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ʻIkepili ʻAhahui Genome-wide
ʻO ka Genome-wide association study (GWAS) ka manaʻo e ʻike i nā ʻano ʻano genetic (genotype) e pili ana i nā ʻano kikoʻī (phenotype).Ke noiʻi nei ka haʻawina GWAS i nā hōʻailona genetic e hele i ka genome holoʻokoʻa o ka heluna nui o nā kānaka a wānana i nā hui genotype-phenotype ma o ka nānā ʻana helu helu ma ka pae heluna kanaka.Ua hoʻohana nui ʻia i ka noiʻi ʻana i nā maʻi kanaka a me ka hana ʻana i nā ʻano gene ma nā ʻano paʻakikī o nā holoholona a mea kanu paha.
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Hoʻokahi-nucleus RNA Sequencing
ʻO ka holomua o ka hopu ʻana i ke kelepona hoʻokahi a me ka ʻenehana kūkulu hale waihona puke me ka hui pū ʻana me ke kaʻina hana kiʻekiʻe e hiki ai i nā haʻawina hōʻike gene ma ke kumu cell-by-cell.Hāʻawi ia i kahi ʻōnaehana hohonu a piha hoʻi i nā heluna cell paʻakikī, kahi e pale nui ai i ka huna ʻana i ko lākou heterogeneity ma ka lawe ʻana i ka awelika o nā cell āpau.
Eia nō naʻe, ʻaʻole kūpono kekahi mau cell e hana ʻia i hoʻokuʻu ʻia hoʻokahi-cell, no laila, pono nā ʻano hana hoʻomākaukau ʻē aʻe - ka unuhi ʻana o ka nucleus mai nā ʻiʻo, ʻo ia hoʻi, lawe pololei ʻia ka nucleus mai nā ʻiʻo a i ʻole cell a hoʻomākaukau ʻia i hoʻokahi-nucleus suspension no hoʻokahi- ke kaʻina ʻana o ke kelepona.
Hāʻawi ʻo BMK i ka 10× Genomics ChromiumTM i hoʻokumu ʻia i ka lawelawe hoʻokaʻina RNA pū hoʻokahi.Ua hoʻohana nui ʻia kēia lawelawe i nā haʻawina e pili ana i nā haʻawina e pili ana i nā maʻi, e like me ka hoʻokaʻawale ʻana o ka cell immune, heterogeneity tumor, tissue development, etc.
ʻĀpana kope kope: 10× Genomics
Papahana: Illumina NovaSeq 6000
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Mea kanu/holoholona holoʻokoʻa Genome Sequencing
ʻO ka hoʻonohonoho hou ʻana o ka genome holoʻokoʻa, ʻike ʻia ʻo WGS, hiki ke hōʻike ʻia nā hoʻololi maʻamau a me nā ʻano like ʻole ma ka genome holoʻokoʻa me Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), a me Copy Number Variation (CNV). ).Hoʻokumu nā SV i kahi hapa nui o ka waihona hoʻololi ma mua o nā SNP a ʻoi aku ka nui o ka hopena i ka genome, he hopena koʻikoʻi i nā mea ola.ʻO ka heluhelu lōʻihi e hiki ai ke ʻike pololei i nā ʻāpana nui a me nā ʻano paʻakikī no ka mea ʻoi aku ka maʻalahi o ka heluhelu lōʻihi i ka hele ʻana o ka chromosomal ma nā wahi paʻakikī e like me ka tandem repeats, GC/AT-rich regions, a me hyper-variable regions.
Papahana: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
He kuleana koʻikoʻi ka hoʻonohonoho ʻana o nā cell i nā kaʻina hana ola like ʻole, e like me ka infiltration immune, embryo development, etc.Me ka hoʻomohala ʻana o ka ʻenehana, pono e aʻo ʻia ka morphology kikoʻī ultra-clear a me ka ʻokoʻa maoli o ka ʻōlelo molekika spatial me ka hoʻonā kiʻekiʻe.Hāʻawi ʻo BMKGENE i ka lawelawe hoʻokaʻina spatial transcriptome holoʻokoʻa, hoʻokahi manawa mai nā laʻana i nā ʻike olaola.
Ua hoʻoikaika nā ʻenehana transcriptomic spatial i nā hiʻohiʻona hou i nā ʻano noiʻi like ʻole ma o ka hoʻoholo ʻana i ka moʻolelo hōʻike gene me ka ʻike kikoʻī i nā ʻano like ʻole.
ʻO ka chip transcriptome āpau: BMKMANU S1000
Papahana: Illumina NovaSeq 6000
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10x Genomics Visium Spatial Transcriptome
ʻO ka Visium Spatial Gene Expression kahi ʻenehana transcriptome sequencing spatial no ka hoʻokaʻawale ʻana i nā kiko e pili ana i ka mRNA holoʻokoʻa.Palapala ʻāina i ka transcriptome holoʻokoʻa me ka pōʻaiapili morphological e ʻike i nā ʻike hou i ka hoʻomohala maʻamau, nā maʻi maʻi, a me ka noiʻi unuhi lapaʻau.Hāʻawi ʻo BMKGENE i ka lawelawe hoʻokaʻina spatial transcriptome holoʻokoʻa, hoʻokahi manawa mai nā laʻana i nā ʻike olaola.
Ua hoʻoikaika nā ʻenehana transcriptomic spatial i nā hiʻohiʻona hou i nā ʻano noiʻi like ʻole ma o ka hoʻoholo ʻana i ka moʻolelo hōʻike gene me ka ʻike kikoʻī i nā ʻano like ʻole..
Kipa transcriptome spatial: 10x Genomics Visium
Papahana:ʻO Illumina NovaSeq 6000
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ʻO ka lōʻihi mRNA Sequencing-Nanopore
He mea waiwai nui ka RNA sequencing no ka nānā ʻana i ka transcriptome piha.ʻO ka mea kānalua, ua hoʻokō ʻia nā hoʻomohala koʻikoʻi ma ʻaneʻi i ka heluhelu pōkole kuʻuna.Eia nō naʻe, loaʻa pinepine i nā palena i nā ʻike isoform holoʻokoʻa, quantification, PCR bias.
Hoʻokaʻawale ʻo Nanopore sequencing iā ia iho mai nā pae hoʻonohonoho ʻē aʻe, no ka mea, heluhelu pololei ʻia nā nucleotides me ka ʻole o ka DNA synthesis a hoʻopuka i ka heluhelu lōʻihi ma nā ʻumi kilobases.Hāʻawi kēia i ka heluhelu pololei ʻana i nā transcript lōʻihi a me ka hoʻoponopono ʻana i nā pilikia i nā haʻawina isoform-level.
Papahana：Nanopore PromethION
Hale Waihona Puke:cDNA-PCR
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ʻO ke kaʻina mRNA lōʻihi piha -PacBio
De novoka lōʻihi transcriptome sequencing, ʻike ʻia hoʻiDe novoLawe ʻo Iso-Seq i nā pōmaikaʻi o PacBio sequencer i ka lōʻihi heluhelu, e hiki ai i ke kaʻina o nā molekala cDNA piha piha me ka ʻole o ka haki.Hōʻalo loa kēia i nā hewa i hana ʻia i nā ʻanuʻu hui transcript a kūkulu i nā pūʻulu unigene me ka hoʻonā isoform-level.Hāʻawi kēia unigene set i ka ʻike genetic ikaika e like me "genome reference" ma ka pae transcriptome.Eia hou, hui pū me ka ʻikepili sequencing o ka hanauna e hiki mai ana, hāʻawi kēia lawelawe i ka helu kūpono o ka ʻōlelo isoform-level.
Papahana: PacBio Sequel IIHale Waihona Puke: SMRT bele waihona -
Eukaryotic mRNA Sequencing-Illumina
Hiki i ka mRNA sequencing ke hoʻopili i nā mRNA a pau i unuhi ʻia mai nā cell ma lalo o nā kūlana kikoʻī.He ʻenehana ikaika ia no ka hōʻike ʻana i ka moʻolelo hōʻike gene, nā ʻano hana a me nā mīkini molekala o kekahi mau kaʻina olaola.I kēia lā, ua hoʻohana nui ʻia ka mRNA sequencing i ka noiʻi kumu, nā diagnostics clinical, development drug, etc.
Papahana: Illumina NovaSeq 6000
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Non-Reference based mRNA Sequencing-Illumina
Hoʻohana ʻo mRNA sequencing i ke ʻano hana sequencing hanauna hou (NGS) e hopu i ka messenger RNA(mRNA) form Eukaryote i ka manawa kikoʻī e hoʻāla ana kekahi mau hana kūikawā.ʻO ka transcript lōʻihi loa i hoʻopili ʻia ua kapa ʻia ʻo 'Unigene' a hoʻohana ʻia e like me ke kaʻina kuhikuhi no ka nānā ʻana ma hope, ʻo ia ke ala kūpono e aʻo ai i ka mīkini molekala a me ka ʻoihana hoʻoponopono o ka ʻano me ka ʻole o ka kuhikuhi.
Ma hope o ka hōʻuluʻulu ʻana o ka ʻikepili transcriptome a me ka annotation hana unigene
(1) E hoʻokumu mua ʻia ka nānā ʻana o ka SSR, ka wānana CDS a me ke ʻano gene.
(2) E hana ʻia ka helu o ka ʻōlelo unigene i kēlā me kēia laʻana.
(3) E ʻike ʻia nā unigenes i hōʻike ʻia ma waena o nā laʻana (a i ʻole nā hui) ma muli o ka ʻōlelo unigene.
(4) E hoʻokō ʻia ka Clustering, ka wehewehe hana a me ka hoʻonui ʻana i nā unigenes i hōʻike ʻia.
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Ka lōʻihi non-coding sequencing-Illumina
ʻO nā RNA lōʻihi ʻole-coding (lncRNAs) he ʻano molekole RNA me ka lōʻihi ma mua o 200 nt, kahi i hōʻike ʻia e ka haʻahaʻa haʻahaʻa loa.ʻO LncRNA, ma ke ʻano he lālā koʻikoʻi i nā RNA non-coding, ʻike nui ʻia i ka nucleus a me ka plasma.ʻO ka hoʻomohala ʻana i ka ʻenehana sequencing a me nā bioinformtics hiki ke ʻike i nā lncRNA hou a hoʻopili i nā mea me nā hana biological.Hōʻike nā hōʻike hōʻuluʻulu e pili nui ana ka lncRNA i ka hoʻoponopono epigenetic, ka hoʻoponopono transcription a me ka hoʻoponopono post-transcription.
