Dna / rna seequing - nanopore sequencer
Nā hiʻohiʻona kiko'ī
| Kahua | Palapala waihona | ʻO ka hōʻikeʻikepili Pūnaewele (ma kēlā me kēia cell) | ʻO ka pololei hoʻokahi | Noi |
| Nana'Ooke | 8KB, 10KB, 20kb, ultralong, cdna-PCR | 70-90BBS / CELL | 85-92% | SV Kāhea, de novo, piha piha, piha, iso-seq |
Loaʻa nā lawelawe lawelawe
● Ma luna o 5 mau makahiki 'i nā makahiki ma Pacbio Sequence Stangincing Platform me nā kaukani o nā hana i paniʻia me nāʻano likeʻole.
● He hoa hanaʻo BMKGEE o Oxford Nanopore, me nā wahiʻelua rna / dna hōʻoia.
● He mau hiʻohiʻona nui o nā hiʻohiʻona o nā papa hana me nā lako hana piha a kūpono i ka hoʻokomoʻana.
● E pili ana i ka Platform Nanopore,ʻoi aku ma mua o 10 mau holoholona a me nā mea kūʻai aku a me nā mea kūʻai akuʻo Denovo.
Nā koi koi
| ʻAno hoʻohālike | Ka nui | Concerttion (Qonbit ®) | Volume | ʻO Menu ka hoʻomaʻemaʻe | Maahua |
| Genomam dNA | E hilinaʻi i ka pono o kaʻikepili | ≥20ng / μl | ≥15μl | Od260 / 280 = 1.7-2.2; Od260 / 230≥.5; Hoʻomaʻamaʻaʻo Peak ma 260 NM,ʻaʻohe mea hilahila | Pono eʻaeʻia e ka nīnauʻana e ka nīnau a me ka nīnau / nanopore ≤ 2 |
| Huina rna | ≥1.2μg | ≥100μg / μl | ≥15μl | Od260 / 280 = 1.7-2.5; Od260 / 230 = 0.5-2.5;ʻaʻohe mea hoʻohālikelike | Rin waiwai ≥7.5 |
Haʻawina lawelawe
Mākaukau hoʻomākaukau
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Nā Hana Hana
ʻO ka loiloiʻikepiliʻikepili o DNA DNA
Papa 1. Nā helu helu ma kaʻikepili maʻemaʻe.
| BMKID | rawaqseqnum | rawsbrease | hoʻomaʻemaʻe | hoʻomaʻemaʻe | maʻemaʻe | windan90n | maʻemaʻekaha | emae 的 | maʻemaʻe |
| DNA_BMK01 | 1,218,239 | 26.37 | 1,121,736 | 25.90 | 28,014 | 15,764 | 23,090 | 143,181 | 9 |
ʻO ka loiloi maikaʻi o kaʻikepili o RNA
Papa 1. Nā helu helu ma kaʻikepili maʻemaʻe.
| Filename | Lunauhau | Heluhelu | Baseal | N50 | Misslenting | Kūpono Kaawe | Heinonsce |
| Rna_bmk001 | C2 | 8,947,708 | 4,047,230,083 | 398 | 452 | 129,227 | Q12 |
Kiʻi 1. E heluhelu i ka helu lōʻihi
Kiʻi 2.ʻO ka heluʻana o ka helu helu o kaʻike o kaʻike maʻemaʻe
Kiʻi 3.
