E komo i loko o BMKCloud

Nā huahana

Ka Hoʻonohonoho ʻana o ka DNA/RNA - Nanopore Sequencer

Ka Hoʻonohonoho ʻana o ka DNA/RNA - Kiʻi i Hōʻike ʻia o ka Nanopore Sequencer

ʻO ka hoʻonohonoho ʻana o ONT kahi ʻenehana hoʻonohonoho hōʻailona uila manawa maoli o hoʻokahi molekala e pili ana i nā nanopores, like ke kumumanaʻo o kēlā me kēia kahua. E hoʻopaʻa ka DNA/RNA pālua i ka protein nanoporous i hoʻokomo ʻia i loko o ka biofilm a wehe ʻia ma lalo o ke alakaʻi o ka protein motor, ma lalo o ka hana o ka ʻokoʻa voltage mai nā ʻaoʻao ʻelua o ka biofilm, hele nā kaula DNA/RNA ma o ka protein nanopore channel ma kahi wikiwiki. Ma muli o nā ʻokoʻa o nā waiwai kemika o nā kumu like ʻole ma ke kaula DNA/RNA, ke hele kahi kumu hoʻokahi a i ʻole ka mole DNA ma o ke kahawai nanopore, e hoʻololi ia i nā hōʻailona uila like ʻole. Ma ka ʻike ʻana a me ka pili ʻana i kēia mau hōʻailona, hiki ke helu ʻia nā ʻano kumu like, a hiki ke hoʻopau ʻia ka ʻike ʻana i ka manawa maoli o ke kaʻina.

Kāhea iā mā˚ou

Nā kikoʻī lawelawe

Hopena Hōʻike

Nā hiʻohiʻona kikoʻī lawelawe

Paepae	Ka nui o ka hale waihona puke	Ka Hua ʻIkepili Manaʻo (No kēlā me kēia Kelepona)	Pololei kumu hoʻokahi	Nā noi
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Kelepa	85-92%	Kāhea ʻana o SV, De novo, Kaʻina hana piha, Iso-Seq, Ka hoʻākāka ʻana o ka gene, Ka ʻike ʻana i ka methylation DNA

Nā Pōmaikaʻi o ka Lawelawe

● Ma mua o 5 mau makahiki o ka ʻike ma ka paepae hoʻonohonoho PacBio me nā tausani o nā papahana pani ʻia me nā ʻano like ʻole.
● He hoa kūhelu ʻo BMKGENE no Oxford Nanopore, me ka palapala hōʻoia RNA/DNA paepae pālua.
● Aia nā hiʻohiʻona koʻikoʻi o nā sequencers me nā lako piha a me ka lawa ʻana o ka sequencing.
● Ma muli o ka paepae Nanopore, ua paʻi ʻia ma mua o 10 mau noiʻi holoholona a me nā mea kanu Denovo ma nā puke pai kaulana o ka honua.

Nā Koina Laʻana

ʻAno Laʻana

Ka nui

Ka Hoʻohuihui (Qubit ®)

Leo

Maʻemaʻe

ʻO kekahi poʻe

ʻADN genomic

E hilinaʻi i ke koi ʻikepili

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

ʻO ka piko maopopo ma 260 nm, ʻaʻohe haumia

Pono e ana ʻia ka noʻonoʻo ʻana e Qubit a me Qubit/Nanopore ≤ 2

RNA holoʻokoʻa

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5; ʻaʻohe haumia

Ka waiwai RIN ≥7.5

Kaʻina Hana Lawelawe

Hoʻomākaukau laʻana

Ke kūkulu ʻana i ka hale waihona puke

Ka hoʻonohonoho ʻana

Ka loiloi ʻikepili

Ka hoʻouna ʻana o ka papahana

Ma mua: Nā hale waihona puke i hana mua ʻia ʻo Illumina

Aʻe: Ka Hoʻonohonoho ʻĀpana Hoʻonui ʻia o ka Locus Kūikawā (SLAF-Seq)

Loiloi Kūlana ʻIkepili o ka Laʻana DNA

Papa 1. Nā helu helu ma ka ʻikepili maʻemaʻe.

BMKID	makaSeqNum	kumu kūʻai rawSum	helu hoʻomaʻemaʻe	maʻemaʻeSumBase	maʻemaʻeN50Len	maʻemaʻeN90Len	maʻemaʻeMeanLen	maʻemaʻeMaxLen	maʻemaʻeMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9