Ukuhlaziywa kwe-mRNA ye-Eukaryotic (Izinketho ezisekelwe ezireferensini kanye nezinketho ezintsha ezitholakalayo)
Lo mzila usebenzisa idatha ye-NGS RNA-Seq njengokufakwayo futhi ukhiqiza imiphumela evela ekuhlaziyweni okuningi okungezansi okuhlanganisa kodwa kungagcini lapho: ukulandelana kokuhlolwa kwekhwalithi yedatha,de novoisichasiselo sendawo yokubhala umbhalo, ukuhlaziywa kokuhlanganisa okuguquguqukayo, ukuhlaziywa kokubonakaliswa komehluko, isichasiselo somsebenzi kanye nokuhlaziywa kokunotha.
Ukuhlaziywa kwe-RNA okungeyona i-coding ende
Ama-RNA amade angabhalisi (i-lncRNA) ayimibhalo engabhalisi enobude obungaphezu kwama-200 nt futhi aziwa ngokudlala indima ekuhleleni nasekulawuleni i-chromatin. Ubuchwepheshe bokulandelana okuphezulu kanye ne-bioinformatic kunikeze amandla okuqonda kwethu ukulandelana kwe-lncRNA kanye nolwazi lokubeka ukuze kutholakale ama-lncRNA anemisebenzi ebalulekile yokulawula. Lo mzila uhlinzeka ngokuhlaziywa kwe-lncRNA ngaphezu kokuhlaziywa okukhulunywe ngakho kumzila wokuhlaziya i-mRNA we-Eukaryotic.
Ukulandelana kwe-amplicon ye-16S/18S/ITS
Ipayipi lokuhlaziya ukuhlukahluka kwamagciwane e-Amplicon Sequencing lakhiwe ngokusekelwe eminyakeni yokuhlangenwe nakho ekuhlaziyweni kwephrojekthi yokuhlukahluka kwamagciwane. Leli payipi liqukethe ukuhlaziywa okuyisisekelo okujwayelekile, okuhlanganisa okuqukethwe kokuhlaziywa okujwayelekile kocwaningo lwamanje lwamagciwane kanye nokuhlaziywa komuntu siqu. Umbiko wokuhlaziya ucebile futhi uphelele, kanye nenketho yokwenza ukuhlaziywa komuntu siqu okuhlukahlukene. Ngaphezu kwalokho, amasampula namaqembu angashintshwa ngokushesha ukuze kwenziwe ngokwezifiso nokulawula okwengeziwe.
Ukuhlaziywa kwe-Shotgun Metagenomics
Ipayipi Lokuhlaziywa Kwe-Shotgun Metagenomic lisebenzisa idatha ye-NGS evela ezintweni ezixubile ze-genomic ezikhishwe kumasampula ezemvelo. Ukuhlaziywa okufakiwe kunikeza ulwazi oluningiliziwe ngokuhlukahluka kwezinhlobo kanye nobuningi bazo, isakhiwo sabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo, kanye namanethiwekhi okuhlobana nezici zemvelo.
Ukuhlaziywa Kokuhlukahluka kwe-NGS-WGS
Ukuhlaziywa Kokuhlukahluka kwe-NGS-WGS kuyipayipi lokuthola okuhlukahlukene elihlanganisiwe elenza ukulawulwa kwekhwalithi yedatha, ukuvumelanisa ukulandelana, isichasiselo, kanye nokuhlaziywa kokuguqulwa kwezakhi zofuzo. Leli payipi lilandela imikhuba emihle ye-GATK yokuthola i-SNP kanye ne-InDel futhi lisebenzisa i-Manta ekubizeni okuhlukile kwesakhiwo.
Ucwaningo Lwenhlangano Ebanzi Ye-Genome (i-GWAS)
Iphayiphi ye-GWAS iwukuhlaziywa okungezansi okuthatha amafayela e-VCF akhiqizwe ngaphambilini kanye nedatha ye-phenotype ehambisanayo yeqembu labantu njengokufakwayo. Isebenzisa izindlela ezithile zezibalo, i-GWAS ihlose ukuthola ukwehluka kwe-nucleotide ye-genome ehlobene nokwehluka kwe-phenotypic. Idlala indima ebalulekile ekuhloleni izakhi zofuzo ezisebenzayo ezihlotshaniswa nezifo eziyinkimbinkimbi zabantu kanye nezici eziyinkimbinkimbi ezitshalweni nasezilwaneni.
Ukuhlaziywa Kokuhlukaniswa Okubanzi (i-BSA)
Ukuhlaziywa kwe-BSA kuhilela ukuhlanganisa abantu abanezimpawu ze-phenotypic ezingavamile kubantu abahlukanisayo. Ngokuqhathanisa izindawo ezihlukile phakathi kwamasampula ahlanganisiwe, le ndlela ikhomba ngokushesha izimpawu zama-molecule ezihlobene eduze ezihlotshaniswa nezakhi zofuzo eziqondiwe. Isetshenziswa kabanzi ekudwebeni izakhi zofuzo zezitshalo nezilwane, iyithuluzi eliwusizo lokuzalanisa ngosizo lwezimpawu.
Ukuhlaziywa Kwezakhi Zofuzo Zokuziphendukela Kwemvelo
Ukuhamba komsebenzi kwe-Evolutionary Genetics Analysis kusebenzisa ulwazi olubanzi lwe-BMKGENE kumaphrojekthi okuziphendukela kwezakhi zofuzo futhi kufaka phakathi ukwakhiwa kwesihlahla se-phylogenetic, ukuhlaziywa kwe-linkage disequilibrium, ukuhlolwa kokuhlukahluka kwezakhi zofuzo, ukuhlonza okukhethiwe, ukuhlaziywa kobuhlobo, ukuhlaziywa kwezingxenye eziyinhloko, kanye nokuhlukaniswa kwesakhiwo sabantu.
