Reduced Representation Bisulfite Sequencing (RRBS)
Service Specifications
Platform: NovaSeq platform, PE150
Library type: 200-400bp insert bisulfite treated DNA library
Sequencing strategy: Paired-End 150 bp
Recommend data output: 10 Gb raw data/sample
Sample Requirements
DNA quantity: ≥ 2ug
DNA concentration:≥ 20ng/μl.
Purity: OD260/280 = 1.8 to 2.0 without degradation or RNA contamination
Bioinformatics analysis
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
a. Reference genome alignment statistics
Statistics of mapping reads
Statistics of sequencing depth and coverage of C site
Accumulated coverage
Digestion efficiency of each sample
b. Methylation site detection
List of methylation levels at C site
BS conversion
Methylated cytosine
Methylation level distribution
c. Methylation map
Genome-wide
Genome element
d. Comparison of methylation levels between samples
Pairwise Correlation of CG methylation
PCA-CG methylation
Clustering of CG methylation
d. Differentially methylation analysis
Statistics of DMR
DMR length distribution
Heat map
DMR distribution in targeted region
