BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Advantages of BMKMANU S1000
1) Sub-cellular resolution: Each capture area contained >2 million spatial Barcoded Spots with a diameter of 2.5 µm and a spacing of 5 µm between spot centers, enabling spatial transcriptome analysis with sub-cellular resolution (5 µm).
2) Multi-level resolution analysis: Flexible multi-level analysis ranging from 100 μm to 5 μm to resolve diverse tissue features at optimal resolution.
3) Comprehensive transcriptome profiling: Transcripts captured from the entire tissue slide can be analyzed, without restriction on number of target genes and target area.
Service Specifications
|
Library |
Sequencing strategy |
Data recommended |
Quality Control |
|
S1000 cDNA library |
BMKMANU S1000-Illumina PE150 |
60 Gb/sample |
OCT embedded cryo samples; (Optimal diameter: approx. 6x6x6 mm3) |
For more details on sample preparation guidance and service workflow, please feel free to talk to a BMKGENE expert
Service Work Flow
Experiment design
Sample delivery
Pre-experiment
Spatial barcoding
Library construction
Sequencing
Data analysis
After-sale services
The data generated by BMKMANU S1000 is analyzed using the software “BSTMatrix”, which is independently designed by BMKGENE, including:
1) Gene expression matrix generation
2) HE image processing
3) Compatible with downstream third-party software for analysis
4) Online “BSTViewer” helps to obtain visualization results at different resolutions.
1.Spot clustering
2.Spatial distribution
Note: Resolution level=13 (100 µm, left); 7 (50 µm, right)
3.Marker expression abundance clustering heatmap
4.Inter-sample data analysis
