Imikhiqizo

I-Genome-wide Association Analysis

Ucwaningo lwe-Genome-wide association (GWAS) luhlose ukuhlonza okuhlukile kofuzo (i-genotype) ehlotshaniswa nezici ezithile (i-phenotype).Ucwaningo lwe-GWAS luphenya izimpawu zofuzo eziwela lonke i-genome yenani elikhulu labantu futhi libikezela izinhlangano ze-genotype-phenotype ngokuhlaziywa kwezibalo ezingeni labantu.Isetshenziswe kabanzi ocwaningweni lwezifo zabantu kanye nokumbiwa kwezakhi zofuzo ezicini eziyinkimbinkimbi zezilwane noma izitshalo.

Xhumana nathi

Imininingwane Yesevisi

Umphumela wedemo

Ukushicilelwa Okufakiwe

1.Izinzuzo Zesevisi

● Inqwaba Yamacala Ephrojekthi: kusukela yasungulwa ngo-2009, i-BMKGENE isiqede amakhulukhulu ezinhlobo zezinhlobo ocwaningweni lwe-GWAS lwabantu, yasiza abacwaningi ukuthi bashicilele ama-athikili angaphezu kwe-100, futhi isici somthelela esikhulayo sifinyelele ku-500.
● Abahlaziyi abangochwepheshe.
● Umjikelezo wokuhlaziya omfushane.
● Ukumbiwa kwedatha okunembile.

2. Imininingwane Yesevisi

Uhlobo	Isikali sabantu	Isu lokulandelanisa nokujula
I-SLAF-GWAS	Inombolo yesampula ≥200	Usayizi weGenome <400M, one-ref-genome, i-WGS iyanconywa
		Usayizi we-genome ≤ 1G, 100K Tags kanye ne-10X
		1G ≤ Usayizi wegenome ≤ 2G, 200K Omaki no-10X
		Usayizi we-genome > 2G, 300K Tags kanye ne-10X
I-WGS-GWAS	Inombolo yesampula ≥200	10X ngesampula ngayinye

3. Ukukhetha Izinto

Izinhlobo ezehlukene, izinhlobonhlobo, ama-landraces/amabhange ofuzo/imindeni exubile/izinsiza zasendle

Izinhlobo ezahlukene, subspecies, landraces

Izinsiza zomndeni we-half-sib/full-sib/wild

4. Ukuhlaziywa kwe-Bioinformation

● Ukuhlaziywa kwenhlangano yofuzo olubanzi
● Ukuhlaziywa nokuhlolwa kwe-SNP ebalulekile
● Isichasiselo esisebenzayo sofuzo lwekhandidethi

5. Ukugeleza Komsebenzi Wenkonzo

Idizayini yokuhlola

Ukulethwa kwesampula

Ukukhishwa kwe-RNA

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukuhlaziywa kwedatha

Amasevisi angemuva kokuthengisa

Okwedlule: I-Single-nucleus RNA Sequencing

Olandelayo:

a.I-Phenotype QC

I-histogram yokusabalalisa imvamisa

Izibalo ze-Phenotype

b.Ukuhlaziywa kwenhlangano (Imodeli: GEMMA, FaST-LMM, EMMAX)

Idatha ye-QQ

Isakhiwo saseManhattan

Unyaka	Ijenali	IF	Isihloko
2022	NC	17.69	Isisekelo se-genomic se-giga-chromosomes kanye ne-giga-genome yesihlahla se-peony Paeonia ostii
2015	NP	7.43	Izinyathelo zasekhaya ziqinisa izifunda ze-genomic zokubaluleka kwe-agronomic kubhontshisi wesoya
2018	MP	9.32	Ukuphindaphinda kabusha kwegenome kweqoqo lomhlaba wonke le-rapeseed accessions kuveza isisekelo sofuzo sokuhlukana kwabo kwe-ecotype
2022	HR	7.29	Ukuhlaziywa kwe-genome-wide association kunikeza ukuqonda kwamangqamuzana ekuhlukeni kwemvelo kosayizi wembewu yekhabe