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Kusangana kweChromosome-scale uye kuongororwa kwechirimwa chebiomass Miscanthus lutarioriparius genome

Nanopore sequencing |Ilumina |Hi-C |RNA-sequencing |Phylogeny

Muchidzidzo ichi, Biomarker Technologies yakapa rutsigiro rwehunyanzvi paNanopore sequencing, de novo genome assembly, Hi-C yakabatsirwa gungano, nezvimwe.

Abstract

Miscanthus, a rhizomatous perennial chirimwa, ine mukana wakakura wekugadzira bioenergy kune yakakwira biomass uye kusagadzikana kushivirira.Isu tinoshuma kusangana kwechromosome-scale yeMiscanthus lutarioripariusgenome nekubatanidza Oxford Nanopore kutevedzana uye Hi-C matekinoroji.Musangano we2.07-Gb unovhara 96.64% yegenome, ine contig N50 ye1.71 Mb.Iyo centromere uye telomere sequences dzakaunganidzwa kune ese gumi nemapfumbamwe machromosomes uye chromosome gumi, zvichiteerana.Mabviro eAllotetraploid yeM. lutarioriparius inosimbiswa pachishandiswa centromeric satellite repeats.Iyo tetraploid genome chimiro uye akati wandei chromosomal rearrangements zvine chekuita nemhunga zvinoratidzwa zvakajeka.Tandem duplicate majini eM. lutarioripariusanoshanda akafumiswa kwete chete mumashoko ane chekuita nekunetsekana kupindura, asi cell wall biosynthesis.Mhuri dzeGene dzine hukama nekurwisa chirwere, sero madziro biosynthesis uye simbi ion yekutakura inowedzerwa zvakanyanya uye yakashanduka.Kuwedzera kwemhuri idzi kunogona kuve kwakakosha genomic hwaro hwekusimudzira hunhu hunoshamisa hweM. lutarioriparius.

Nhamba dzakakosha dzegungano rejenome

nhau-1
nhau-2

Mufananidzo.Mhedziso yeM. lutarioriparius genome assembly

Nhau Nezvakakosha inovavarira kugovera nyaya dzichangoburwa dzakabudirira neBiomarker Technologies, kutora budiriro yesainzi pamwe nemaitiro ane mukurumbira akashandiswa panguva yekudzidza.


Nguva yekutumira: Jan-05-2022

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