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High Performance Genome Annotation - Full-length mRNA sequencing-Nanopore – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Alternative splicing
ü Expression quantification in gene level and isoform level
ü Differential expression analysis
ü Function annotation and enrichment (DEGs and DETs)

Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Purity |
Integrity |
Amount |
| OD260/280≥1.8;OD260/230≥1.0;Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel. |
For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation |
Conc. ≥40 ng/μl;Total ≥ 1 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow

Sample delivery

Library construction

Sequencing

Data analysis

After-sale services
Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services
Product detail pictures:
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We normally think and practice corresponding on the change of circumstance, and grow up. We aim at the achievement of a richer mind and body and also the living for High Performance Genome Annotation - Full-length mRNA sequencing-Nanopore – Biomarker , The product will supply to all over the world, such as: Thailand, London, Turkmenistan, We aim to become the modern enterprise with the commercial ideal of "Sincerity and confidence" and with the aim of "Offering customers the most sincere services and best quality products". We sincerely ask for your unchanged support and appreciate your kind advice and guidance.