Ukulandelana kwe-Amplicon (16S/18S/ITS)

Ukulandelana kwe-Amplicon (16S/18S/ITS) ne-Illumina kuyindlela yokuhlaziya ukuhlukahluka kwamagciwane ngokuhlonza amaphrofayili amagciwane ngokwezinhlu zawo bese uzama ukuqonda ukuceba komphakathi kanye nokuhlukahluka ngaphakathi kwesampula ngayinye naphakathi kwamasampula. Ipayipi le-BMKCloud Amplicon (NGS) livumela ukuhlaziywa kwezakhi zofuzo ze-16S, 18S, ITS kanye nezinhlobo eziningi ezisebenzayo. Iqala ngokunciphisa ukufunda, ukuhlanganiswa kokufunda okuhlangene kanye nokuhlolwa kwekhwalithi, kulandelwe ukuhlanganiswa kokufundwa okufanayo ukuze kukhiqizwe ama-Operational Taxonomic Units (OTUs) asetshenziswa ezigabeni eziyisithupha ezahlukene zokuhlaziya. Isichasiselo se-Taxonomic sinikeza ulwazi ngobuningi obuhlobene kanye nokwakheka kwesampula ngayinye, kuyilapho ukuhlaziywa kokwehlukahluka kwe-alpha ne-beta kugxila ekuhlukeni kwamagciwane ngaphakathi naphakathi kwamasampula, ngokulandelana. Ukuhlaziywa kokwehluka phakathi kwamaqembu kuthola ama-OTU ahlukile kusetshenziswa kokubili ukuhlolwa kwe-parametric kanye nokungewona okwe-parametric, kuyilapho ukuhlaziywa kokuxhumana kuhlobanisa lokhu kwehluka nezici zemvelo. Okokugcina, ukugcwala kwezakhi zofuzo ezisebenzayo kubikezelwa ngokusekelwe ekugcwaleni kwezakhi zofuzo ezimaka, okunikeza ukuqonda ngomsebenzi kanye ne-ecology kusampula ngayinye.