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Reduced Representation Bisulfite Sequencing (RRBS)

DNA methylation research has always been a hot topic in disease research, and is closely related to gene expression and pheno-typic traits. RRBS is an accurate, efficient and economical method for DNA methylation research. Enrichment of promoter and CpG island regions by enzymatic cleavage (Msp I), combined with Bisulfite sequencing, provides high resolution DNA methylation detection.

Platform: Illumina NovaSeq Platform


Service Details

Demo Result

Service Specifications

Platform: NovaSeq platform, PE150

Library type: 200-400bp insert bisulfite treated DNA library

Sequencing strategy: Paired-End 150 bp

Recommend data output: 10 Gb raw data/sample

Sample Requirements

DNA quantity: ≥ 2ug

DNA concentration:≥ 20ng/μl.

Purity: OD260/280 = 1.8 to 2.0 without degradation or RNA contamination

Bioinformatics analysis

流程图 羽4-03

Service Work Flow

sample delivery

Sample delivery

Library Preparation

Library construction

Sequencing

Sequencing

Data analysis

Data analysis

After sale Services

After-sale services


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  • a. Reference genome alignment statistics

    Statistics of mapping reads
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    Statistics of sequencing depth and coverage of C site

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    Accumulated coverage

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    Digestion efficiency of each sample

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    b. Methylation site detection

    List of methylation levels at C site

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    BS conversion

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    Methylated cytosine
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    Methylation level distribution
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    c. Methylation map

    Genome-wide
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    Genome element

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    d. Comparison of methylation levels between samples
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    Pairwise Correlation of CG methylation
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    PCA-CG methylation
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    Clustering of CG methylation
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    d. Differentially methylation analysis

    Statistics of DMR

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    DMR length distribution
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    Heat map
    image20

    DMR distribution in targeted region
    image21

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