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Biomarker Technologies Pom Zoo 31 Ua tiavDe novKev Tshawb Fawb Genome hauv 2021

Xyoo 2021, BMKGENE tau pom 31 De novo genome kev tshawb fawb tau ua tiav hauv cov ntawv xov xwm cuam tshuam nrog tag nrho cov cuam tshuam ntau dua 320. 15 ntawm cov ntawv tau sau 4 ntawm lawv tau sau ua thawj tus sau los ntawm BMKGENE

Txoj cai tom qab pib xyoo 2022, twb tau muaj ob tsab ntawv tshawb fawb luam tawm hauv phau ntawv journal "Natural Genetics" thiab "Molecular Plant" feem.Lawv yog, "Ob qhov sib txawv haplotypes los ntawm cov lychee genome uas muaj ntau heterozygous lychee genome qhia kev ywj pheej domestication txheej xwm rau thaum ntxov thiab lig-maturing cultivars" (Lychee genome research, ua los ntawm College of Horticulture, South China Agricultural university, thiab scientific collaborators, luam tawm nyob rau hauv Natural Genetics. ), thiab "Genome sequences ntawm tsib Sitopsis hom Aegilops thiab keeb kwm ntawm polyploid hom qoob mog B-subgenome" (Tsib Sitopsis hom genome, ua los ntawm xib fwb Bao Liu cov kev tshawb fawb pab neeg ntawm Northeast Normal University.).Peb kuj yuav tshuaj xyuas ob kab lus no thiab qhia rau peb cov neeg nyeem.

Tam sim no, cia peb saib ntawm cov ntawv tshawb fawb zoo tshaj plaws luam tawm ntawm 2021 co-sau los ntawm BMK thiab peb cov chaw sib koom tes.

Cog Genome - Kev tawg ntawm ntau hom.

1.A qhov zoo tshaj plaws genome los ua ke qhia txog rye genomic yam ntxwv thiab agronomically tseem ceeb noob

Kev Sib Koom Tes: Henan Agricultural University

Phau ntawv Journal: Natural Genetics

Impact Factor: 38.31

Hauv qhov project no, genome ntawm Weining rye, cov neeg tseem ceeb suav rye ntau yam, tau ua raws.Cov contigs sib sau ua ke (7.74 Gb) suav txog 98.47% ntawm qhov kwv yees genome loj (7.86 Gb), nrog 93.67% ntawm contigs (7.25 Gb) muab rau xya chromosomes.Cov ntsiab lus rov ua dua yog 90.31% ntawm cov genome sib sau ua ke.Kev soj ntsuam ntxiv ntawm Weining los ua ke tso lub teeb tshiab ntawm genome-wide gene duplications thiab lawv qhov cuam tshuam rau cov hmoov txhuv nplej siab biosynthesis noob, lub cev lub koom haum ntawm complex prolamin loci, gene qhia cov ntsiab lus nyob rau hauv thaum ntxov heading zoo thiab putative domestication-koom nrog chromosomal cheeb tsam thiab loci nyob rau hauv rye.Qhov kev sib tw genome no tau cog lus tias yuav ua kom cov kev tshawb fawb genomic thiab kev yug me nyuam hauv rye thiab cov qoob loo muaj feem xyuam.

2.Rose tsis muaj prickle: genomic insights txuas rau ya raws adaptation

Kev Sib Koom Tes: Kunming Institute of botany, Suav Academy ntawm Kev Tshawb Fawb

Phau ntawv Journal: National Science Review

Qhov cuam tshuam: 17.273

Hauv qhov project no, cov qauv ntawm 'Basye's Thornless' (BT, prickle-free cultivar of Rosa wichuraiana), 'Old Blush' (OB, tus tsim genotype hauv Rose domestication), lawv F1 hybrids thiab BCF1 tau sau.Thiab qhov zoo tshaj plaws siv genome los ua ke tau tsim los txheeb xyuas cov caj ces cuam tshuam nrog kev txhim kho qia prickle.Lub genome loj yog hais txog 530.6 Mb.Txhawm rau txheeb xyuas qhov zoo ntawm cov genome sib sau ua ke, kev tshuaj xyuas xws li genetic map piv, BUSCO, NGS nyeem reassembly, sib piv nrog OB haplotype, sequencing base error rate control and genome-wide LTR Assembly Index value check (LAI = 20.03) tau ua.Qhov kev tshawb fawb no nthuav tawm cov qauv qub txeeg qub teg thiab kev tswj hwm txoj cai ntawm qia prickles thiab muab peb lub hauv paus thiab cov peev txheej tshiab los kawm txog Rose biology thiab kuv cov cim molecular cuam tshuam nrog cov yam ntxwv tseem ceeb.

3.Whole-Genome Sequence ntawm Synthesized Allopolyploids hauv Cucumis Qhia Kev Pom Zoo rau Genome Evolution ntawm Allopolyploidization

Kev Sib Koom Tes: Nanjing Agricultural University

Phau ntawv Journal: Advanced Science

Qhov cuam tshuam: 16.801

Txoj kev tshawb no tau tshaj tawm cov genome zoo ntawm cov khoom hluavtaws allotetraploid tau siv kev sib xyaw ua ke ntawm cov dib (C. sativus, 2n = 14) thiab nws cov txheeb ze tsiaj qus (C. hystrix, 2n = 24) thiab tom qab chromosome duplication, uas yog thawj zaug. tag nrho sequenced hluavtaws allopolyploid.Lub rooj sib txoos ntawm cov genome tau siv qhov kev ua haujlwm ntawm "PacBio + BioNano + Hi-C + Illumina" ua ke, ua rau genome loj ntawm 530.8Mb thiab contig N50 = 6.5Mb.Kev nyeem tau raug xa mus rau 19 pseudochromosomes thiab subgenomes.Cov txiaj ntsig tau qhia tias kev sib xyaw ua ke, tsis yog kev sib tw genome, ua rau feem ntau ntawm cov kev hloov genomic hauv nuclear thiab cp genomes.Nws tau pom tias qhov ruaj khov heterozygosity muab C. × hytivus nrog kev hloov pauv kev ntxhov siab ntxiv.Cov txiaj ntsig tau muab kev nkag siab tshiab rau hauv cov nroj tsuag polyploidy evolution thiab muab lub tswv yim zoo rau kev cog qoob loo rau yav tom ntej.

4.Comparative Genome Analyzes Highlight Transposon Mediated Genome Expansion thiab Evolutionary Architecture ntawm 3D Genomic Folding hauv paj rwb

Kev Sib Koom Tes: Huazhong Agricultural University

Phau ntawv Journal: Molecular Biology thiab Evolution

Qhov cuam tshuam: 16.242

Qhov project no siv Nanopore Sequencing los sib sau cov genome ntawm peb hom paj rwb uas yog: Gossypium rotundifolium (K2, genome loj = 2.44Gb, contigN50 = 5.33Mb), G. arboreum (A2, genome loj = 1.62Gb, contig1.50), contigN50 G. raimondii (D5, genome size = 0.75Gb, contigN50 = 17.04 Gb).Tshaj 99% ntawm tag nrho peb genomes tau sib sau ua ke los ntawm Hi-C.Cov txiaj ntsig ntawm BUSCO tsom xam yog 92.5%, 93.9% thiab 95.4% feem.Tag nrho cov lej no tau qhia tias peb lub genomes sib dhos yog siv-qib.Kev sib piv genome soj ntsuam tau sau cov ntsiab lus ntawm cov kab mob tshwj xeeb TE amplification ua rau muaj qhov sib txawv loj ntawm genome.Txoj kev tshawb no pom lub luag haujlwm ntawm transposon-mediated genome expansion nyob rau hauv evolution ntawm ntau dua-order chromatin qauv hauv cov nroj tsuag.

5.Chromosome-scale sib dhos thiab tsom xam ntawm biomass qoob loo Miscanthus lutarioriparius genome

Kev Sib Koom Tes: CAS Center for Excellence in molecular Plant Sciences

Phau ntawv Journal: Nature Communications

Qhov cuam tshuam: 14.912

Qhov project no tau tshaj tawm cov chromosome-scale sib dhos ntawm Miscanthus lutarioriparius genome los ntawm kev sib txuas ntawm Oxford Nanopore sequencing thiab Hi-C technologies.Kev sib dhos 2.07Gb npog 96.64% ntawm genome, nrog contig N50 ntawm 1.71 Mb.Kwv yees li 94.30% ntawm tag nrho cov kab ke tau muab tso rau hauv 19 pseudochromosomes.Los ntawm kev sib piv nrog BAC ib ntus, LAI kev ntsuam xyuas, kev ntsuam xyuas BUSCO, rov sib sau ua ke nrog NGS cov ntaub ntawv, rov sib sau ua ke ntawm cov ntaub ntawv transcriptome, cov genome tau soj ntsuam zoo thiab txuas ntxiv.Allotetraploid keeb kwm ntawm M. lutarioriparius tau lees paub tias siv centromeric satellite rov ua dua.Tandem duplicate noob ntawm M. lutarioriparius yog functional enriched tsis tau tsuas yog nyob rau hauv cov nqe lus hais txog kev nyuaj siab teb, tab sis cell phab ntsa biosynthesis.Gene duplicates tej zaum ua lub luag haujlwm hauv C4 photosynthesis thiab ua rau Miscanthus C4 photosynthesis ntawm qhov kub thiab txias.Cov kev tshawb fawb tau muab cov ntaub ntawv tseem ceeb rau kev kawm cov nroj tsuag perennial.

6.Ib chromosome-theem Camptotheca acuminata genome los ua ke muab kev nkag siab rau hauv evolutionary keeb kwm ntawm camptothecin biosynthesis

Kev Sib Koom Tes: Sichuan University

Phau ntawv Journal: Nature Communications

Qhov cuam tshuam: 14.912

Qhov project no tau tshaj tawm qhov zoo, chromosome-theem C. acuminata genome los ua ke, nrog genome loj ntawm 414.95Mb thiab contingN50 1.47Mb.Peb pom tias C. acuminata muaj kev ywj pheej tag nrho-genome duplication thiab ntau cov noob tau los ntawm nws muaj feem xyuam rau camptothecin biosynthesis.Kev ua haujlwm sib txawv ntawm LAMT noob thiab kev hloov pauv zoo ntawm ob lub noob SLAS, yog li ntawd, ob qho tib si tau pab txhawb rau camptothecin biosynthesis hauv C. acuminata.Cov txiaj ntsig tau hais txog qhov tseem ceeb ntawm kev sib koom ua ke genome zoo hauv kev txheeb xyuas cov kev hloov pauv caj ces hauv cov keeb kwm evolutionary ntawm cov metabolite theem nrab.

7.Allele-txhais genome qhia txog kev sib txawv ntawm biallelic thaum lub sij hawm cassava evolution

Kev Sib Koom Tes: Suav Academy ntawm Tropical Agricultural Sciences

Phau ntawv: Molecular Plant

Qhov cuam tshuam: 13.162

Qhov project no tau sib sau ua ke ib qho kev siv genome rau cassava nrog contigN50 1.1Mb siv Pacific Biosciences (PacBio) sequencing platform.Tom qab kev ntsuam xyuas los ntawm BUSCO, LAI Performance index, thiab high-density genetic map, cov genome sib sau ua ke tau lees paub tias yog qib qib refence.Cov cheeb tsam redundant tau txheeb xyuas thiab contigs tau thauj mus rau 18 pseudochromosomes siv Hi-C txuas.Qhov zoo tshaj plaws thiab allele-txhais siv cov genome rau cov qos yaj ywm yog qhov tseem ceeb hauv kev txheeb xyuas qhov sib txawv ntawm ob-alleles ntawm homologous chromosomes, tso cai rau tshawb nrhiav qhov sib txawv thiab qhia txog kev tswj hwm ntawm bi-alleles thiab lawv cov kev hloov pauv hloov pauv ntawm kev tsav tsheb.Nws pab txhawb kev tsim cov tswv yim tshiab hauv cov qos yaj ywm thiab lwm cov qoob loo heterozygous.

8.Genomic insights rau kev loj hlob sai ntawm paulownias thiab tsim ntawm Paulownia witches 'broom

Kev Sib Koom Tes: Henan Agricultural University

Phau ntawv: Molecular Plant

Qhov cuam tshuam: 13.162

Qhov project no tau sib sau ua ke cov khoom siv hluav taws xob zoo ntawm Paulownia fortunei, 511.6 Mb hauv qhov loj me, nrog 93.2% ntawm cov kab ke ua ke rau 20 pseudochromosomes.Ntau dua photosynthetic efficiency yog ua tiav los ntawm kev sib koom ua ke C3 photosynthesis thiab crassulacean acid metabolism txoj hauv kev, uas tej zaum yuav tau pab txhawb kev loj hlob sai heev ntawm paulownia ntoo.Ntxiv genome sequencing ntawm PaWB phytoplasma, ua ke nrog functional analyses, qhia tias tus effector PaWB-SAP54 ncaj qha cuam tshuam nrog Paulownia PfSPLa, uas nyob rau hauv lem ua rau degradation ntawm PfSPLa los ntawm ubiquitin-mediated txoj kev thiab ua rau tsim ntawm witches 'broom.Cov ntaub ntawv muab kev nkag siab tseem ceeb rau hauv biology ntawm paulownias thiab kev tswj hwm txoj cai rau kev tsim PaWB.

Tsiaj genome - Kev nkag siab tob ntawm hom evolution

1.Lub genome ntawm Nautilus pompilius illuminates qhov muag evolution thiab biomineralization

Kev Sib Koom Tes: South China Sea Institute of Oceanology, CAS

Phau ntawv Journal: Natural Ecology & evolution

Qhov cuam tshuam: 15.462

Qhov project no nthuav tawm cov genome tiav rau Nautilus pompilius.Nws muaj qhov tsawg kawg nkaus genome ntawm cov kab ke cephalopods, uas yog 730.58Mb nrog contigN50 = 1.1Mb.Qhov kev ntsuam xyuas BUSCO yog 91.31%.Ua ke nrog transcriptome, proteome, gene tsev neeg thiab phylogenetic tsom xam, qhov genome no tau muab cov ntsiab lus siv ntawm cephalopod nrhav, xws li lub qhov muag pinhole thiab biomineralization.Kev tshawb fawb tau qhia tias kev puas tsuaj ntawm qhov ua tiav ntawm Hox gene pawg yuav cuam tshuam nrog lub plhaub ploj ntawm Mollusks.Qhov tseem ceeb, ntau yam kev hloov pauv genomic suav nrog kev poob noob, kev cog lus ywj pheej thiab nthuav dav ntawm cov tsev neeg tshwj xeeb gene thiab lawv cov kev tswj hwm kev sib raug zoo yuav ua rau cov evolution ntawm nautilus pinhole qhov muag.Lub nautilus genome tsim muaj txiaj ntsig zoo rau kev tsim kho cov xwm txheej hloov pauv hloov pauv thiab kev tsim kho genomic uas tsim cov cephalopods uas tseem tshuav.

2.Seadragon genome tsom xam muab kev nkag siab rau nws cov phenotype thiab kev txiav txim siab poj niam txiv neej

Kev Sib Koom Tes: South China Sea Institute of Oceanology, CAS

Phau ntawv Journal: Science Advances

Qhov cuam tshuam: 14.132

Qhov project de novo-sequenced txiv neej thiab poj niam genomes ntawm cov seadragon (Phyllopteryx taeniolatus) thiab nws cov tsiaj muaj feem xyuam nrog, cov kab nuv ntses pipefish (Syngnathoides biaculeatus).Lub genome loj rau Phyllopteryx taeniolatus yog ~ 659 Mb (♂) thiab ~ 663 Mb (‍♀), nrog contigN50 ntawm 10.0Mb thiab 12.1mb.lub genome loj rau Phyllopteryx taeniolatus yog 637 Mb (♂) thiab ~ 648 Mb (‍♀), nrog contigN50 ntawm 18.0Mb thiab 21.0Mb.Los ntawm kev txheeb xyuas phylogenetic, cov seadragon thiab cov ntses ntses ntses yog cov viv ncaus ntawm Syngnathinae, thiab sib txawv ntawm 27.3 Ma dhau los.Kev hloov pauv cov ntaub ntawv los ntawm kev hloov pauv hloov tshiab, cov nplooj zoo li appendages, qhia tau hais tias ib txheej ntawm cov noob feem ntau koom nrog hauv kev loj hlob fin tau koom ua ke nrog rau kev txhawb nqa ntawm tran-scripts rau cov ntaub so ntswg kho thiab tiv thaiv kab mob.Ib qho kev sib deev-kev txiav txim siab qhov chaw encoding tus txiv neej tshwj xeeb amhr2y noob sib koom los ntawm cov seadragon thiab alligator pipefish tau txheeb xyuas.Qhov project no tau muab pov thawj tseem ceeb rau kev kawm txog kev hloov pauv hloov pauv.


Post lub sij hawm: Sep-19-2022

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