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Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq) Featured Image
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)
  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

ATAC-seq is a high-throughput sequencing method for analysis of genome-wide chromatin accessibility, which is important for global epigenetic control of gene expression. Sequencing adapters are inserted into open chromatin regions by hyperactive Tn5 transposase. After PCR amplification, a sequencing library is constructed. All the open chromatin regions can be obtained under a specific space-time condition, not only limited to the binding sites of a transcription factor, or a certain histone modified region.


Service Details

Bioinformatics

Demo Results

Service Advantages

●  Comprehensive Analysis: Professional analysis pipeline to meet a variety of customized analysis needs;

●  Extensive Experience: Hundreds of projects successfully completed so far，including animal and plant samples.

Service Specifications

Platform: NovaSeq 6000 platform, PE150

Library type: 100-500 bp insert DNA library (depends on peak distribution)

Sequencing strategy: Paired-End 150 bp

Recommended data output: 50M reads

Sample Requirements

Sample type: Tissues, live cells, or frozen cells and so on

Cell number: ≥ 5×105 cells, an accurate cell number is key to success of the experiment;

Tissue weight: ≥ 200mg fresh tissue;

Service Work Flow

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Experiment design

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Sample delivery

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DNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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    • 表观-02

    1.Heatmap on ATAC reads distribution at TSS and adjacent regions (±3 kb)

    3（1）2.Distribution of open chromatin region in different genome components

    4（1）

     3.Differential peak calling between groups

    5（1）

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