One important horticultural characteristic that distinguishes navel oranges from other common sweet orange varieties is the presence of a navel on the fruit. This feature is also an important criterion for the classification of sweet orange horticultural varieties.
BMKGENE’s clients utilized BMKGENE’s self-developed SLAF sequencing technology to conduct a study titled “Mining Genes Related to Fruit Quality in Sweet Oranges Based on Specific Locus Amplified Fragment Sequencing.“
The study involved sequencing 240 sweet orange germplasm resources with wide genetic diversity and different geographic origins, resulting in 497.82 Mb of short-read data. Analysis of the data yielded 1,467,968 SNP genotypes across the entire genome. By employing Fst analysis, the study identified 6 candidate genes related to the presence of a navel, fruit weight, and titratable acidity, providing a basis for targeted improvement of sweet orange varieties.
For large-scale population sequencing, specific locus amplified fragment sequencing (SLAF) is more cost-effective and high-effective compared to whole-genome sequencing (WGS).
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Post time: Dec-05-2023