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Long non-coding sequencing-Illumina

Long non-coding RNAs (lncRNAs) are RNAs longer than 200 nucleotides that possess minimal coding potential and are pivotal elements within non-coding RNA. Found in the nucleus and cytoplasm, these RNAs play crucial roles in epigenetic, transcriptional, and post-transcriptional regulation, underscoring their significance in shaping cellular and molecular processes. LncRNA sequencing is a powerful tool in Cell differentiation, Ontogenesis, and Human diseases.

Platform: Illumina NovaSeq

Service Details


Demo Results

Featured Publications

Service Advantages

● Joint analysis mRNA and lncRNA: by combining the quantification of mRNA transcripts with the study of lncRNA and their targets it is possible to get an in-depth overview of the regulatory mechanism underlying the cellula response.

● Extensive Expertise: with a track record of processing over 20,000 samples at BMK, spanning diverse sample types and lncRNA projects, our team brings a wealth of experience to every project.

● Rigorous Quality Control: we implement core control points across all stages, from sample and library preparation to sequencing and bioinformatics. This meticulous monitoring ensures the delivery of consistently high-quality results.

● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.

Sample Requirements and Delivery



Recommended data

Data QC

rRNA depleted directional library

Illumina PE150

10-16 Gb




Amount (μg)



≥ 80

≥ 0.5



Limited or no protein or DNA contamination shown on gel.

For plants: RIN≥6.5;

For animals: RIN≥7.0;


limited or no baseline elevation

● Plants:

  Root, Stem or Petal: 450 mg

  Leaf or Seed: 300 mg

  Fruit: 1.2 g

● Animal:

   Heart or Intestine: 450 mg

   Viscera or Brain: 240 mg

   Muscle: 600 mg

   Bones, Hair or Skin: 1.5g

● Arthropods:

   Insects: 9g

   Crustacea: 450 mg

● Whole blood: 2 tubes

● Cells: 106 cells

● Serum and Plasma: 6 mL

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...

1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

Sample QC

Experiment design

sample delivery

Sample delivery

Pilot experiment

RNA extraction

Library Preparation

Library construction



Data analysis

Data analysis

After sale Services

After-sale services

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  • Bioinformatics



    Differential Gene Expression (DEGs) analysis




    Quantification of lncRNA expression – clustering



    Enrichment of lncRNA target genes



    Joint mRNA and lncRNA position analysis – Circos plot (middle circle is mRNA and inner cicrlce is lncRNA)


    Explore the advancements facilitated by BMKGene’s lncRNA equencing services through a curated collection of publications.


    Ji, H. et al. (2020) ‘Identification, functional prediction, and key lncRNA verification of cold stress-related lncRNAs in rats liver’, Scientific Reports 2020 10:1, 10(1), pp. 1–14. doi: 10.1038/s41598-020-57451-7.

    Jia, Z. et al. (2021) ‘Integrative Transcriptomic Analysis Reveals the Immune Mechanism for a CyHV-3-Resistant Common Carp Strain’, Frontiers in Immunology, 12, p. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.

    Wang, X. J. et al. (2022) ‘Multi-Omics Integration-Based Prioritisation of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Molecular Characteristics and Drug Candidates’, Frontiers in Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.

    Xiao, L. et al. (2020) ‘Genetic dissection of the gene coexpression network underlying photosynthesis in Populus’, Plant Biotechnology Journal, 18(4), pp. 1015–1026. doi: 10.1111/PBI.13270.

    Zheng, H. et al. (2022) ‘A Global Regulatory Network for Dysregulated Gene Expression and Abnormal Metabolic Signaling in Immune Cells in the Microenvironment of Graves’ Disease and Hashimoto’s Thyroiditis’, Frontiers in Immunology, 13, p. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.

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