Well-designed Hi-C Sequencing - LncRNA – Biomarker
Well-designed Hi-C Sequencing - LncRNA – Biomarker Detail:
Bioinformatics
Raw data quality control
Alignment with reference genome
Transcript assembly
Correlation analysis
Known lncRNA identification
Coding potential of candidate lncRNA
Regulatory network: lncRNA-mRNA
lncRNA expression quantification
Differentially expressed lncRNA (DE-lncRNA)
PCA
WGCNA
LncRNA target gene prediction
GO and KEGG enrichment on DE-lncRNA target genes
Novel lncRNA
Alternative splicing prediction
mRNA expression analysis
Comparative analysis lncRNA vs. mRNA
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