● Complete platform: provide one-stop excellent service from sample processing, library construction, sequencing to bioinformatics analysis.
● High accuracy: Mature methylation conversion processing technology can accurately analyze the methylation status of a single C base.
● Wide coverage: detection of methylation sites at the genome-wide level.
● Good repeatability: stable processing, suitable for comparative analysis among multiple samples.
DNA amount: ≥ 3 μg.
DNA concentration: ≥ 30 ng/μl.
Purity: OD260/280 = 1.8‒2.0 without degradation, protein or RNA contamination.
Platform: NovaSeq 6000 platform, PE150
Recommended Sequencing Depth :≥30× coverage
Bioinformatics
1.Genome-wide DNA methylation distribution
2.Annotation on highly-methylated CGI region