Fungal Genome

Biomarker Technologies provide genome survey, fine genome and pene-complete genome of fungal depending on specific research goal. Genome sequencing, assembly and functional annotation can be achieved by combining Next-generation sequencing + Third generation sequencing to achieve high-level genome assembly. Hi-C technology can also be employed to facilitate genome assembly at chromosome level.

Platform：PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq 6000

Service Details

Demo Results

Service Advantages

● Multiple sequencing strategies available for different research goals

● Highly experienced in bacteria genome assembly with over 10,000 complete genome assembled.

● Professional after-sale technical support team fulfilling more specific research needs.

Service Specifications

Service	Sequencing Strategy	Quality guaranteed	Estimated turn-around time
Fungal fine map	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	35 working days
Fungal fine map	PacBio HiFi 30X	Contig N50≥2 Mb	35 working days
Fungal pene-complete map	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Chromosome anchoring ratio >90%	45 working days

Bioinformatics analyses

● Raw data quality control

● Genome assembly

● Genome component analysis

● Gene function annotation

● Comparative genomic analysis

Sample Requirements and Delivery

Sample Requirements:

For DNA extracts:

Sample Type	Amount	Concentration	Purity
DNA extracts	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

For tissue samples:

Sample type

Recommended sample treatment

Sample storage and shipment

Unicellular Fungus

Observe yeast under microscope and collect them in their exponential phase

Transfer the culture (containing approx. 3-4.5e9 cells) into a 1.5 or 2 ml eppendorf. (Keep on ice)

Centrifuge the tube for 1 min at 14000 g to collect bacteria and remove the supernatant carefully

Seal the tube and freeze the bacteria in liquid nitrogen for at least 1-3 h. Store the tube in -80 ℃ fridge.

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Macro Fungus

Tissue in actively growing phase is recommended.

Rinse the tissue with endotoxin-free water, then 70% ethonal.

Save the sample in cryo-tubes.