10x Genomics Visium Spatial Transcriptome
Service Advantages
1) One-stop service integrates all experience and skill based steps, including cryo-sectioning, HE staining, tissue optimization, spatial barcoding, library preparation, sequencing and bioinformatics
2) Highly skilled technical group with experience in over 250 tissue types, 50+ species including human, mouse, mammal, fish and plants
3) Real-time update on entire project with full control of experimental progress
4) Comprehensive standard bioinformatics package with 29 analyses, 100+ high-qulity figures
5) Customized data analysis and visualization available for different research requests
Service Specifications
|
Library |
Sequencing strategy |
Data recommended |
Sample Requirements |
|
10X Visium cDNA library |
10x Visium-Illumina PE150 |
60 Gb/sample |
OCT embedded cryo samples; FFPE samples (Optimal diameter: approx. 6x6x6 mm3) |
For more details on sample preparation guidance and service workflow, please feel free to talk to a BMKGENE expert
Service Work Flow
Experiment design
Sample delivery
Pre-experiment
Spatial barcoding
Library construction
Sequencing
Data analysis
After-sale services
1) Data Quality Control
Data output and quality score distribution
Gene detection per spot
Tissue coverage
2) Inner-sample Analysis
Gene detection
Spot clustering
Differential analysis among clusters
3) Inter-group Analysis
Data combination
Spot clustering of joint data
Differential analysis between groups
1.Spot clustering
2.Spatial distribution of marker genes
3.Marker expression abundance clustering heatmap
4.Inter-sample data analysis
