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Whole genome bisulfite sequencing

DNA methylation at the fifth position in cytosine (5-mC) has a fundamental influence on gene expression and cellular activity. Abnormal methylation patterns have been associated with several conditions and diseases, such as cancer. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.

Platform: Illumina NovaSeq Platform


Service Details

Bioinformatics

Demo Results

Service Advantages

●  Complete platform: provide one-stop excellent service from sample processing, library construction, sequencing to bioinformatics analysis.

●  High accuracy: Mature methylation conversion processing technology can accurately analyze the methylation status of a single C base.

●  Wide coverage: detection of methylation sites at the genome-wide level.

●  Good repeatability: stable processing, suitable for comparative analysis among multiple samples.

 

Sample Specifications

 

Platform

 

 

Read Length

 

 

Sequencing Strategy

 

Illumina

PE 150

30X, three biological replicates, with high quality reference genome 

Sample Requirements

 

Sample Type

 

 

Amount (μg)

 

 

Conc. (ng/μl)

 

 

Volume (μl)

 

 

Purity

 

gDNA

≥ 2

≥ 20

 

≥ 20

 

Limited degradation and contamination

 

 

 

Service Work Flow

sample delivery

Sample delivery

Pilot experiment

DNA extraction

Library Preparation

Library construction

Sequencing

Sequencing

Data analysis

Data analysis

数据上传-01

Data delivery


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  •  Bioinformatics

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    1.Genome-wide DNA methylation distribution

    3(1)

    2.Annotation on highly-methylated CGI region

    4(1)

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