I-Metagenomics (NGS)

I-Shotgun metagenomics ene-Illumina iyithuluzi elidumile lokufunda ama-microbiomes ngokulandelanisa ngokuqondile i-DNA evela kumasampuli ayinkimbinkimbi, okuvumela ukucwaninga kokubili kwe-taxonomic nokusebenza okuhlukahlukene. Ipayipi le-BMKCloud metagenomic (NGS) liqala ngokulawulwa kwekhwalithi kanye nokuhlanganisa i-metagenome, lapho izakhi zofuzo zibikezelwa futhi zihlanganiswe zibe amasethi edatha angasadingeki achazelwe umsebenzi kanye ne-taxonomy kusetshenziswa imininingwane eminingi. Lolu lwazi lusetshenziselwa ukuhlaziya ngaphakathi kwesampula ukwehlukahlukana kwe-taxonomic (ukwehlukahlukana kwe-alpha) naphakathi kwesampula ukuhlukahluka (ukuhlukahluka kwe-beta). Ukuhlaziywa okuhlukile phakathi kwamaqembu kuthola ama-OTU kanye nemisebenzi yebhayoloji ehluka phakathi kwamaqembu amabili esebenzisa kokubili ukuhlola kwepharamethrikhi nokungeyona eyepharamitha, kuyilapho ukuhlaziywa kokuhlobanisa kuhlobanisa lo mehluko nezici zemvelo.