Platform |
Sequencing Mode |
Applications |
Illumina Nova system |
SE50 PE50 PE150 PE250 |
1. Rapidly sequence whole genomes 2. Zoom in to deeply sequence target regions 3. Utilize RNA sequencing (RNA-Seq) to discover novel RNA variants and splice sites, or quantify mRNAs for gene expression analysis 4. Analyze epigenetic factors such as genome-wide DNA methylation and DNA-protein interactions 5. Sequence cancer samples to study rare somatic variants, tumor subclones, and more Study microbial diversity in humans or in the environment |
● Extensive experience on Illumina sequencing platform with thousands of closed projects with various species.
● Mature sequencing process with short turn-around time.
● Complete downstream bioinformatics analysis services.
● Strict quality control system: strict QC standard of sequencing data.
Sequencing platform & Sequencing strategy |
Data amount |
Concentration
|
Volume |
Amount |
QC result |
Novaseq system, paired-end 150bp |
X≤30Gb |
≥1ng/μl |
≥20 μl |
≥20ng |
Qualified |
30Gb < X≤100Gb |
≥1ng/μl |
≥25 μl |
≥30ng |
||
X>100Gb |
≥2ng/μl |
≥25 μl |
≥50ng |
In addition to concentration and total amount, a suitable peak pattern is also required.
Platform
|
Concentration
|
Volume |
QC result |
HiseqXten |
≥2.5nM |
≥20μL |
Qualified |
NovaSeq 6000 S4 |
≥2nM |
≥30μL |
|
NovaSeg 6000 SP |
≥2nM |
≥30μL |
Data Quality Assessment of RNA Sample
Table 1. Statistics on sequencing data.
Sample ID |
BMKID |
Raw reads |
Raw Data (bp) |
Clean reads (%) |
Q20(%) |
Q30(%) |
GC(%) |
C_01 |
BMK_01 |
22,870,120 |
6,861,036,000 |
96.48 |
99.14 |
94.85 |
36.67 |
C_02 |
BMK_02 |
14,717,867 |
4,415,360,100 |
96.00 |
98.95 |
93.89 |
37.08 |
Figure 1. Quality distribution along reads in each sample
Figure 2. Base content distribution
Figure 3. Distribution of read contents in sequencing data