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Usoro metagenomic -NGS
Metagenome na-ezo aka mkpokọta ihe mkpụrụ ndụ ihe nketa nke obodo agwakọta dị ka metagenome gburugburu ebe obibi, metagenome mmadụ, wdg.Usoro metagenomic bụ ngwá ọrụ molekụla eji enyocha ihe agwakọta genomic nke ewepụtara site na nlele gburugburu ebe obibi, nke na-enye ozi zuru ezu na ụdị dị iche iche na ụbara, nhazi ọnụ ọgụgụ mmadụ, mmekọrịta phylogenetic, mkpụrụ ndụ ihe nketa na-arụ ọrụ na netwọk mmekọrịta yana ihe gburugburu ebe obibi.
Platform:Illumina NovaSeq Platform
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Usoro Metagenomic-Nanopore
Metagenomics bụ ngwá ọrụ molekụla eji nyochaa ihe ngwakọta genomic nke ewepụtara site na ihe atụ gburugburu ebe obibi, nke na-enye ozi zuru ezu na ụdị dị iche iche na ụbara, nhazi ọnụ ọgụgụ mmadụ, mmekọrịta phylogenetic, mkpụrụ ndụ ihe nketa na-arụ ọrụ na netwọk mmekọrịta na ihe gburugburu ebe obibi, wdg. Nanopore usoro nyiwe ka ewebatala nso nso a. na ọmụmụ metagenomic.Ọrụ ya pụtara ìhè n'ogologo ọgụgụ na-akwalite nyocha nke metagenomic iyi, ọkachasị mgbakọ metagenome.N'iji uru nke ogologo ọgụgụ ọgụgụ, ọmụmụ metagenomic dabere na Nanopore na-enwe ike nweta mgbakọ na-aga n'ihu na-atụnyere metagenomics egbe.E bipụtala ya na metagenomics dabere na Nanopore rụpụtara nke ọma na mkpụrụ ndụ nje mechiri emechi site na microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Platform:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Nke a dị na 16S na 18S rRNA nwere ma mpaghara echekwara nke ukwuu yana hyper-variable bụ akara mkpisiaka mkpụrụ ndụ zuru oke maka njirimara prokaryotic na eukaryotic.Na-ewere uru nke sequencing, ndị a amplicons nwere ike ezubere iche dabere na ndị echekwara akụkụ na hyper-variable mpaghara nwere ike n'ụzọ zuru ezu ji maka microbial njirimara na-enye aka ọmụmụ ekpuchi microbial diversity analysis, taxonomy, phylogeny, wdg Single-molecule ozugbo (SMRT) ) usoro nke PacBio ikpo okwu na-enyere aka inweta nke ukwuu ziri ezi ogologo agụ, nke nwere ike na-ekpuchi zuru-ogologo amplicon (ihe dị ka 1.5 Kb).Echiche sara mbara nke mkpụrụ ndụ ihe nketa mere ka mkpebi siri ike dị na nkọwa ụdịdị na nje bacteria ma ọ bụ fungi.
Platform:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing bu n'obi ikpughe phylogeny, taxonomy, na ụdị dị ukwuu n'ime obodo microbial site na nyocha ngwaahịa PCR nke ihe nrịbama mkpụrụ ndụ ihe nketa nke nwere ma akụkụ a kparịtara ụka na nke ukwuu.Iwebata akara mkpisi aka molikula zuru oke site na Woeses et al, (1977) na-enye ike ịkọwapụta microbiome na-enweghị ikewapụ.Usoro nke 16S (bacteria), 18S (fungi) na Internal transcripted spacer(ITS, fungi) na-enye ohere ka a mata ụdị ma ụdị dị ukwuu yana ụdị dị ụkọ na nke amabeghị.Teknụzụ a aghọwo ihe a na-etinye n'ọtụtụ ebe na ngwá ọrụ bụ isi n'ịchọpụta ihe mejupụtara microbial dị iche iche na gburugburu ebe dị iche iche, dị ka ọnụ mmadụ, eriri afọ, nsị, wdg.
Platform:Illumina NovaSeq Platform
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Usoro njegharị nje na fungal dum Genome
Bacterial na fungal dum genome re-sequencing bụ ngwá ọrụ dị oke mkpa iji mezue genomes nke nje bacteria na fungi a ma ama, yana iji tụnyere ọtụtụ genome ma ọ bụ iji map genomes nke ihe ọhụrụ dị ndụ.Ọ dị oke mkpa ịhazi usoro genome niile nke nje bacteria na fungi iji mepụta mkpụrụ ndụ ihe nketa ziri ezi, mee nchọpụta microbial na ọmụmụ ihe ọmụmụ genome ndị ọzọ.
Platform: Illumina NovaSeq Platform
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Usoro Metatranscriptome
Metatranscriptome sequencing na-achọpụta mkpụrụ ndụ ihe nketa nke ụmụ nje (ma eukaryotes na prokaryotes) n'ime gburugburu ebe obibi (ya bụ ala, mmiri, oke osimiri, nsị, na eriri afọ) .Kpọmkwem, ọrụ a na-enye gị ohere ịnweta nkọwapụta mkpụrụ ndụ dum nke obodo microbial dị mgbagwoju anya, nyocha taxonomic. ụdị dị iche iche, nyocha nkwalite ọrụ nke mkpụrụ ndụ ihe nketa dị iche iche, na ndị ọzọ.
Platform: Illumina NovaSeq Platform
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Fungal Genome
Teknụzụ Biomarker na-enye nyocha genome, ezigbo genome na genome zuru oke nke fungal dabere na ebumnuche nyocha akọwapụtara.Enwere ike nweta usoro genome, mgbakọ na nkọwa ọrụ site na ijikọta usoro nke ọgbọ na-abịa + usoro ọgbọ nke atọ iji nweta mgbakọ genome dị elu.Enwere ike iji teknụzụ Hi-C rụọ ọrụ iji kwado mgbakọ genome na ọkwa chromosome.
Platform:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Nje bacteria zuru ezu Genome
Teknụzụ Biomarker na-enye ọrụ nhazi usoro maka iwulite genome nke nje bacteria na enweghị oghere efu.Isi ọrụ nje bacteria na-arụ ọrụ genome zuru oke gụnyere usoro ọgbọ nke atọ, mgbakọ, nkọwa ọrụ na nyocha bioinformatic dị elu na-emezu ebumnuche nyocha.Nkọwapụta nke genome nke nje bacteria na-enye ike ikpughe usoro ndị dị mkpa na-adabere na usoro ndu ha, nke nwekwara ike inye nkọwa bara uru maka nchọpụta mkpụrụ ndụ ihe nketa na ụdị eukaryotic dị elu.
Platform:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
