Platform: NovaSeq platform, PE150
Library type: 200-400bp insert bisulfite treated DNA library
Sequencing strategy: Paired-End 150 bp
Recommend data output: 10 Gb raw data/sample
DNA quantity: ≥ 2ug
DNA concentration:≥ 20ng/μl.
Purity: OD260/280 = 1.8 to 2.0 without degradation or RNA contamination
a. Reference genome alignment statistics
Statistics of sequencing depth and coverage of C site
Accumulated coverage
Digestion efficiency of each sample
b. Methylation site detection
List of methylation levels at C site
BS conversion
Methylation level distribution
c. Methylation map
Genome element
d. Comparison of methylation levels between samples
Pairwise Correlation of CG methylation
d. Differentially methylation analysis
Statistics of DMR