lncrna

Cov RNA ntev uas tsis yog coding (lncRNA) yog cov RNA ntev dua 200 nucleotides nrog cov peev xwm coding qis tab sis muaj cov haujlwm tswj hwm tseem ceeb. Lub BMKCloud lncRNA pipeline yog tsim los tshuaj xyuas cov tsev qiv ntawv rRNA depleted nrog cov genome zoo, zoo annotated, los ntawm kev tshuaj xyuas lncRNA thiab mRNA kev qhia ua ke. Tom qab nyeem trimming thiab kev tswj hwm zoo, nyeem tau sib phim rau cov genome siv los sib sau ua ke cov ntawv sau, thiab tom qab ntawd kev tshuaj xyuas cov qauv gene qhia txog lwm txoj kev splicing thiab cov noob tshiab. Cov ntawv sau tau txheeb xyuas tias yog mRNAs lossis lncRNAs, thiab kev tshuaj xyuas kev qhia sib txawv txheeb xyuas cov lncRNAs qhia sib txawv, lawv lub hom phiaj thiab cov noob qhia sib txawv (DEGS). Ob qho tib si DEGs thiab cov hom phiaj lncRNA qhia sib txawv tau sau tseg ua haujlwm kom nrhiav tau cov pawg ua haujlwm nplua nuj.