Ukuhlanganiswa Kwe-Genome Okusekelwe ku-Hi-C
I-Hi-C iyindlela eklanyelwe ukubamba ukumiswa kwe-chromosome ngokuhlanganisa ukusebenzisana okusekelwe ekuhloleni ukusondelana kanye nokulandelana okuphezulu. Ukuqina kwalokhu kusebenzisana kukholakala ukuthi kuhlobene kabi nebanga elingokoqobo kuma-chromosome. Ngakho-ke, idatha ye-Hi-C isetshenziselwa ukuqondisa ukuhlanganiswa, ukuhleleka, kanye nokuqondiswa kokulandelana okuhlanganisiwe ku-genome ehleliwe kanye nokunamathela kunani elithile lama-chromosome. Lobu buchwepheshe bunika amandla ukuhlanganiswa kwe-genome yezinga le-chromosome lapho kungekho imephu yezakhi zofuzo esekelwe kubantu. I-genome ngayinye idinga i-Hi-C.
Izici Zesevisi
● Ukulandelana kwe-Illumina NovaSeq nge-PE150.
● Isevisi idinga amasampula ezicubu, esikhundleni sama-nucleic acid akhishwe, ukuze axhumane ne-formaldehyde futhi alondoloze ukusebenzisana kwe-DNA-protein.
● Ukuhlolwa kwe-Hi-C kuhilela ukuvinjelwa nokulungiswa kokuphela kweziphetho ezinamathelayo nge-biotin, kulandelwe ukujikeleziswa kweziphetho ezibuthuntu eziphumayo ngenkathi kulondolozwa ukusebenzisana. I-DNA ibe isidonswa phansi ngama-streptavidin beads bese ihlanzwa ukuze kulungiselelwe umtapo wolwazi olandelayo.
Izinzuzo Zesevisi
Ukubuka konke kwe-Hi-C
(Lieberman-Aiden E et al.,Isayensi, 2009)
●Ukuqeda Isidingo Sedatha Yabantu Abasebenzisa Izakhi Zofuzo:I-Hi-C ithatha indawo yolwazi olubalulekile oludingekayo ekuhlanganiseni i-anchor.
●Ubuningi bezimpawu eziphezulu:okuholela esilinganisweni esiphezulu sokubambelela esihambisanayo esingaphezu kuka-90%.
●Ubuchwepheshe Obubanzi kanye namarekhodi okushicilela:I-BMKGene inolwazi olukhulu ngamacala angaphezu kuka-2000 e-Hi-C Genome Assembly avela ezinhlotsheni ezahlukene eziyi-1000 kanye namalungelo obunikazi ahlukahlukene. Amacala angaphezu kuka-200 ashicilelwe ane-accumulative impact factor engaphezu kuka-2000.
●Ithimba le-bioinformatics elinekhono eliphezulu:ngama-patents angaphakathi kanye namalungelo okushicilela esofthiwe okuhlola kwe-Hi-C kanye nokuhlaziywa kwedatha, isofthiwe yedatha yokubona ethuthukiswe ngokwayo ivumela ukunyakaza, ukuhlehlisa, ukubuyisa, kanye nokwenza kabusha ibhlogo ngesandla.
●Usekelo Lwangemva Kokuthengisa:Ukuzibophezela kwethu kudlulela ngale kokuqedwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi zemibuzo nezimpendulo ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.
●Isichasiselo Esiphelele: sisebenzisa izizindalwazi eziningi ukuze sichaze izakhi zofuzo ngokusebenza ngokuhlukahluka okubonakalayo futhi senze ukuhlaziywa kokunotha okuhambisanayo, sinikeze ukuqonda kumaphrojekthi amaningi ocwaningo.
Imininingwane Yesevisi
| Ukulungiselela umtapo wolwazi | Isu lokulandelana kwezigaba | Ukukhishwa kwedatha okunconyiwe | Ikhwalithi yokulawula |
| Umtapo wolwazi we-Hi-C | I-Illumina NovaSeq PE150 | 100x | Q30 ≥ 85% |
Izidingo Zesampula
| Izicubu | Inani elidingekayo |
| I-Animal Viscera | ≥ 1 g |
| Imisipha Yezilwane | |
| Igazi Lezilwane Ezincelisayo | ≥ 2 mL |
| Igazi Lenkukhu/Lenhlanzi | |
| Isitshalo - Iqabunga Elisha | ≥ 2 g |
| Amaseli Akhuliswe | ≥ 1x107 |
| Isinambuzane | ≥ 1 g |
| Izinto eziphilayo zasemanzini - Izicubu Zemisipha | ≥ 1 g |
| Izinto eziphilayo zasemanzini - Ulwelwe | ≥ 2 g |
Ukugeleza Komsebenzi Wesevisi
Umklamo wokuhlola
Ukulethwa kwesampula
Ukwakhiwa komtapo wolwazi
Ukulandelana
Ukuhlaziywa kwedatha
Izinsizakalo zangemuva kokuthengisa
1) Idatha eluhlaza QC
2) I-QC yomtapo wolwazi we-Hi-C: ukulinganisa ukusebenzisana kwe-Hi-C okusebenzayo
3) Umhlangano we-Hi-C: ukuhlanganiswa kwama-contig ngamaqembu, kulandelwe ukuhleleka kwama-contig ngaphakathi kweqembu ngalinye kanye nokwabela ukuqondiswa kwama-contig
4) Ukuhlolwa kwe-Hi-C
I-Hi-C Library QC – ukulinganiselwa kwama-pair okuxhumana asebenzayo e-Hi-C
I-Hi-C Assembly – izibalo
Ukuhlolwa kwangemva kokuhlanganiswa - imephu yokushisa yokuqina kwesignali phakathi kwamabhini
Hlola intuthuko eyenziwe yizinsizakalo zokuhlanganisa ze-BMKGene ze-Hi-C ngokusebenzisa iqoqo lezincwadi ezikhethiwe.
UTian, T. et al. (2023) 'Ukuhlanganiswa kwezakhi zofuzo kanye nokwahlukaniswa kwezakhi zofuzo ze-germplasm yommbila evelele engamelani nesomiso', i-Nature Genetics 2023 55:3, 55(3), amakhasi 496–506. doi: 10.1038/s41588-023-01297-y.
UWang, ZL nabanye. (2020) 'Ukuhlanganiswa Kwesilinganiso Se-Chromosome Se-Asian Honeybee Apis cerana Genome', I-Frontiers in Genetics, 11, k. 524140. doi: 10.3389/FGENE.2020.00279/BIBTEX.
UZhang, F. nabanye. (2023) 'Ukwembula ukuvela kwe-tropane alkaloid biosynthesis ngokuhlaziya ama-genome amabili emndenini wakwaSolanaceae', Nature Communications 2023 14:1, 14(1), pp. 1–18. doi: 10.1038/s41467-023-37133-4.
UZhang, X. nabanye. (2020) 'Izakhi zofuzo zesihlahla se-Banyan kanye ne-Pollinator Wasp zinikeza ukuqonda nge-Fig-Wasp Coevolution', Cell, 183(4), pp. 875-889.e17. doi: 10.1016/J.CELL.2020.09.043
