I-Illumina Yokulandelanisa Okungeyona ikhodi ende
Izinzuzo Zesevisi
●Ukuhlaziywa okuhlangene kwe-mRNA ne-lncRNA: ngokuhlanganisa i-quantification yemibhalo ye-mRNA nocwaningo lwe-lncRNA kanye nezinhloso zabo, kungenzeka ukuthi uthole ukubuka konke okujulile kwendlela yokulawula engaphansi kwempendulo yeselula.
●Ubungcweti Olubanzi: Ithimba lethu liletha ingcebo yesipiliyoni kuyo yonke iphrojekthi, enomlando wokucutshungulwa kwamasampula angaphezu kuka-23,000 ku-BMK ehlanganisa izinhlobo zamasampula ahlukahlukene kanye namaphrojekthi e-lncRNA.
●Ukulawulwa Kwekhwalithi Okuqinile: Sisebenzisa amaphuzu okulawula abalulekile kuzo zonke izigaba, kusukela kusampula nokulungiselela umtapo wezincwadi ukuya ekulandeleni nasekulandeleni i-bioinformatics. Lokhu kuqapha ngokucophelela kuqinisekisa ukulethwa kwemiphumela ehlala njalo yekhwalithi ephezulu.
●Ukusekelwa Kwangemva Kokuthengisa: Ukuzinikela kwethu kudlulela ngalé kokuphothulwa kwephrojekthi ngenkathi yesevisi yezinyanga ezi-3 ngemva kokuthengisa. Ngalesi sikhathi, sinikezela ngokulandelelwa kwephrojekthi, usizo lokuxazulula inkinga, namaseshini e-Q&A ukuze siphendule noma yimiphi imibuzo ehlobene nemiphumela.
Izidingo Zesampula Nokulethwa
| Umtapowolwazi | Inkundla | Idatha enconyiwe | Idatha ye-QC |
| I-rRNA iqede umtapo wolwazi wokuqondisa | I-Ilumina PE150 | 10-16 Gb | Q30≥85% |
Ama-Nucleotides:
| Conc.(ng/μl) | Inani (μg) | Ubumsulwa | Ubuqotho |
| ≥80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Amaprotheni anomkhawulo noma awekho nhlobo noma ukungcola kwe-DNA okuboniswa kujeli. | I-RIN≥6.0; 5.0≥28S/18S≥1.0; ukuphakama okulinganiselwe noma okungenakho |
● Izitshalo:
Izimpande, Isiqu noma I-Petal: 450 mg
Iqabunga noma Imbewu: 300 mg
Isithelo: 1.2 g
● Isilwane:
Inhliziyo noma Amathumbu: 450 mg
I-Viscera noma Ubuchopho: 240 mg
Imisipha: 600 mg
Amathambo, Izinwele noma Isikhumba: 1.5g
● Ama-arthropods:
Izinambuzane: 9g
I-crustacea: 450 mg
● Igazi eliphelele:2 amashubhu
● Amaseli: 106 amaseli
● I-Serum ne-Plasmaawu :6ml
Ukulethwa Kwesampula Okunconyiwe
Isitsha: 2 ml centrifuge tube (Tin foil ayinconywa)
Ukulebula okuyisampula: Ukuphindaphinda kweqembu+isb A1, A2, A3; B1, B2, B3.
Ukuthunyelwa:
1. Iqhwa elomile: Amasampula adinga ukupakishwa ezikhwameni futhi agqitshwe eqhweni elomile.
2. Amashubhu e-RNAstable: Amasampula e-RNA angomiswa ngeshubhu lokuzinzisa le-RNA(isb. i-RNAstable®) futhi athunyelwe ekamelweni lokushisa.
Ukugeleza Komsebenzi Wesevisi
Idizayini yokuhlola
Ukulethwa kwesampula
Ukukhishwa kwe-RNA
Ukwakhiwa komtapo wolwazi
Ukulandelanisa
Ukuhlaziywa kwedatha
Amasevisi angemuva kokuthengisa
I-Bioinformatics
Ukuhlaziywa kwe-Differential Gene Expression (DEGs).
Ukulinganisa kwenkulumo ye-lncRNA - ukuhlanganisa
Ukunothiswa kwezakhi zofuzo ezihlosiwe ze-lncRNA
Ukuhlaziywa kwesikhundla se-mRNA kanye ne-lncRNA - Isakhiwo se-Circos (indingilizi ephakathi yi-mRNA kanti i-cicrlce yangaphakathi ithi lncRNA)
Hlola intuthuko egqugquzelwa izinsizakalo zokulinganisa ze-BMKGene's lncRNA ngeqoqo elikhethiwe lokushicilelwe.
Ji, H. et al. (2020) 'Ukuhlonza, ukubikezela okusebenzayo, nokuqinisekisa okubalulekile kwe-lncRNA kwama-lncRNA ahlobene nengcindezi ebandayo esibindini samagundane', Imibiko Yesayensi 2020 10:1, 10(1), pp. 1–14. doi: 10.1038/s41598-020-57451-7.
Jia, Z. et al. (2021) 'I-Integrative Transcriptomic Analysis Yembula Indlela Yokuzivikela Kumzimba Ye-CyHV-3-Resistant Common Carp Strain', Frontiers in Immunology, 12, p. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ et al. (2022) 'I-Multi-Omics-Integration-Based Priorization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Characteristics of Molecular and Drug Candidates', Frontiers in Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'Ukuhlukaniswa kofuzo kwenethiwekhi ye-gene coexpression network engaphansi kwe-photosynthesis ku-Populus', Ijenali ye-Plant Biotechnology, 18(4), pp. 1015–1026. doi: 10.1111/PBI.13270.
UZheng, H. et al. (2022) 'I-Global Regulatory Network for Dysregulated Gene Expression and Abnormal Metabolic Signaling in the Immune Cell in the Microenvironment of Graves' Disease kanye ne-Hashimoto's Thyroiditis', Frontiers in Immunology, 13, p. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
