GENOME EVOLUTION
xwm
Kev sib txuas lus
genome sequences qhia thoob ntiaj teb kev faib tawm txoj hauv kev thiab qhia txog kev hloov pauv caj ces hloov pauv hauv seahorse evolution
PacBio |Illumina |Hi-C |WGS |Genetic Diversity |Keeb Kwm Keeb Kwm |Gene Flow
Pacbio sequencing, genome de novo los ua ke thiab annotation cov kev pabcuam tau muab los ntawm Biomarker Technologies.
Qhov tseem ceeb
1.A zoo chromosome-theem seahorse (Hippocampus erectus) genome nrog contig N50 ntawm 15.5 Mb tau txais.
2.A tag nrho ntawm 358 genomes los ntawm thoob ntiaj teb 21 hom seahorse tau rov ua dua.
3.Seahorses hloov zuj zus nyob rau hauv lub lig Oligocene thiab tom ntej no circum-global colonization txoj kev raug txheeb xyuas thiab txuas mus rau kev hloov dynamics nyob rau hauv dej hiav txwv tam sim no thiab paleotemporal seaway qhib.
4.Lub hauv paus caj ces ntawm qhov rov tshwm sim "pob txha pob txha" hloov phenotype yog txuas rau kev hloov pauv ywj pheej hauv cov noob caj noob ces tseem ceeb.
5.Rrafting ntawm dej hiav txwv tam sim no them nyiaj rau cov neeg tawg rog tsis zoo thiab kev hloov pauv sai sai ua rau muaj kev thaj yeeb nyab xeeb tshiab
Kev ua tiav
Daim duab 1 Genetic diversity thiab phylogenetic kev sib raug zoo ntawm 358 seahorse specimens
aGeographic sampling qhov chaw rau cov qauv seahorses nrog cov qauv ntawm nucleotide ntau haiv neeg (π) ntawm 21 hom seahorse hla 22 chromosomes.b Neighborjoining ntoo tsim nrog genome-wide SNPs ntawm 358 seahorses.Qhov chaw tus pin cim hauv (a) thiab ceg keeb kwm yav dhau hauv (b) sib raug rau ib leeg.
Daim duab 2 Colonization thiab pej xeem keeb kwm ntawm seahorses
aPhylogenetic ntoo thiab divergence lub sij hawm kwv yees rau 21 hom seahorse.Cov ceg kab tuab sib haum rau cov pej xeem qhov kwv yees (Ne) thiab xim qhia cov kab sib txawv.Cov cim I-III qhia cov ntsiab lus calibration.b–d Kev kwv yees txoj kev colonization (cov xub xim xim) ntawm seahorses raws li lub sij hawm sib txawv, kev faib tawm, cov xwm txheej vicariance, thiab dej hiav txwv tam sim no (dawb xub).b Indo-Australian Archipelago yog qhov chaw ntawm keeb kwm (xim liab) ntawm genus Hippocampus ua ntej seahorses diversified thiab dispersed thoob ntiaj teb 18-23 Ma.c Seahorses pib colonized Dej Hiav Txwv Atlantic los ntawm kev qhib Tethyan seaway, uas, tom qab nws kaw (Terminal Event thaum lub sij hawm 7–13 Ma), cais Tethyan kab no los ntawm nws cov kwv tij Indian dej hiav txwv.Tom qab ntawd, tom qab sib txawv sai heev (daj cim) nyob rau hauv Arabian Hiav Txwv, tsim ib tug thib ob qhov chaw ntawm seahorse diversification.d Qhov thib ob seahorse colonization tshwm sim ntawm Dej Hiav Txwv Atlantic tau tshwm sim los ntawm Dej Hiav Txwv Indian txog 5Ma los ntawm kev hla South African tip, thiab thaum kawg tuaj txog rau sab hnub tuaj Pacific Dej hiav txwv los ntawm qhov tseem qhib Panama seaway kwv yees li 3.6 Ma.
Fig. 3 Gene flow thiab fluctuations in the effective population size
aGene ntws kuaj pom ntawm cov tsiaj nyob hauv South Atlantic Dej hiav txwv.Gene flow yog qhia nyob ze cov kab dawb raws li kev tsiv teb tsaws chaw txiav tawm los ntawm G-PhoCS.Thickness thiab kev taw qhia ntawm cov xub sib raug rau cov nqi thiab cov kev taw qhia ntawm cov noob ntws, feem.b Kev hloov pauv ntawm cov pej xeem zoo los ntawm PSMC.Lub x axis sawv cev rau lub sijhawm xyoo ua ntej tam sim no thaum lub y axis sawv cev rau cov pejxeem loj.Cov kab kos tau teeb tsa feem ntau raws li thaj chaw faib khoom rau txhua hom nrog thaj chaw sib txawv.c Hiav txwv theem hloov thaum 1 lab xyoo dhau los hauv metres33.Cov kab daj qhia qhov kawg ntawm lub ntiaj teb interglacial ncov thaum lub cyan ntxoov ntxoo qhia lub sijhawm glacial siab kawg.
Fig. 4 Cov evolution ntawm qaum.
aSab laug, Tsiaj tsob ntoo nthuav tawm txoj kev ywj pheej ntawm cov spines hauv seahorses.Cov ceg ntev qhia tias muaj pes tsawg tus hloov pauv ib qhov chaw.Plaub hom kab seahorse tseem ceeb hauv xiav.Thicker ceg sib haum mus rau cov nqi siab dua ntawm nonsynonymous-to-synonymous hloov pauv (dN/dS) rau bmp3 noob.Canonical thiab generalized McDonald thiab Kreitman test (MKT) rau bmp3 noob tau ua rau peb hom viv ncaus uas muaj qhov sib txawv spiny thiab tsis-spiny nta tseem ceeb los ntawm cov xim tom qab, nws qhov tseem ceeb theem tau qhia los ntawm p tus nqi nrog xiav thiab liab font, ntsig txog.Txoj cai, kev sib piv ntawm cov amino acid hloov pauv hauv bmp3 protein, polymorphic thiab ruaj hloov pauv hauv spiny seahorses yog qhia nrog lub voj voog liab thiab xiav, feem.b Kev faib tawm ntawm dN / dS qhov tseem ceeb hauv bmp3 hauv spiny seahorses piv rau hom tsis spiny.c Kev ywj pheej evolution nyob rau hauv tsob ntoo phylogenetic reconstructed rau cov protein encoded los ntawm bmp3.d Tag nrho-mount hauv situ hybridization ntawm bmp3 hauv Hippocampus erectus.
Kev siv
Li C et al.genome sequences qhia thoob ntiaj teb kev faib tawm txoj hauv kev thiab qhia txog kev hloov pauv caj ces hloov pauv hauv seahorse evolution.Nat Commun.2021 Peb 17; 12(1): 1094.
Xov xwm thiab qhov tseem ceeb Lub hom phiaj ntawm kev sib koom cov xwm txheej ua tiav tshiab nrog Biomarker Technologies, khaws cov kev tshawb fawb tshiab thiab cov txuj ci tseem ceeb siv thaum kawm.
Post lub sij hawm: Jan-06-2022