Ø Highly Experienced: Over 200,000 samples have been processed in BMK covering diverse sample types, including cell culture, tissue, body fluid, etc. and over 7,000 mRNA-Seq projects closed covering various research area.
Ø Strict quality control system: Core quality control points through all steps including sample preparation, library preparation, sequencing and bioinformatics are under close monitoring in order to deliver high-quality results.
Ø Multiple databases available for function annotation and enrichment studies to fulfill diverse research goals.
Ø After-sale services: After-sale services valid for 3 months upon project completion, including projects follow-up, trouble-shooting, results Q&A, etc.
|Library||Sequencing strategy||Data recommended||Quality Control|
|Poly A enriched||Illumina PE150||
|OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel.||For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation||Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg|
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...
Eukaryotic mRNA sequencing analysis workflow
Ø Raw data quality control
Ø Reference genome alignment
Ø Transcript structure analysis
Ø Expression quantification
Ø Diffferential expression analysis
Ø Function annotation and enrichment
1.mRNA Data Saturation curve
2.Differential expression analysis-Volcano plot
3.KEGG annotation on DEGs
4.GO classification on DEGs