Ubwoko bwa genotyping-cyane cyane kubantu benshi, nintambwe yingenzi mubushakashatsi bwamashyirahamwe, butanga ishingiro ryimiterere yo kuvumbura gene ikora, gusesengura ubwihindurize, nibindi. ) yatangijwe kugirango igabanye igiciro gikurikiranye kuri sample, mugihe gikomeza gukora neza kubuvumbuzi bwa geneti.Ibi mubisanzwe bigerwaho mugukuramo ibice byabuzanyijwe mubunini bwatanzwe, ibyo bikaba byaragabanijwe isomero ryerekana (RRL).Ibice byihariye-byongeweho ibice bikurikirana (SLAF-Seq) nuburyo bwateguwe bwo kuvumbura de novo SNP hamwe na genotyping ya SNP yabantu benshi.
Ibikorwa bya tekiniki
SLAF vs Uburyo bwa RRL buriho
Ibyiza bya SLAF
Ikirangantego cyo hejuru cyo kuvumbura neza- Hamwe na tekinoroji ikurikirana cyane, SLAF-Seq irashobora kugera ku bihumbi amagana bya tagi yavumbuwe muri genome yose kugirango isohoze icyifuzo cyimishinga itandukanye yubushakashatsi, haba hamwe na genome yerekanwe.
Igishushanyo mbonera cyoroshye- Kubwintego zitandukanye zubushakashatsi cyangwa ubwoko, ingamba zinyuranye zo gusya zirahari harimo-enzyme imwe, enzyme-ebyiri na enzyme nyinshi.Ingamba zo gusya zizasuzumwa mbere muri silico kugirango tumenye neza igishushanyo mbonera cya enzyme.
Gukora neza murwego rwo gusya- Igenamigambi ryateguwe neza ritanga SLAFs iringaniye kuri chromosome.Gukusanya ibice neza birashobora kugera kuri 95%.
Irinde gusubiramo- Ijanisha ryikurikiranya ryisubiramo mumibare ya SLAF-Seq ryaragabanutse kugera munsi ya 5%, cyane cyane mubinyabuzima bifite urwego rwo hejuru rwibintu bisubiramo, nk'ingano, ibigori, nibindi.
Kwikorera wenyine bioinformatic workflow- BMK yateje imbere ibikorwa bya bioinformatike ikoreshwa mubikorwa bya tekinoroji ya SLAF-Seq kugirango yizere neza kandi neza nibisohoka byanyuma.
Ikoreshwa rya SLAF
Ikarita ya genetike
Ikarita yerekana ubwinshi bwikarita yubaka no kumenyekanisha loci igenzura imiterere yindabyo muri Chrysanthemum (Chrysanthemum x morifolium Ramat.)
Ikinyamakuru: Ubushakashatsi bwimbuto bwimbuto bwatangajwe: 2020.7
GWAS
Kumenyekanisha umukandida gene ijyanye nibirimo isofavone mu mbuto za soya ukoresheje genome-rugari hamwe no guhuza amakarita
Ikinyamakuru: Ikinyamakuru cyibimera cyasohotse: 2020.08
Ubwihindurize
Isesengura rusange rya genomic hamwe na de novo inteko irerekana inkomoko yumuceri wibyatsi nkumukino wubwihindurize
Ikinyamakuru: Ibimera bya Molecular Byatangajwe: 2019.5
Isesengura ryinshi rya Segregant (BSA)
GmST1, ikubiyemo sulfotransferase, itanga imbaraga zo kurwanya virusi ya soya ya mosaic ya G2 na G3
Ikinyamakuru: Ibimera, Akagari & Ibidukikije Byatangajwe: 2021.04
Reba
Izuba X, Liu D, Zhang X, n'abandi.SLAF-Seq: uburyo bunoze bwo kuvumbura nini-nini ya SNP kuvumbura no gukoresha genotyping ukoresheje ibintu byinshi byinjira [J].Ikibanza kimwe, 2013, 8 (3): e58700
Indirimbo X, Xu Y, Gao K, n'abandi.Ikarita yerekana ubwinshi bwikarita yubaka no kumenyekanisha loci igenzura imiterere yindabyo muri Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020; 7: 108.
Wu D, Li D, Zhao X, n'abandi.Kumenyekanisha umukandida gene ifitanye isano nibirimo bya isoflavone mumbuto ya soya ukoresheje genome-rugari hamwe no guhuza amakarita.Igiterwa J. 2020;104 (4): 950-963.
Izuba J, Ma D, Tang L, n'abandi.Isesengura rusange ryabaturage hamwe na De Novo Inteko Yerekana Inkomoko yumuceri wibyatsi nkumukino wubwihindurize.Ibimera.2019; 12 (5): 632-647.Ibimera.2018;11 (11): 1360-1376.
Zhao X, Jing Y, Luo Z, n'abandi.GmST1, igizwe na sulfotransferase, itanga imbaraga zo kurwanya virusi ya soya ya G2 na G3.Gutera Ibidukikije.2021; 10.1111 / pce.14066
Igihe cyo kohereza: Mutarama-04-2022