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Reduced Representation Bisulfite Sequencing (RRBS)

Reduced Representation Bisulfite Sequencing (RRBS) Featured Image
  • Reduced Representation Bisulfite Sequencing (RRBS)
  • Reduced Representation Bisulfite Sequencing (RRBS)
  • Reduced Representation Bisulfite Sequencing (RRBS)

DNA methylation research has always been a hot topic in disease research, and is closely related to gene expression and pheno-typic traits. RRBS is an accurate, efficient and economical method for DNA methylation research. Enrichment of promoter and CpG island regions by enzymatic cleavage (Msp I), combined with Bisulfite sequencing, provides high resolution DNA methylation detection.

Platform: Illumina NovaSeq 6000


Service Details

Demo Result

Service Specifications

Platform: NovaSeq 6000 platform, PE150

Library type: 200-400bp insert bisulfite treated DNA library

Sequencing strategy: Paired-End 150 bp

Recommend data output: 10 Gb raw data/sample

Sample Requirements

DNA quantity: ≥ 2ug

DNA concentration:≥ 20ng/μl.

Purity: OD260/280 = 1.8 to 2.0 without degradation or RNA contamination

Bioinformatics analysis

流程图 羽4-03

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

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    • a. Reference genome alignment statistics

    Statistics of mapping reads
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    Statistics of sequencing depth and coverage of C site

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    Accumulated coverage

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    Digestion efficiency of each sample

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    b. Methylation site detection

    List of methylation levels at C site

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    BS conversion

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    Methylated cytosine
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    Methylation level distribution
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    c. Methylation map

    Genome-wide
    image12

    Genome element

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    d. Comparison of methylation levels between samples
    image14

    Pairwise Correlation of CG methylation
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    PCA-CG methylation
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    Clustering of CG methylation
    image17

    d. Differentially methylation analysis

    Statistics of DMR

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    DMR length distribution
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    Heat map
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    DMR distribution in targeted region
    image21

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