Products

DNBSEQ pre-made libraries

DNBSEQ, developed by MGI, is an innovative NGS technology that has managed to decrease further down the sequencing costs and increase throughput. Preparation of DNBSEQ libraries involves DNA fragmentation, preparation of ssDNA, and rolling circle amplification to obtain the DNA nanoballs (DNB). These are then loaded onto a solid surface and subsequently sequenced by combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ technology combines the advantages of having a low amplification error rate with using high density error patterns with nanoballs, resulting in sequencing with higher throughput and accuracy.

Our pre-made library sequencing service enables customers to prepare Illumina sequencing libraries from diverse sources (mRNA, whole genome, amplicon, 10x libraries, among others), which are converted to MGI libraries in our laboratories to be sequenced in DNBSEQ-T7, enabling high data amounts at lower costs.

Service Details

Demo Result

Features

● Platform: MGI-DNB-T7

● Sequencing modes: PE150

● Transfer of Illumina libraries to MGI: enabling sequencing of high data volumes at low cost.

● Quality control of libraries before sequencing.

● Sequencing data delivery and QC: delivery of QC report and raw data in fastq format after demultiplexing and filtering Q30 reads.

Advantages

● Versatility of Sequencing services: the customer may choose to sequence by lane or amount of data.

● High data output: 1400 Gb/lane

● Delivery of sequencing QC report: with quality metrics, data accuracy and overall performance of the sequencing project.

● Mature sequencing process: with short turn-around time.

● Rigorous Quality Control: we implement strict QC requirements to guarantee the delivery of consistently high-quality results.

Sample Requirements

	Data Amount (X)	Concentration (qPCR/nM)	Volume
Partial Lane	X ≤ 50 Gb	≥ 2 nM	≥ 20 μl
	50 Gb ≤ X < 100 Gb	≥ 3 nM	≥ 20 μl
	X ≥ 100 Gb	≥ 4 nM	≥ 20 μl
Single Lane		≥ 1.5 nM	≥ 25 μl

In addition to concentration and total amount, a suitable peak pattern is also required.

Service Workflow

Previous: Hi-C based Chromatin Interaction

Next:

Library QC report

A report on the quality of the library is provided before sequencing, assessing library amount, and fragmentation.

Sequencing QC report

Table 1. Statistics on sequencing data.

Sample ID	BMKID	Raw reads	Raw Data (bp)	Clean reads (%)	Q20(%)	Q30(%)	GC(%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08