I-Metagenomics (NGS)

I-Shotgun metagenomics ene-Illumina iyithuluzi elidumile lokufunda ama-microbiomes ngokulandelelanisa ngqo i-DNA kumasampula ayinkimbinkimbi, okwenza kube lula ukutadisha kokubili ukuhlukahluka kwe-taxonomic kanye nokusebenza. Ipayipi le-BMKCloud metagenomic (NGS) liqala ngokulawula ikhwalithi kanye nokuhlanganiswa kwe-metagenome, lapho izakhi zofuzo zibikezelwa khona futhi zihlanganiswe zibe amasethi edatha angewona aphindaphindayo achazwa ngomsebenzi kanye ne-taxonomy kusetshenziswa izizindalwazi eziningi. Lolu lwazi lusetshenziselwa ukuhlaziya ukuhlukahluka kwe-taxonomic ngaphakathi kwesampula (ukuhlukahluka kwe-alpha) kanye nokuhlukahluka kwesampula phakathi (ukuhlukahluka kwe-beta). Ukuhlaziywa komehluko phakathi kwamaqembu kuthola ama-OTU kanye nemisebenzi yezinto eziphilayo ehlukene phakathi kwamaqembu amabili kusetshenziswa kokubili ukuhlolwa kwe-parametric kanye nokungewona kwe-parametric, kuyilapho ukuhlaziywa kokuxhumana kuhlobanisa lokhu kuhluka nezici zemvelo.