Whole genome bisulfite sequencing
Service Advantages
● Complete platform: provide one-stop excellent service from sample processing, library construction, sequencing to bioinformatics analysis.
● High accuracy: Mature methylation conversion processing technology can accurately analyze the methylation status of a single C base.
● Wide coverage: detection of methylation sites at the genome-wide level.
● Good repeatability: stable processing, suitable for comparative analysis among multiple samples.
Sample Specifications
|
Platform
|
Read Length
|
Sequencing Strategy
|
|
Illumina |
PE 150 |
30X, three biological replicates, with high quality reference genome |
Sample Requirements
|
Sample Type
|
Amount (μg)
|
Conc. (ng/μl)
|
Volume (μl)
|
Purity
|
|
gDNA |
≥ 2 |
≥ 20
|
≥ 20
|
Limited degradation and contamination
|
Service Work Flow
Sample delivery
DNA extraction
Library construction
Sequencing
Data analysis
Data delivery
Bioinformatics
1.Genome-wide DNA methylation distribution
2.Annotation on highly-methylated CGI region
