● Platforms: Illumina NovaSeq 6000 and NovaSeq X Plus
● Sequencing modes: PE150 and PE250
● Quality control of libraries before sequencing
● Sequencing data QC and delivery: delivery of QC report and raw data in fastq format after demultiplexing and filtering Q30 reads
● Versatility of Sequencing services: the customer may choose to sequence by lane, flow cell, or by amount of data required (partial lane sequencing).
● Extensive experience on Illumina sequencing platform: with thousands of closed projects with various species.
● Delivery of sequencing QC report: with quality metrics, data accuracy and overall performance of the sequencing project.
● Mature sequencing process: with short turn-around time.
● Rigorous Quality Control: we implement strict QC requirements to guarantee the delivery of consistently high-quality results.
Platform |
Flow Cell |
Sequencing mode |
Unit |
Estimated output |
NovaSeq X |
10B (8 lanes) |
PE150 |
Single Lane Partial Lane |
375Gb / Lane |
25B ( 8 lanes) |
PE150 |
Single Lane Partial Lane |
1000 Gb/Lane |
|
NovaSeq 6000 |
SP Flow cell (2 lanes) |
PE250 |
Flow Cell Single Lane Partial Lane |
325-400 M reads / Lane |
S4 Flow cell (4 lanes) |
PE150 |
Flow Cell Single Lane Partial Lane |
~800 Gb / Lane |
Data Amount (X) |
Concentration (qPCR/nM) |
Volume |
|
Partial Lane Sequencing
|
X ≤ 10 Gb |
≥ 1 nM |
≥ 25 μl |
10 Gb < X ≤ 50 Gb |
≥ 2 nM |
≥ 25 μl |
|
50 Gb < X ≤ 100 Gb |
≥ 3 nM |
≥ 25 μl |
|
X > 100 Gb |
≥ 4 nM |
≥ 25 μl |
|
Lane Sequencing |
Per Lane |
≥ 1.5 nM / Library pool |
≥ 25 μl / Library pool |
In addition to concentration and total amount, a suitable peak pattern is also required.
Note: Lane sequencing of low diversity libraries requires PhiX spike-in to ensure robust base calling.
We recommend submitting pre-pooled libraries as samples. If you require BMKGENE to perform library pooling, please refer to
the library requirements for partial lane sequencing.
Main peak should be within 300-450 bp.
Libraries should have a single main peak, no adapter contamination and no primer dimers.
A report on the quality of the library is provided before sequencing, assessing library amount, and fragmentation.
Table 1. Statistics on sequencing data.
Sample ID |
BMKID |
Raw reads |
Raw Data (bp) |
Clean reads (%) |
Q20(%) |
Q30(%) |
GC(%) |
C_01 |
BMK_01 |
22,870,120 |
6,861,036,000 |
96.48 |
99.14 |
94.85 |
36.67 |
C_02 |
BMK_02 |
14,717,867 |
4,415,360,100 |
96.00 |
98.95 |
93.89 |
37.08 |
Figure 1. Quality distribution along reads in each sample
Figure 2. Base content distribution
Figure 3. Distribution of read contents in sequencing data