● Comprehensive Analysis: Professional analysis pipeline to meet a variety of customized analysis needs;
● Extensive Experience: Hundreds of projects successfully completed so far,including animal and plant samples.
Platform: NovaSeq 6000 platform, PE150
Library type: 100-500 bp insert DNA library (depends on peak distribution)
Sequencing strategy: Paired-End 150 bp
Recommended data output: 50M reads
Sample type: Tissues, live cells, or frozen cells and so on
Cell number: ≥ 5×105 cells, an accurate cell number is key to success of the experiment;
Tissue weight: ≥ 200mg fresh tissue;
1.Heatmap on ATAC reads distribution at TSS and adjacent regions (±3 kb)
2.Distribution of open chromatin region in different genome components
3.Differential peak calling between groups