I-genotyping ephezulu, ingakumbi kuluntu olukhulu, linyathelo elibalulekileyo kwizifundo zombutho wemfuza, obonelela ngesiseko sofuzo sofuzo olusebenzayo, uhlalutyo lwe-evolution, njl. Endaweni yolandelelwano olunzulu lwe-genome kwakhona, ukunciphisa ukumelwa kwe-genome sequencing (RRGS) ) yaziswa ukuze kuncitshiswe iindleko zolandelelwano ngesampulu nganye, ngeli lixa kugcinwe ukusebenza kakuhle ekufumaneni uphawu lwemfuzo.Oku kudla ngokuphunyezwa ngokutsala isiqwenga sothintelo ngaphakathi koluhlu lobungakanani obunikiweyo, esibizwa ngokuba yilayibrari yokubonisa encitshisiweyo (RRL).I-Specific-locus amplified fragment sequencing(SLAF-Seq) sisicwangciso esiziphuhlisileyo sokufunyanwa kwe-de novo SNP kunye ne-SNP genotyping yabemi abaninzi.
Ukuhamba kobugcisa
I-SLAF vs iindlela ezikhoyo ze-RRL
Izinto ezilungileyo zeSLAF
Ukufunyanwa kophawu oluphezulu lwemfuzo-Idityaniswe netekhnoloji yolandelelwano oluphezulu, i-SLAF-Seq inokufikelela kumakhulu amawaka eethegi ezifunyenwe kwi-genome epheleleyo ukuzalisekisa isicelo seeprojekthi zophando ezahlukeneyo, nokuba kunge-reference genome.
Uyilo lovavanyo olulungiselelweyo & olubhetyebhetye- Kwiinjongo ezahlukeneyo zophando okanye iintlobo, izicwangciso ezahlukeneyo zokugaya i-enzymatic ziyafumaneka kuquka i-enzyme enye, i-enzyme ephindwe kabini kunye ne-multi-enzyme digestion.Isicwangciso sokugaya siya kuvavanywa kwangaphambili kwi-silico ukuze kuqinisekiswe uyilo olufanelekileyo lwe-enzyme.
Ukusebenza okuphezulu kwi-enzymatic digestion– Ukwetyisa okulungiselelwe kwangaphambili kwe-enzymatic kubonelela ngokulinganayo ngakumbi ii-SLAFs kwichromosome.Ukuqokelelwa kwamaqhekeza ngokufanelekileyo kunokuzuza ngaphezulu kwe-95%.
Kuphephe ukuphindaphinda ulandelelwano- Ipesenti yokulandelelana ngokuphindaphindiweyo kwidatha ye-SLAF-Seq iyancipha ibe ngaphantsi kwe-5%, ngokukodwa kwiintlobo ezinezinga eliphezulu lezinto eziphindaphindiweyo, ezifana nengqolowa, umbona, njl.
Ukuziphuhlisa ngokwakho ukuhamba komsebenzi we-bioinformatiki-I-BMK iphuhlise ukuhamba komsebenzi okuhlanganisiweyo kwe-bioinformatic esebenzayo kwitekhnoloji ye-SLAF-Seq ukuqinisekisa ukuthembeka kunye nokuchaneka kwemveliso yokugqibela.
Ukusetyenziswa kweSLAF
Imephu yonxibelelwano lwemfuzo
Ulwakhiwo lwemephu yemfuza yoxinaniso oluphezulu kunye nokuchongwa kwe-loci elawula iimpawu zohlobo lweentyatyambo kwi-Chrysanthemum(Chrysanthemum x morifolium Ramat.)
Ijenali: UPhando lweHorticulture yapapashwa: 2020.7
I-GWAS
Ukuchongwa kofuzo lomgqatswa onxulunyaniswa nomxholo we-isofavone kwimbewu yeembotyi zesoya kusetyenziswa unxulumano olubanzi lwegenome kunye nemephu yonxibelelwano
Ijenali: Ijenali yeZityalo ipapashwe: 2020.08
I-Evolutionary Genetics
Uhlalutyo lwe-genomic yabemi kunye nendibano ye-de novo ityhila imvelaphi yerayisi enokhula njengomdlalo wokuziphendukela kwemvelo
Ijenali: Isityalo semolekyuli sipapashiwe: 2019.5
Uhlahlelo lwe-Bulked Segregant (BSA)
I-GmST1, efaka i-sulfotransferase, inika ukuxhathisa kwiintsholongwane ze-soybean mosaic virus i-G2 kunye ne-G3.
Ijenali: Isityalo, iSeli&Indalo eSingqongileyo ipapashiwe: 2021.04
Isalathiso
ILanga X, uLiu D, uZhang X, kunye nabanye.I-SLAF-Seq: indlela esebenzayo yokufunyanwa kwe-SNP enkulu ye-de novo kunye ne-genotyping usebenzisa ukulandelelana okuphezulu kwe-high-throughput [J].Plos one, 2013, 8(3):e58700
Ingoma X, Xu Y, Gao K, et al.Ukwakhiwa kwemephu yemfuzo exineneyo kakhulu kunye nokuchongwa kwe-loci elawula iimpawu zohlobo lweentyatyambo kwi-Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020;7:108.
Wu D, Li D, Zhao X, et al.Ukuchongwa kofuzo lomgqatswa onxulunyaniswa nomxholo we-isoflavone kwimbewu yeembotyi zesoya kusetyenziswa unxulumano olubanzi lwegenome kunye nemephu yonxibelelwano.Isityalo J. 2020;104(4): 950-963.
Sun J, Ma D, Tang L, et al.Uhlalutyo lweGenomic yabemi kunye neNdibano yaseDe Novo ityhila iMvelaphi ye-Weedy Rice njengoMdlalo wokuZiphendukela kwemvelo.Isityalo saseMol.2019;12(5):632-647.Isityalo saseMol.2018;11(11): 1360-1376.
Zhao X, Jing Y, Luo Z, et al.I-GmST1, efaka i-sulfotransferase, inika ukuxhathisa kwiintsholongwane ze-soybean mosaic virus i-G2 kunye ne-G3.Plant Cell Environ.2021;10.1111/pce.14066
Ixesha lokuposa: Jan-04-2022