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DNA/RNA Sequencing – Illumina Sequencer

Illumina sequencing technology, sequencing by synthesis (SBS), is a widely adopted next-generation sequencing (NGS) technology worldwide, responsible for generating more than 90% of the world’s sequencing data. A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all 4 reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. The method virtually eliminates errors and missed calls associated with strings of repeated nucleotides (homopolymers). Illumina sequencing by synthesis technology supports both single-read and paired-end libraries. SBS technology offers a short-insert paired-end capability for high-resolution genome sequencing, as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, and more. The combination of short inserts and longer reads increases the ability to fully characterize any genome.


Service Details

Demo Result

Service Details Features

Platform

Sequencing Mode

Applications

Illumina Nova system

SE50

PE50

PE150

PE250

1. Rapidly sequence whole genomes

2. Zoom in to deeply sequence target regions

3. Utilize RNA sequencing (RNA-Seq) to discover novel RNA variants and splice sites, or quantify mRNAs for gene expression analysis

4. Analyze epigenetic factors such as genome-wide DNA methylation and DNA-protein interactions

5. Sequence cancer samples to study rare somatic variants, tumor subclones, and more Study microbial diversity in humans or in the environment

Service Advantages

● Extensive experience on Illumina sequencing platform with thousands of closed projects with various species.
● Mature sequencing process with short turn-around time.
● Complete downstream bioinformatics analysis services.
● Strict quality control system: strict QC standard of sequencing data.

Sample Requirements*

Sequencing platform &

Sequencing strategy

Data amount

 

Concentration
(Thermo Life Qubit 3.0)

 

Volume

Amount 

QC result

Novaseq system,

paired-end 150bp

X≤30Gb

≥1ng/μl  

 ≥20 μl  

≥20ng

Qualified 

30Gb < X≤100Gb

≥1ng/μl

 ≥25 μl

≥30ng

X>100Gb

≥2ng/μl

≥25 μl 

≥50ng

Note:
  • In addition to concentration and total amount, a suitable peak pattern is also required.

Single Lane (Illumina)

Platform 

 

 

Concentration
(qPCR nM)

 

Volume

QC result

 HiseqXten

≥2.5nM

≥20μL

Qualified 

NovaSeq 6000 S4

 ≥2nM

≥30μL

NovaSeg 6000 SP

≥2nM 

≥30μL

 

Please reach out to us if your samples don't meet the starting material requirements.

Service Workflow

sample preparation

Library quality control

Sequencing

Sequencing

Data analysis

Data quality control

Sample QC

Project delivery


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  • Data Quality Assessment of RNA Sample

    Table 1. Statistics on sequencing data.

    Sample ID

    BMKID

    Raw reads

    Raw Data (bp)

    Clean reads (%)

    Q20(%)

    Q30(%)

    GC(%)

    C_01

    BMK_01

    22,870,120

    6,861,036,000

    96.48

    99.14

    94.85

    36.67

    C_02

    BMK_02

    14,717,867

    4,415,360,100

    96.00

    98.95

    93.89

    37.08

    Figure 1. Quality distribution along reads in each sample

    A9

    Figure 2. Base content distribution

    A10

    Figure 3. Distribution of read contents in sequencing data

    A11

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