Fungal Genome
Service Advantages
● Multiple sequencing strategies available for different research goals
● Highly experienced in fungi genome assembly with over 10,000 pene-complete genome assembled.
● Professional after-sale technical support team fulfilling more specific research needs.
Service Specifications
|
Service |
Sequencing Strategy |
Quality guaranteed |
Estimated turn-around time |
|
Fungal fine map |
Illumina 50X+Nanopore 100X |
Contig N50≥2 Mb |
35 working days |
|
PacBio HiFi 30X |
|||
|
Fungal pene-complete map |
Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X |
Chromosome anchoring ratio >90% |
45 working days |
Bioinformatics analyses
● Raw data quality control
● Genome assembly
● Genome component analysis
● Gene function annotation
● Comparative genomic analysis
Sample Requirements and Delivery
Sample Requirements:
For DNA extracts:
|
Sample Type |
Amount |
Concentration |
Purity |
|
DNA extracts |
＞ 1.2 μg |
＞ 20 ng/μl |
OD260/280= 1.6-2.5 |
For tissue samples:
| Sample type | Recommended sample treatment | Sample storage and shipment |
| Unicellular Fungus | Observe yeast under microscope and collect them in their exponential phase
Transfer the culture (containing approx. 3-4.5e9 cells) into a 1.5 or 2 ml eppendorf. (Keep on ice) Centrifuge the tube for 1 min at 14000 g to collect bacteria and remove the supernatant carefully Seal the tube and freeze the bacteria in liquid nitrogen for at least 1-3 h. Store the tube in -80 ℃ fridge. |
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required. |
| Macro Fungus | Tissue in actively growing phase is recommended.
Rinse the tissue with endotoxin-free water, then 70% ethonal. Save the sample in cryo-tubes. |
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
1.Circos diagram on fungal genomic components
2.Comparative genomics analysis: Phylogenetic tree
