Extensive experience in genome sequencing for over 1000 species.
Over 800 published cases with an accumulative impact factor of over 4000.
Comprehensive bioinformatics analysis on variation calling and function analysis.
Platform |
Library |
Recommended seq depth |
|
Illumina |
PE 150 |
For SNP, InDel calling ≥ 10x For SV, CNY calling ≥ 30x
|
|
Nanopore
|
8 kb |
For SV, CNY calling ≥ 20x |
|
Pacbio |
CCS |
15 kb |
For SNP, InDel, SV, CNY calling ≥ 10x |
Platform |
Conc.(ng/μL)
|
Amount (ng)
|
Purity
|
Agarose gel
|
||
OD260/280 |
OD260/230 |
1. Clear main band with no or limited degradation observed on gel. 2. No or limited RNA or protein contamination
|
||||
Illumina |
≥1 |
≥30
|
- |
- |
||
Nanopore
|
≥30 |
Depends on data yield 10μg/cell |
1.7-2.2 |
≥1.5 |
||
Pacbio |
CCS |
≥50 |
1.7-2.2 |
1.8-2.5 |
1) Statistics of Genome Mapping
Table 1 Statistics of mapping result
Figure 1 Distribution of insert size and reads coverage.
2) Variate Detection
Figure 2 Statistics and annotation of SNP/INDEL/SV among Samples
Figure 3 Genome-wide distribution of vatiations
3) Functional annotation of variations
2019 |
Nature communications |
Whole-genome resequencing reveals Brassica napus origin and genetic loci involved in its improvement |
2020 |
PNAS |
The evolutionary origin and domestication history of goldfish (Carassius auratus) |
2021 |
Plant Biotechnology Journal |
Reference genomes of the two cultivated jute species |
2021 |
Plant Biotechnology Journal |
Genomic signatures of vegetable and oilseed allopolyploid Brassicajuncea and genetic loci controlling the accumulation of glucosinolates |
2019 |
Molecular Plant |
Whole-genome resequencing of a world-wide collection of rapeseed accessions reveals genetic basis of their ecotype divergence |
2022 |
Horticulture Research |
Genome-wide association analysis provides molecular insights into the natural variation of watermelon seed size |
2021 |
Journal of Experimental Botany |
Genome-wide association study identifies variants of GhSAD1 conferring cold tolerance in cotton |
2021 |
Journal of Experimental Botany |
Genome-wide association study and transcriptome comparison reveal novel QTL and candidate genes that control petal size in rapeseed |