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Whole genome bisulfite sequencing

DNA methylation at the fifth position in cytosine (5-mC) has a fundamental influence on gene expression and cellular activity. Abnormal methylation patterns have been associated with several conditions and diseases, such as cancer. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.

Platform: Illumina NovaSeq6000


Service Details

Bioinformatics

Demo Results

Service Advantages

●  Complete platform: provide one-stop excellent service from sample processing, library construction, sequencing to bioinformatics analysis.

●  High accuracy: Mature methylation conversion processing technology can accurately analyze the methylation status of a single C base.

●  Wide coverage: detection of methylation sites at the genome-wide level.

●  Good repeatability: stable processing, suitable for comparative analysis among multiple samples.

 

Sample Requirements

DNA amount: ≥ 3 μg.

DNA concentration: ≥ 30 ng/μl.

Purity: OD260/280 = 1.8‒2.0 without degradation, protein or RNA contamination.

Service Specifications

Platform: NovaSeq 6000 platform, PE150

Recommended Sequencing Depth :≥30× coverage

Service Work Flow

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Experiment design

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Sample delivery

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DNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services


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  •  Bioinformatics

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    1.Genome-wide DNA methylation distribution

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    2.Annotation on highly-methylated CGI region

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