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10x Genomics Visium Spatial Transcriptome

Visium Spatial Gene Expression is a mainstream spatial transcriptome sequencing technology for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context to discover novel insights into normal development, disease pathology, and clinical translational research. BMKGENE provides comprehensive, one-stop spatial transcriptome sequencing service from samples to biological insights.

Spatial transcriptomics technologies empowered novel perspectives in diverse research arena by resolving gene expression profile with spatial content in heterogenous samples.

Spatial transcriptome chip: 10x Genomics Visium

Platform: Illumina NovaSeq 6000


  • FOB Price: US $0.5 - 9,999 / Piece
  • Min.Order Quantity: 100 Piece/Pieces
  • Supply Ability: 10000 Piece/Pieces per Month
  • Service Details

    Bioinformatics

    Demo Results

    Service Advantages

    1) One-stop service integrates all experience and skill based steps, including cryo-sectioning, HE staining, tissue optimization, spatial barcoding, library preparation, sequencing and bioinformatics

    2) Highly skilled technical group with experience in over 250 tissue types, 50+ species including human, mouse, mammal, fish and plants

    3) Real-time update on entire project with full control of experimental progress

    4) Comprehensive standard bioinformatics package with 29 analyses, 100+ high-qulity figures

    5) Customized data analysis and visualization available for different research requests

    Service Specifications

    Library

    Sequencing strategy

    Data recommended

    Sample Requirements

    10X Visium cDNA library

    10x Visium-Illumina PE150

    60 Gb/sample

    OCT embedded cryo samples;

    FFPE samples 

    (Optimal diameter: approx. 6x6x6 mm3)

    For more details on sample preparation guidance and service workflow, please feel free to talk to a 

    Service Work Flow

    10x (3)

    Experiment design

    10x (4)

    Sample delivery

    10x (2)

    Pre-experiment

    logo

    Spatial barcoding

    10x (5)

    Library construction

    10x (6)

    Sequencing

    10x (7)

    Data analysis

    10x (8)

    After-sale services


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  • 10x (9)

    1) Data Quality Control
    Data output and quality score distribution
    Gene detection per spot
    Tissue coverage

    2) Inner-sample Analysis
    Gene detection
    Spot clustering
    Differential analysis among clusters

    3) Inter-group Analysis
    Data combination
    Spot clustering of joint data
    Differential analysis between groups

     

    1.Spot clustering

    10x (10)2.Spatial distribution of marker genes

    10x (11)3.Marker expression abundance clustering heatmap

    10x (12)4.Inter-sample data analysis

    10x (13)

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