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Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)

Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq) egosipụtara
  • Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
  • Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
  • Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
  • Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
  • Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)

ATAC-seq bụ usoro usoro ntinye dị elu maka nyocha nke nnweta chromatin genome, nke dị mkpa maka njikwa epigenetic zuru ụwa ọnụ nke mkpụrụ ndụ ihe nketa.A na-etinye ihe nkwụnye usoro n'ime mpaghara chromatin mepere emepe site na transposase Tn5 hyperactive.Mgbe PCR nwechara, a na-arụ ọba akwụkwọ nke usoro.Enwere ike nweta mpaghara chromatin niile mepere emepe n'okpuru ọnọdụ oge-oge, ọ bụghị naanị na saịtị njikọ nke ihe odide ederede, ma ọ bụ mpaghara mpaghara gbanwetụrụ.


Nkọwa ọrụ

Bioinformatics

Nsonaazụ ngosi

Uru ọrụ

● Nyocha zuru oke: pipeline nyocha ọkachamara iji gboo mkpa nyocha dị iche iche ahaziri;

● Ahụmahụ sara mbara: Ọtụtụ narị ọrụ arụchara nke ọma ruo ugbu a, gụnyere anụmanụ na ihe ọkụkụ.

Nkọwapụta ọrụ

Platform: Illumina NovaSeq ikpo okwu

Ụdị ọba akwụkwọ: ATAC-seq

Mbupute data akwadoro: ≥20M na-agụ

Ihe atụ chọrọ

Ụdị nlele: anụ ahụ, mkpụrụ ndụ dị ndụ, ma ọ bụ mkpụrụ ndụ oyi kpọnwụrụ na ihe ndị ọzọ

Nọmba ekwentị: ≥ 5×105mkpụrụ ndụ, nọmba cell ziri ezi bụ isi ihe na-eme ka ihe ịga nke ọma nke nnwale;

Arọ anụ ahụ: ≥ 200mg anụ ahụ ọhụrụ;

Ọbara: ≥2 ml

Usoro ọrụ ọrụ

Ihe atụ QC

Nnwale imewe

nnyefe sample

Nbufe ihe atụ

Nnwale pilot

Mwepụta DNA

Nkwadebe ụlọ akwụkwọ

Ịrụ ụlọ akwụkwọ

Usoro

Usoro

Nyocha data

Nyocha data

Mgbe ire Ọrụ

Mgbe-ire ọrụ

  • Nke gara aga:
  • Osote:

    • 表观-02

    1.Heatmap na ATAC na-agụ nkesa na TSS na mpaghara ndị dị n'akụkụ (± 3 kb)

    3 (1)2. Nkesa mpaghara chromatin mepere emepe na akụkụ genome dị iche iche

    4 (1)

    3.Differential elu oku n'etiti otu

    5 (1)

    nweta ntinye okwu

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