Ke Kaʻina Metagenomic -NGS
ʻO ka metagenome kahi hōʻiliʻili o ka nui o nā mea genetic o kahi kaiāulu hui o nā meaola, e like me nā metagenomes kaiapuni a me ke kanaka. Loaʻa iā ia nā genomes o nā microorganisms hiki ke kanu a kanu ʻole ʻia. Hiki i ka Shotgun metagenomic sequencing me NGS ke aʻo ʻana i kēia mau ʻāina genomic paʻakikī i hoʻokomo ʻia i loko o nā laʻana kaiapuni ma o ka hāʻawi ʻana i nā mea ʻoi aku ma mua o ka taxonomic profiling, me ka hāʻawi ʻana i nā ʻike kikoʻī i nā ʻano ʻano like ʻole, ka nui o ka dynamics, a me nā hale paʻakikī. Ma waho aʻe o nā haʻawina ʻauhau, hāʻawi pū ka metagenomics shotgun i kahi hiʻohiʻona genomics hana, hiki ke ʻimi i nā ʻano genes i hoʻopaʻa ʻia a me kā lākou hana putative i nā kaʻina kaiaola. ʻO ka mea hope loa, ʻo ka hoʻokumu ʻana i nā ʻupena hoʻopili ma waena o nā mea genetic a me nā mea pili i ke kaiapuni e hāʻawi i ka ʻike holoʻokoʻa o ka pilina paʻakikī ma waena o nā kaiāulu microbial a me ko lākou ʻano kaiaola. I ka hopena, kū ka metagenomic sequencing ma ke ʻano he mea nui no ka wehe ʻana i nā paʻakikī genomic o nā kaiāulu microbial like ʻole, e hoʻomālamalama ana i nā pilina multifaceted ma waena o ka genetics a me ka ecology i loko o kēia mau kaiaola paʻakikī.
Nā papahana: Illumina NovaSeq a me DNBSEQ-T7
Pono lawelawe
●Hoʻokaʻawale a mahiʻai ʻole no ka Microbial Community Profiling: E hiki ai ke kaʻina ʻana o nā mea moʻo mai nā mea ola ʻole.
●Hoʻoholo Kiʻekiʻe: E ʻike i nā ʻano ʻano haʻahaʻa haʻahaʻa ma nā laʻana kaiapuni.
●ʻIkepili Bioinformatics piha:ʻAʻole i nānā wale i ka ʻokoʻa taxonomic akā i ka ʻokoʻa hana o ke kaiāulu.
●ʻIke Nui:Me kahi moʻolelo o ka hoʻopau kūleʻa ʻana i nā papahana metagenomics he nui i nā kikowaena noiʻi like ʻole a me ka hoʻoili ʻana ma luna o 200,000 mau laʻana, lawe kā mākou hui i ka waiwai o ka ʻike i kēlā me kēia papahana.
Nā kikoʻī lawelawe
| Anuu hoʻonohonoho | Hoʻolālā Sequencing | Manaʻo ʻia ka ʻikepili | Ka hoomalu maikai |
| ʻO Illumina NovaSeq a i ʻole DNSSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Nā Koina lawelawe
| Hoʻopaʻa (ng/µL) | huina nui (ng) | Volume (µL) |
| ≥1 | ≥30 | ≥20 |
● Ka lepo / ka lepo: 2-3g
● Maʻiʻo ʻōpū-holoholona: 0.5-2g
● Nā mea i loko o ka ʻōpū-insect: 0.1-0.25g
● ʻili o nā mea kanu (sediment waiwai): 0.5-1g
● Fermentation broth enriched sediment): 0.2-0.5g
● Faeces (holoholona nui): 0.5-2g
● Faeces (iole): 3-5grains
● Pulmonary alveolar holoi holoi: pepa kānana
● ʻAi ʻāʻī: 5-6 swabs
● ʻili/ ʻāʻī ʻāʻī / kohu / waha palupalu / pharyngeal swab / rectal swab: 2-3 swabs
● ʻO nā microorganism o ka ʻili: 5-6 swabs
● Kino wai/ea/biofilm: pepa kānana
● Endophytes: 2-3g
● Papa niho: 0.5-1g
Holo Hana Hana
Hāʻawi laʻana
Kūkulu hale waihona puke
Ke kaʻina ʻana
ʻIkepili ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
Loaʻa i kēia ka nānā ʻana:
● Ka hoʻomalu ʻana i ka maikaʻi o ka ʻikepili
● Hui Metagenome a me ka wānana gene
● Hōʻike Gene
● Kānāwai ʻokoʻa alpha taxonomic
● Ka nānā 'ana o ke kaiāulu: ka hana biological, metabolic, antibiotic resistance
● Ka nānā 'ana i ka 'oko'a hana a me ka 'auhau:
ʻIkepili ʻokoʻa Beta
ʻIkepili waena
Ka nānā 'ana i ka pilina: ma waena o nā mea kaiapuni a me ka haku mele 'ana a me ka 'oko'a
Nānā hana: CARD antibiotic resistance
ʻO ka nānā ʻana i nā alahele metabolic KEGG: heatmap o nā ala koʻikoʻi
Ka ʻokoʻa Alpha o ka puʻunaue ʻauhau: ACE index
Ka ʻokoʻa Beta o ka mahele ʻauhau: PCoA
E ʻimi i nā holomua i hoʻokō ʻia e nā lawelawe hoʻokaʻina metagenome a BMKGene me Illumina ma o ka hōʻiliʻili ʻana o nā puke.
Hai, Q. et al. (2023) 'Metagenomic a me ka metabolomic analysis o nā loli i loko o ka ʻōpū o ke ānuenue trout (Oncorhynchus mykiss) i hoʻopaʻa ʻia me ka maʻi ʻeha hematopoietic necrosis ma nā ʻano wela o ka wai moʻomeheu',Nā palena i ka Microbiology, 14, p. 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. et al. (2023) 'Nā kaiaulu microbial, nā genes kū'ē, a me nā pilikia kū'ē i nā loko kūlanakauhale o nā moku'āina trophic like'ole: Nā loulou i loko a me nā hopena o waho',Nūpepa o nā mea pilikia, 9, p. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Hōʻike ʻia ka ʻike ʻana o Metagenomic i nā ʻokoʻa o ka hoʻohui ʻana a me ka hana ma waena o nā ʻano microorganisms pili i ka wai a me nā mea paʻa o ka hipa Rumen',Nā palena i ka Microbiology, 13, p. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Obese Ningxiang puaʻa microbiota rewires carnitine metabolism no ka paipai ʻana i ka hoʻoheheʻe ʻana o ka momona momona i nā puaʻa DLY lean',Ka Hana Hou, 4(5), p. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Nāʻike metagenomic i nā pilikia e hiki mai ana o nā mea bio/non-degradable plastic a me nā ʻōpala non-plastic ma luna a me lalo o Haihe Estuary, Kina',ʻEpekema o Ka Kaiapuni Hui Pū ʻIa, 887, p. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
