nanoballs (DNB). These are then loaded onto a solid surface and subsequently sequenced by combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ technology combines the advantages of having a low amplification error rate with using high density error patterns with nanoballs, resulting in sequencing with higher throughput and accuracy.
Our pre-made library sequencing service enables customers to prepare Illumina sequencing libraries from diverse sources (mRNA, whole genome, amplicon, 10x libraries, among others), which are converted to MGI libraries in our laboratories to be sequenced in DNBSEQ-T7, enabling high data amounts at lower costs.
-
Hi-C based Chromatin Interaction
Hi-C is a method designed to capture genomic configuration by combining probing proximity-based interactions and high-throughput sequencing. The method is based on chromatin crosslinking with formaldehyde, followed by digestion and re-ligation in a way that only fragments that are covalently linked will form ligation products. By sequencing these ligation products, it is possible to study the 3D organization of the genome. Hi-C enables studying the distribution of the portions of the genome that are lightly packed (A compartments, euchromatin) and more likely to be transcriptionally active, and the regions that are more tightly packed (B compartments, Heterochromatin). Hi-C can also be used to pinpoint Topologically Associated Domains (TADs), regions of the genome that have folded structures and are likely to have similar expression patterns, and to identify chromatin loops, DNA regions that are anchored together by proteins and that are often enriched in regulatory elements. BMKGene’s Hi-C sequencing service empowers researchers to explore the spatial dimensions of genomics, opening new avenues for understanding genome regulation and its implications in health and disease.
-
TGuide Smart Magnetic Plant RNA Kit
TGuide Smart Magnetic Plant RNA Kit
Purify high-quality total RNA from plant tissues
-
TGuide Smart Blood/Cell/Tissue RNA Kit
TGuide Smart Blood/Cell/Tissue RNA Kit
The prefilled cartridge / plate reagent kit for purification of high-yield, high-purity, high-quality, inhibitor-free total RNA from animal tissue/cell/fresh whole blood
-
TGuide Smart Magnetic Plant DNA Kit
TGuide Smart Magnetic Plant DNA Kit
Purify high-quality genomic DNA from various plant tissues
-
TGuide Smart Soil / Stool DNA Kit
TGuide Smart Soil / Stool DNA Kit
Purifies inhibitor-free DNA of high purity and quality from soil and stool samples
-
TGuide Smart DNA Purification Kit
Recovers high-quality DNA from PCR product or agarose gels.
-
TGuide Smart Blood Genomic DNA Kit
TGuide Smart Blood Genomic DNA Kit
The prefilled cartridge / plate reagent kit for genomic DNA purification from blood and buffy coat
-
TGuide Smart Magnetic Tissue DNA Kit
The prefilled cartridge / plate reagent kit for genomic DNA extraction from animal tissues
