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Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

ATAC-seq is a high-throughput sequencing method for analysis of genome-wide chromatin accessibility, which is important for global epigenetic control of gene expression. Sequencing adapters are inserted into open chromatin regions by hyperactive Tn5 transposase. After PCR amplification, a sequencing library is constructed. All the open chromatin regions can be obtained under a specific space-time condition, not only limited to the binding sites of a transcription factor, or a certain histone modified region.


Service Details

Bioinformatics

Demo Results

Service Advantages

●  Comprehensive Analysis: Professional analysis pipeline to meet a variety of customized analysis needs;

●  Extensive Experience: Hundreds of projects successfully completed so far,including animal and plant samples.

Service Specifications

Platform: Illumina NovaSeq platform

Library type: ATAC-seq

Recommended data output: ≥20M reads

Sample Requirements

Sample type: Tissues, live cells, or frozen cells and so on

Cell number: ≥ 5×105 cells, an accurate cell number is key to success of the experiment;

Tissue weight: ≥ 200mg fresh tissue;

Blood: ≥ 2 mL

Service Work Flow

Sample QC

Experiment design

sample delivery

Sample delivery

Pilot experiment

DNA extraction

Library Preparation

Library construction

Sequencing

Sequencing

Data analysis

Data analysis

After sale Services

After-sale services


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  • 表观-02

    1.Heatmap on ATAC reads distribution at TSS and adjacent regions (±3 kb)

    3(1)2.Distribution of open chromatin region in different genome components

    4(1)

     3.Differential peak calling between groups

    5(1)

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