Ukuhlaziywa Kobudlelwano Bomhlaba Wonke be-Genome
Ukuhamba komsebenzi
Izinzuzo Zesevisi
●Ubuchwepheshe Obubanzi kanye namarekhodi okushicilela: ngolwazi oluqoqwe ku-GWAS, i-BMKGene isiqede amakhulu amaphrojekthi ezinhlobo ocwaningweni lwe-GWAS lwabantu, yasiza abacwaningi ukushicilela izihloko ezingaphezu kwe-100, kanti isici somthelela esihlanganisiwe safinyelela ku-500.
● Ukuhlaziywa okuphelele kwe-bioinformatics: ukuhamba komsebenzi kufaka phakathi ukuhlaziywa kokuhlobana kwezimpawu ze-SNP, okuletha isethi yezakhi zofuzo ezikhethiwe kanye nesichasiselo sazo sokusebenza esihambisanayo.
●Ithimba le-bioinformatics elinekhono eliphezulu kanye nomjikelezo omfushane wokuhlaziya: ngesipiliyoni esihle ekuhlaziyweni kwe-genomics okuthuthukisiwe, ithimba le-BMKGene lihlinzeka ngokuhlaziya okuphelele ngesikhathi sokushintsha okusheshayo.
●Usekelo Lwangemva Kokuthengisa:Ukuzibophezela kwethu kudlulela ngale kokuqedwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi zemibuzo nezimpendulo ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.
Imininingwane yesevisi kanye nezidingo
| Uhlobo lokulandelana | Isikali sabantu esinconywayo | Isu lokulandelana kwezigaba | Izidingo ze-Nucleotide |
| Ukulandelana Kwezakhi Zofuzo Okuphelele | Amasampula angu-200 | 10x | Ukugxila: ≥ 1 ng/ µL Inani eliphelele ≥ 30ng Ukuwohloka noma ukungcola okulinganiselwe noma okungenakho |
| I-Specific-Locus Amplified Fragment (SLAF) | Ukujula kwethegi: 10x Inani lamathegi: < 400 Mb: Kunconywa i-WGS < 1Gb: Amathegi angu-100K 1Gb > 2Gb: Amathegi angu-300K Amathegi aphezulu angu-500k | Ukugxila ≥ 5 ng/µL Inani eliphelele ≥ 80 ng Ijeli le-Agarose: akukho noma ukuwohloka okulinganiselwe noma ukungcola
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Ukukhethwa Kwezinto Ezibalulekile
Izinhlobo ezahlukene, izinhlobo ezincane, izindawo zokuhlala/ama-genebank/imindeni exubile/izinsiza zasendle
Izinhlobo ezahlukene, izinhlobo ezincane, izindawo zokuhlala
Umndeni womzali oyedwa/umndeni womzali oyedwa/izinsiza zasendle
Ukugeleza Komsebenzi Wesevisi
Umklamo wokuhlola
Ukulethwa kwesampula
Ukukhishwa kwe-RNA
Ukwakhiwa komtapo wolwazi
Ukulandelana
Ukuhlaziywa kwedatha
Izinsizakalo zangemuva kokuthengisa
Kufaka phakathi ukuhlaziywa okulandelayo:
- Ukuhlaziywa kokuhlanganiswa kwe-genome yonke: imodeli ye-LM, i-LMM, i-EMMAX, ne-FASTLMM
- Isichasiselo esisebenzayo sezakhi zofuzo ezingaba khona
Ukuhlaziywa kokuhlobana kwezimpawu ze-SNP - icebo laseManhattan
Ukuhlaziywa kokuhlobana kwezimpawu ze-SNP - icebo le-QQ
Hlola intuthuko eyenziwe yizinsizakalo ze-BMKGene's de GWAS ngokusebenzisa iqoqo lezincwadi ezikhethiwe:
Lv, L. et al. (2023) 'Ukuqonda ngesisekelo sezakhi zofuzo sokubekezelelana kwe-ammonia ku-razor clam Sinonovacula constricta ngocwaningo lokuhlobana kwezakhi zofuzo',Ukufuya izinhlanzi, 569, ikhasi 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
ULi, X. et al. (2022) 'Ukuhlaziywa kwe-Multi-omics kwe-398 foxtail millet accessions kwembula izifunda ze-genomic ezihlobene nokufakwa ekhaya, izici ze-metabolite, kanye nemiphumela yokulwa nokuvuvukala',Isitshalo Se-Molecular, 15(8), amakhasi 1367-1383. doi: 10.1016/j.molp.2022.07.003.
ULi, J. et al. (2022) 'Ukumapha Kokuhlobana Kofuzo Olubanzi Kwezinhlobo Ezingenazo Izinto Ezingavamile Emvelweni Yesomiso',Imingcele Yesayensi Yezitshalo, 13, ikhasi 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'I-GmST1, efaka ikhodi ye-sulfotransferase, inikeza ukumelana nezinhlobo zegciwane le-soya mosaic G2 kanye ne-G3',Isitshalo, Iseli kanye Nemvelo, 44(8), amakhasi 2777-2792. doi: 10.1111/PCE.14066.
