i-mRNA-seq (NGS) ene-genome ebhekisele kuyo
I-RNA-seq iyithuluzi elijwayelekile kuzo zonke isayensi yempilo nezitshalo, ivala igebe phakathi kwama-genome nama-proteome. Amandla ayo atholakala ekutholeni imibhalo emisha kanye nokulinganisa ukuvezwa kwawo ngokuhlolwa okukodwa. Isetshenziswa kabanzi ezifundweni zokuqhathanisa ze-transcriptomic, ikhanyisa izakhi zofuzo ezihlobene nezici noma ama-phenotype ahlukahlukene, njengokuqhathanisa i-mutant nezinhlobo zasendle noma ukuveza ukubonakaliswa kwezakhi zofuzo ngaphansi kwezimo ezithile. I-BMKCloud mRNA(Reference) APP ihlanganisa ukulinganiswa kokubonakaliswa, ukuhlaziywa kokubonakaliswa kokungafani (DEG), kanye nokuhlaziywa kwesakhiwo sokulandelana kupayipi le-mRNA-seq(NGS) bioinformatics futhi ihlanganisa amandla esofthiwe efanayo, iqinisekisa ukusebenziseka kalula kanye nobungane bomsebenzisi. Abasebenzisi bangalayisha idatha yabo ye-RNA-seq efwini, lapho i-App inikeza khona isisombululo sokuhlaziya se-bioinformatic esiphelele, esisodwa. Ngaphezu kwalokho, ibeka phambili ulwazi lwamakhasimende, inikeza imisebenzi eyenzelwe wena ngokwezidingo ezithile zabasebenzisi. Abasebenzisi bangasetha amapharamitha futhi bathumele umsebenzi wepayipi ngokwabo, bahlole umbiko osebenzisanayo, babuke idatha/imidwebo kanye nokumbiwa kwedatha okuphelele, njengokuthi: ukukhethwa kwezakhi zofuzo okuqondiwe, ukuhlanganiswa okusebenzayo, ukudweba, njll.
Ipulatifomu:I-Illumina, MGI
Isu:I-RNA-Seq
Isakhiwo: Idatha ehlanzekile, ehlanzekile.
Uhlobo lwelabhulali:i-fr-unstranded, i-fr-firststrand noma i-fr-secondstrand
Ubude bokufunda:150 bp
Uhlobo lwefayela:*.fastq, *.fq, *.fastq.gz noma *.fq.gz. Uhlelo luzokwenzahlanganisa ngokuzenzakalelayo amafayela e-.fastq ngokuya ngamagama amafayela awo,isib. *_1.fastq ihlanganiswe ne *._2.fastq.
Inani lamasampula:Akukho mingcele enaninikwamasampula, kodwa isikhathi sokuhlaziya sizokhula njengoba inaniamasampula ayakhula.
Inani Ledatha Elinconywayo:6G ngesampula ngayinye
